Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies
To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse tr...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/1/80 |
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| author | Xin Li Chenying Duan Ruyi Li Dong Wang |
| author_facet | Xin Li Chenying Duan Ruyi Li Dong Wang |
| author_sort | Xin Li |
| collection | DOAJ |
| description | To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional <i>ART3</i> gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression. |
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| id | doaj.art-71b3db19de9645c9aa9147d999751c41 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:29:04Z |
| publishDate | 2021-01-01 |
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| series | Animals |
| spelling | doaj.art-71b3db19de9645c9aa9147d999751c412023-11-21T08:18:54ZengMDPI AGAnimals2076-26152021-01-011118010.3390/ani11010080Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic StudiesXin Li0Chenying Duan1Ruyi Li2Dong Wang3Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaTo reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional <i>ART3</i> gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression.https://www.mdpi.com/2076-2615/11/1/80spermiogenesisdifferentially expressed geneshomology trends analysis |
| spellingShingle | Xin Li Chenying Duan Ruyi Li Dong Wang Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies Animals spermiogenesis differentially expressed genes homology trends analysis |
| title | Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies |
| title_full | Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies |
| title_fullStr | Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies |
| title_full_unstemmed | Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies |
| title_short | Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies |
| title_sort | insights into the mechanism of bovine spermiogenesis based on comparative transcriptomic studies |
| topic | spermiogenesis differentially expressed genes homology trends analysis |
| url | https://www.mdpi.com/2076-2615/11/1/80 |
| work_keys_str_mv | AT xinli insightsintothemechanismofbovinespermiogenesisbasedoncomparativetranscriptomicstudies AT chenyingduan insightsintothemechanismofbovinespermiogenesisbasedoncomparativetranscriptomicstudies AT ruyili insightsintothemechanismofbovinespermiogenesisbasedoncomparativetranscriptomicstudies AT dongwang insightsintothemechanismofbovinespermiogenesisbasedoncomparativetranscriptomicstudies |