Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays
We conducted a rapid bioassay method to assess insecticide efficacy for controlling adult sweetpotato whitefly <i>Bemisia tabaci</i> in squash and cucumber crops before insecticide applications. The study aimed to evaluate the accuracy of a 24-hour laboratory bioassay in determining maxi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/6/510 |
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| author | Paulo S. G. Cremonez Jermaine D. Perier Alvin M. Simmons David G. Riley |
| author_facet | Paulo S. G. Cremonez Jermaine D. Perier Alvin M. Simmons David G. Riley |
| author_sort | Paulo S. G. Cremonez |
| collection | DOAJ |
| description | We conducted a rapid bioassay method to assess insecticide efficacy for controlling adult sweetpotato whitefly <i>Bemisia tabaci</i> in squash and cucumber crops before insecticide applications. The study aimed to evaluate the accuracy of a 24-hour laboratory bioassay in determining maximum dose insecticide efficacy in the field. Ten insecticides were evaluated using leaf-dip bioassays, and their effectiveness was tested across eight cucurbit field experiments in Georgia, USA, during the 2021 and 2022 field seasons. The maximum dose, defined as the highest labeled rate of an insecticide diluted in the equivalent of 935 L ha<sup>−1</sup> of water, was used for all bioassays. Adult survival observed in the bioassay was compared to adult field count-based survival 24 h after treatment. A low concentration (1/10 rate) was used for imidacloprid, flupyradifurone, pyriproxyfen, and cyantraniliprole to assess insecticide tolerance in the whitefly population. Overall, significant positive correlation between laboratory bioassay and field efficacy was reported, explaining 50–91% of the observed variation. The addition of the low dosage was helpful, indicating that no rate response was consistent with susceptibility to the tested insecticide, while a rate response was associated with a loss of susceptibility between 2021 and 2022. |
| first_indexed | 2024-03-11T02:19:25Z |
| format | Article |
| id | doaj.art-71ae7b52be07482684f6db50b47e53f2 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T02:19:25Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-71ae7b52be07482684f6db50b47e53f22023-11-18T10:55:49ZengMDPI AGInsects2075-44502023-06-0114651010.3390/insects14060510Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose BioassaysPaulo S. G. Cremonez0Jermaine D. Perier1Alvin M. Simmons2David G. Riley3Department of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USADepartment of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USAU.S. Vegetable Laboratory, Agricultural Research Service, United States Department of Agriculture, 2700 Savannah Highway, Charleston, SC 29414, USADepartment of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USAWe conducted a rapid bioassay method to assess insecticide efficacy for controlling adult sweetpotato whitefly <i>Bemisia tabaci</i> in squash and cucumber crops before insecticide applications. The study aimed to evaluate the accuracy of a 24-hour laboratory bioassay in determining maximum dose insecticide efficacy in the field. Ten insecticides were evaluated using leaf-dip bioassays, and their effectiveness was tested across eight cucurbit field experiments in Georgia, USA, during the 2021 and 2022 field seasons. The maximum dose, defined as the highest labeled rate of an insecticide diluted in the equivalent of 935 L ha<sup>−1</sup> of water, was used for all bioassays. Adult survival observed in the bioassay was compared to adult field count-based survival 24 h after treatment. A low concentration (1/10 rate) was used for imidacloprid, flupyradifurone, pyriproxyfen, and cyantraniliprole to assess insecticide tolerance in the whitefly population. Overall, significant positive correlation between laboratory bioassay and field efficacy was reported, explaining 50–91% of the observed variation. The addition of the low dosage was helpful, indicating that no rate response was consistent with susceptibility to the tested insecticide, while a rate response was associated with a loss of susceptibility between 2021 and 2022.https://www.mdpi.com/2075-4450/14/6/510insecticide resistance management (IRM)maximum-dose bioassaysweetpotato whitefly<i>Bemisia tabaci</i> |
| spellingShingle | Paulo S. G. Cremonez Jermaine D. Perier Alvin M. Simmons David G. Riley Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays Insects insecticide resistance management (IRM) maximum-dose bioassay sweetpotato whitefly <i>Bemisia tabaci</i> |
| title | Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays |
| title_full | Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays |
| title_fullStr | Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays |
| title_full_unstemmed | Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays |
| title_short | Determining Field Insecticide Efficacy on Whiteflies with Maximum Dose Bioassays |
| title_sort | determining field insecticide efficacy on whiteflies with maximum dose bioassays |
| topic | insecticide resistance management (IRM) maximum-dose bioassay sweetpotato whitefly <i>Bemisia tabaci</i> |
| url | https://www.mdpi.com/2075-4450/14/6/510 |
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