Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells
This data article describes cellular dynamics, such as migration speed and mobility of the cytoskeletal protein, of wild-type human fibroblast cells and cells with a modified adeno-associated virus integration site 1 (AAVS1) locus on human chromosome 19. Insertion of exogenous gene into the AAVS1 lo...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340916000044 |
| _version_ | 1818477029738151936 |
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| author | Takeomi Mizutani Hisashi Haga Kazushige Kawabata |
| author_facet | Takeomi Mizutani Hisashi Haga Kazushige Kawabata |
| author_sort | Takeomi Mizutani |
| collection | DOAJ |
| description | This data article describes cellular dynamics, such as migration speed and mobility of the cytoskeletal protein, of wild-type human fibroblast cells and cells with a modified adeno-associated virus integration site 1 (AAVS1) locus on human chromosome 19. Insertion of exogenous gene into the AAVS1 locus has been conducted in recent biological researches. Previously, our data showed that the AAVS1-modification changes cellular contractile force (Mizutani et al., 2015 [1]). To assess if this AAVS1-modification affects cell migration, we compared cellular migration speed and turnover of cytoskeletal protein in human fibroblasts and fibroblasts with a green fluorescent protein gene knocked-in at the AAVS1 locus in this data article. Cell nuclei were stained and changes in their position attributable to cell migration were analyzed. Fluorescence recovery was observed after photobleaching for the fluorescent protein-tagged myosin regulatory light chain. Data here are related to the research article “Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85” [1]. Keywords: Genome editing, Myosin regulatory light chain, Cell migration, Mean square displacement |
| first_indexed | 2024-12-10T09:32:49Z |
| format | Article |
| id | doaj.art-71ad5426b5b3494caf62d6d257a8b430 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-10T09:32:49Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-71ad5426b5b3494caf62d6d257a8b4302022-12-22T01:54:17ZengElsevierData in Brief2352-34092016-03-016793798Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cellsTakeomi Mizutani0Hisashi Haga1Kazushige Kawabata2Corresponding author.; Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, JapanDepartment of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, JapanDepartment of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, JapanThis data article describes cellular dynamics, such as migration speed and mobility of the cytoskeletal protein, of wild-type human fibroblast cells and cells with a modified adeno-associated virus integration site 1 (AAVS1) locus on human chromosome 19. Insertion of exogenous gene into the AAVS1 locus has been conducted in recent biological researches. Previously, our data showed that the AAVS1-modification changes cellular contractile force (Mizutani et al., 2015 [1]). To assess if this AAVS1-modification affects cell migration, we compared cellular migration speed and turnover of cytoskeletal protein in human fibroblasts and fibroblasts with a green fluorescent protein gene knocked-in at the AAVS1 locus in this data article. Cell nuclei were stained and changes in their position attributable to cell migration were analyzed. Fluorescence recovery was observed after photobleaching for the fluorescent protein-tagged myosin regulatory light chain. Data here are related to the research article “Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85” [1]. Keywords: Genome editing, Myosin regulatory light chain, Cell migration, Mean square displacementhttp://www.sciencedirect.com/science/article/pii/S2352340916000044 |
| spellingShingle | Takeomi Mizutani Hisashi Haga Kazushige Kawabata Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells Data in Brief |
| title | Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells |
| title_full | Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells |
| title_fullStr | Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells |
| title_full_unstemmed | Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells |
| title_short | Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells |
| title_sort | data set for comparison of cellular dynamics between human aavs1 locus modified and wild type cells |
| url | http://www.sciencedirect.com/science/article/pii/S2352340916000044 |
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