Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase
The data shown in this article are related to the published paper entitled “A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate” in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purificatio...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923002585 |
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| author | Lin Wei Yingying Xu Min Du Ying Fan Ruyi Zou Xiangyu Xu Qingdong Zhang Yu-Zhong Zhang Wenshuang Wang Fuchuan Li |
| author_facet | Lin Wei Yingying Xu Min Du Ying Fan Ruyi Zou Xiangyu Xu Qingdong Zhang Yu-Zhong Zhang Wenshuang Wang Fuchuan Li |
| author_sort | Lin Wei |
| collection | DOAJ |
| description | The data shown in this article are related to the published paper entitled “A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate” in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI4SF with a molecular mass of 59.13 kDa can can specifically hydrolyze the 4-O- but not 2-O- and 6-O-sulfate groups in the oligo-/polysaccharides of chondroitin sulfate/dermatan sulfate and show the maximum reaction rate in 50 mM Tris-HCl buffer (pH 7.0) at 50°C, which can be a very useful tool for the structural and functional studies of chondroitin sulfate/dermatan sulfate. |
| first_indexed | 2024-03-13T03:58:02Z |
| format | Article |
| id | doaj.art-71aebe7b34eb432db872ab08d71b2852 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-03-13T03:58:02Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-71aebe7b34eb432db872ab08d71b28522023-06-22T05:03:43ZengElsevierData in Brief2352-34092023-06-0148109139Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfataseLin Wei0Yingying Xu1Min Du2Ying Fan3Ruyi Zou4Xiangyu Xu5Qingdong Zhang6Yu-Zhong Zhang7Wenshuang Wang8Fuchuan Li9National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, ChinaNational Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, ChinaNational Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, ChinaQingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao 266071, ChinaNational Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, ChinaNational Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, ChinaSchool of Life Science and Technology, Weifang Medical University, 7166 Baotong West Street, Weifang 261053, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, ChinaNational Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, China; Corresponding authors.National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, China; College of Marine Life Sciences, Ocean University of China, Qingdao, China; Corresponding authors.The data shown in this article are related to the published paper entitled “A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate” in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI4SF with a molecular mass of 59.13 kDa can can specifically hydrolyze the 4-O- but not 2-O- and 6-O-sulfate groups in the oligo-/polysaccharides of chondroitin sulfate/dermatan sulfate and show the maximum reaction rate in 50 mM Tris-HCl buffer (pH 7.0) at 50°C, which can be a very useful tool for the structural and functional studies of chondroitin sulfate/dermatan sulfate.http://www.sciencedirect.com/science/article/pii/S2352340923002585SulfataseCloning and expressionBiochemical characteristicsSubstrate specificity |
| spellingShingle | Lin Wei Yingying Xu Min Du Ying Fan Ruyi Zou Xiangyu Xu Qingdong Zhang Yu-Zhong Zhang Wenshuang Wang Fuchuan Li Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase Data in Brief Sulfatase Cloning and expression Biochemical characteristics Substrate specificity |
| title | Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase |
| title_full | Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase |
| title_fullStr | Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase |
| title_full_unstemmed | Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase |
| title_short | Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase |
| title_sort | data on cloning expression and biochemical characteristics of a chondroitin sulfate dermatan sulfate 4 o endosulfatase |
| topic | Sulfatase Cloning and expression Biochemical characteristics Substrate specificity |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923002585 |
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