Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae)
Abstract Background Topical application of the entomopathogenic fungi (EPFs) against Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae is less effective due to larvae hiding in the corn midribs in the field. To control the larvae, the fungi colonize in plant tissues or endophytic fung...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-08-01
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| Series: | Egyptian Journal of Biological Pest Control |
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| Online Access: | https://doi.org/10.1186/s41938-022-00605-8 |
| _version_ | 1818082579736166400 |
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| author | Jelly Milinia Puspita Sari Siti Herlinda Suwandi Suwandi |
| author_facet | Jelly Milinia Puspita Sari Siti Herlinda Suwandi Suwandi |
| author_sort | Jelly Milinia Puspita Sari |
| collection | DOAJ |
| description | Abstract Background Topical application of the entomopathogenic fungi (EPFs) against Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae is less effective due to larvae hiding in the corn midribs in the field. To control the larvae, the fungi colonize in plant tissues or endophytic fungi are needed. There is no information on the pathogenicity of the endophytic fungi from Indonesia on the development of S. frugiperda. The endophytic fungi isolated from infected-host cadavers from South Sumatra (Indonesia) were identified morphologically and molecularly, and the effect of seed-treated corn seedlings with the fungi on S. frugiperda development was evaluated. The fungal identification was based on morphological and molecular characteristics. Bioassay of the endophytic fungal species in seed-treated young maize was performed against the neonate larvae (hatching within 24 h.) of first instar, and their development was observed. Results The results of molecular identification showed that the fungal species were Beauveria bassiana of five fungal isolates (WTTJC290521B, WTTJC290521A, JGTP240521A, JGNT300521, and WTTJC260521A) and Metarhizium anisopliae of an isolate (WTTJC260521B). The life span of S. frugiperda fed on leaves of fungal-colonized maize was significantly longer than those fed on leaves of non-colonized maize. The fungal-colonized young maize significantly increased mortality rate of all larval instars than non-colonized one. The last instar larvae mortality treated with B. bassiana (JGTP240521A isolates) (51.33%) was the highest among other treatments and did not significantly differ from each of B. bassiana of WTTJC260521A and WTTJC290521A isolates 45.33% and 44.67%, respectively. Feeding on leaves of fungal-colonized maize significantly decreased the percentage of the last instar larvae development to the pupal stage, the adult emergence, the eggs laid, and the percentage of hatched eggs. This is the first report that B. bassiana and M. anisopliae from South Sumatra (Indonesia) in seed-treated corn seedlings had negative effects on development of S. frugiperda. Conclusions Finally, these results highlight the potential of endophytic EPFs to protect corn plants against S. frugiperda. |
| first_indexed | 2024-12-10T19:24:21Z |
| format | Article |
| id | doaj.art-71adb1e0594946de8ea544e6760eb4f6 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-10T19:24:21Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-71adb1e0594946de8ea544e6760eb4f62022-12-22T01:36:24ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-08-0132111110.1186/s41938-022-00605-8Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae)Jelly Milinia Puspita Sari0Siti Herlinda1Suwandi Suwandi2Graduate Program of Crop Sciences, Faculty of Agriculture, Universitas SriwijayaGraduate Program of Crop Sciences, Faculty of Agriculture, Universitas SriwijayaGraduate Program of Crop Sciences, Faculty of Agriculture, Universitas SriwijayaAbstract Background Topical application of the entomopathogenic fungi (EPFs) against Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae is less effective due to larvae hiding in the corn midribs in the field. To control the larvae, the fungi colonize in plant tissues or endophytic fungi are needed. There is no information on the pathogenicity of the endophytic fungi from Indonesia on the development of S. frugiperda. The endophytic fungi isolated from infected-host cadavers from South Sumatra (Indonesia) were identified morphologically and molecularly, and the effect of seed-treated corn seedlings with the fungi on S. frugiperda development was evaluated. The fungal identification was based on morphological and molecular characteristics. Bioassay of the endophytic fungal species in seed-treated young maize was performed against the neonate larvae (hatching within 24 h.) of first instar, and their development was observed. Results The results of molecular identification showed that the fungal species were Beauveria bassiana of five fungal isolates (WTTJC290521B, WTTJC290521A, JGTP240521A, JGNT300521, and WTTJC260521A) and Metarhizium anisopliae of an isolate (WTTJC260521B). The life span of S. frugiperda fed on leaves of fungal-colonized maize was significantly longer than those fed on leaves of non-colonized maize. The fungal-colonized young maize significantly increased mortality rate of all larval instars than non-colonized one. The last instar larvae mortality treated with B. bassiana (JGTP240521A isolates) (51.33%) was the highest among other treatments and did not significantly differ from each of B. bassiana of WTTJC260521A and WTTJC290521A isolates 45.33% and 44.67%, respectively. Feeding on leaves of fungal-colonized maize significantly decreased the percentage of the last instar larvae development to the pupal stage, the adult emergence, the eggs laid, and the percentage of hatched eggs. This is the first report that B. bassiana and M. anisopliae from South Sumatra (Indonesia) in seed-treated corn seedlings had negative effects on development of S. frugiperda. Conclusions Finally, these results highlight the potential of endophytic EPFs to protect corn plants against S. frugiperda.https://doi.org/10.1186/s41938-022-00605-8Spodoptera frugiperdaBeauveria bassianaMetarhizium anisopliaeEndophyteEntomopathogens |
| spellingShingle | Jelly Milinia Puspita Sari Siti Herlinda Suwandi Suwandi Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) Egyptian Journal of Biological Pest Control Spodoptera frugiperda Beauveria bassiana Metarhizium anisopliae Endophyte Entomopathogens |
| title | Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) |
| title_full | Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) |
| title_fullStr | Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) |
| title_full_unstemmed | Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) |
| title_short | Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn seedlings Affecting development of the fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) |
| title_sort | endophytic fungi from south sumatra indonesia in seed treated corn seedlings affecting development of the fall armyworm spodoptera frugiperda j e smith lepidoptera noctuidae |
| topic | Spodoptera frugiperda Beauveria bassiana Metarhizium anisopliae Endophyte Entomopathogens |
| url | https://doi.org/10.1186/s41938-022-00605-8 |
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