Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study
Background: Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-α activity and polymorphism in TNFR1 could lead to gene dysfunction a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2017-09-01
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| Series: | International Journal of Reproductive BioMedicine |
| Online Access: | http://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-56&slc_lang=en&sid=1 |
| _version_ | 1829478930415878144 |
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| author | Hamid Reza Ashrafzadeh Tahere Nazari Masoud Dehghan Tezerjani Maryam Khademi Bami Saeed Ghasemi-Esmailabad Nasrin Ghasemi |
| author_facet | Hamid Reza Ashrafzadeh Tahere Nazari Masoud Dehghan Tezerjani Maryam Khademi Bami Saeed Ghasemi-Esmailabad Nasrin Ghasemi |
| author_sort | Hamid Reza Ashrafzadeh |
| collection | DOAJ |
| description | Background: Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-α activity and polymorphism in TNFR1 could lead to gene dysfunction and male infertility. Objective: The aim of this study is to determine the association of TNFR1 36 A/G polymorphism with the idiopathic azoospermia in Iranian population. Materials and Methods: This case-control study included 108 azoospermic and 119 fertile men. This research investigated the frequency of TNFR1 36 A/G polymorphism in cases who were idiopathic azoospermic men referred to Yazd Research and Clinical Center for Infertility, Iran in comparison with controls. polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the polymorphism in both case and control groups. PCR fragments were digested by Mspa1I enzyme and products were appeared by gel electrophoresis. The abundance of A→G was calculated in the azoospermic and healthy men. Results: According to the present study, GG and AG genotypes frequency in the azoospermic men group were higher than the control group (OR= 2.298 (1.248-4.229), p=0.007), (OR=1.47 (0.869-2.498, p=0.149). Our findings also showed that G allele frequency in azoospermic men had significant difference compared to the control group (OR=2.302 (1.580-3.355), p<0.001). Conclusion: It seems that the GG genotype and G allele have an association with increased risk of non-obstructive azoospermia |
| first_indexed | 2024-12-14T17:26:01Z |
| format | Article |
| id | doaj.art-71b0bc20295b41e6bdedc93752c521c0 |
| institution | Directory Open Access Journal |
| issn | 2476-3772 2476-3772 |
| language | English |
| last_indexed | 2024-12-14T17:26:01Z |
| publishDate | 2017-09-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-71b0bc20295b41e6bdedc93752c521c02022-12-21T22:53:13ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-37722476-37722017-09-01158521526Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control studyHamid Reza Ashrafzadeh0Tahere Nazari1Masoud Dehghan Tezerjani2Maryam Khademi Bami3Saeed Ghasemi-Esmailabad4Nasrin Ghasemi5 Background: Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-α activity and polymorphism in TNFR1 could lead to gene dysfunction and male infertility. Objective: The aim of this study is to determine the association of TNFR1 36 A/G polymorphism with the idiopathic azoospermia in Iranian population. Materials and Methods: This case-control study included 108 azoospermic and 119 fertile men. This research investigated the frequency of TNFR1 36 A/G polymorphism in cases who were idiopathic azoospermic men referred to Yazd Research and Clinical Center for Infertility, Iran in comparison with controls. polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the polymorphism in both case and control groups. PCR fragments were digested by Mspa1I enzyme and products were appeared by gel electrophoresis. The abundance of A→G was calculated in the azoospermic and healthy men. Results: According to the present study, GG and AG genotypes frequency in the azoospermic men group were higher than the control group (OR= 2.298 (1.248-4.229), p=0.007), (OR=1.47 (0.869-2.498, p=0.149). Our findings also showed that G allele frequency in azoospermic men had significant difference compared to the control group (OR=2.302 (1.580-3.355), p<0.001). Conclusion: It seems that the GG genotype and G allele have an association with increased risk of non-obstructive azoospermiahttp://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-56&slc_lang=en&sid=1 |
| spellingShingle | Hamid Reza Ashrafzadeh Tahere Nazari Masoud Dehghan Tezerjani Maryam Khademi Bami Saeed Ghasemi-Esmailabad Nasrin Ghasemi Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study International Journal of Reproductive BioMedicine |
| title | Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study |
| title_full | Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study |
| title_fullStr | Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study |
| title_full_unstemmed | Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study |
| title_short | Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study |
| title_sort | frequency of tnfr1 36 a g gene polymorphism in azoospermic infertile men a case control study |
| url | http://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-56&slc_lang=en&sid=1 |
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