Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags
The diversity and load of heterotrophic bacteria and fungi associated with the mangrove soil from Suva, Fiji Islands, was determined by using the plate count method. The ability of the bacterial isolates to produce various hydrolytic enzymes such as amylase, gelatinase and lipase were determined usi...
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| Format: | Article |
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Vicerractoría Investigación
2007-12-01
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| Series: | Revista de Biología Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442007000300004 |
| _version_ | 1797762657748516864 |
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| author | Shristi Kumar A.A.M Hatha K.S Christi |
| author_facet | Shristi Kumar A.A.M Hatha K.S Christi |
| author_sort | Shristi Kumar |
| collection | DOAJ |
| description | The diversity and load of heterotrophic bacteria and fungi associated with the mangrove soil from Suva, Fiji Islands, was determined by using the plate count method. The ability of the bacterial isolates to produce various hydrolytic enzymes such as amylase, gelatinase and lipase were determined using the plate assay. The heterotrophic bacterial load was considerably higher than the fungal load. There was a predominance of the gram positive genus, Bacillus. Other genera encountered included Staphylococcus, Micrococcus, Listeria and Vibrio. Their effectiveness on the degradation of commercial polythene carry bags made of high density polyethylene (HDPE) and low density polyethylene (LDPE) was studied over a period of eight weeks in the laboratory. Biodegradation was measured in terms of mean weight loss, which was nearly 5 % after a period of eight weeks. There was a significant increase in the bacterial load of the soil attached to class 2 (HDPE) polythene. After eight weeks of submergence in mangrove soil, soil attached to class 1 and class 3 polythene mostly had Bacillus (Staphylococcus predominated in class 2 polythene). While most of the isolates were capable of producing hydrolytic enzymes such as amylase and gelatinase, lipolytic activity was low. Class 2 HDPE suffered the greatest biodegradation. Rev. Biol. Trop. 55 (3-4): 777-786. Epub 2007 December, 28.<br>Se determinó la diversidad y la carga de bacterias heterotróficas, así como los hongos asociados al suelo del manglar de Suva, Islas Fiji, utilizando el método de conteo de placas, usado también para medir la capacidad de bacterias aisladas para producir enzimas hidrolíticas como amilasa, gelatinasa y lipasa. La carga bacteriana heterotrófica resultó ser considerablemente más alta que la carga funguicida. Hubo predominancia de bacterias "Gram-positivas" del género de Bacillus. Otros géneros encontrados fueron Staphylococcus, Micrococcus, Listeria y Vibrio. La eficacia de esta microflora en la degradación del polietileno comercial de bolsas hechas de polietileno de alta densidad (HDPE) y de baja densidad (LDPE) fue estudiada en el laboratorio por un periodo de ocho semanas. La biodegradación fue medida en términos de pérdida de peso, la cual indicó una disminución del 5 %. Después de ocho semanas en el suelo de un manglar, el polietileno clase 1 y clase 3 contenía fundamentalmente Bacillus, pero en el polietileno clase 2 predominó el género Staphylococcus. Mientras que la mayoría de bacterias aisladas fueron capaces de producir enzimas hidrolíticas como la amilasa y la gelatinasa, la actividad lipolítica fue muy baja. La clase 2 (HDPE) experimentó la mayor biodegradación. |
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| institution | Directory Open Access Journal |
| issn | 0034-7744 2215-2075 |
| language | English |
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| publishDate | 2007-12-01 |
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| series | Revista de Biología Tropical |
| spelling | doaj.art-71b48af31cd6446b9a9f1e1430970f3d2023-08-02T04:33:16ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752007-12-01553-4777786Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bagsShristi KumarA.A.M HathaK.S ChristiThe diversity and load of heterotrophic bacteria and fungi associated with the mangrove soil from Suva, Fiji Islands, was determined by using the plate count method. The ability of the bacterial isolates to produce various hydrolytic enzymes such as amylase, gelatinase and lipase were determined using the plate assay. The heterotrophic bacterial load was considerably higher than the fungal load. There was a predominance of the gram positive genus, Bacillus. Other genera encountered included Staphylococcus, Micrococcus, Listeria and Vibrio. Their effectiveness on the degradation of commercial polythene carry bags made of high density polyethylene (HDPE) and low density polyethylene (LDPE) was studied over a period of eight weeks in the laboratory. Biodegradation was measured in terms of mean weight loss, which was nearly 5 % after a period of eight weeks. There was a significant increase in the bacterial load of the soil attached to class 2 (HDPE) polythene. After eight weeks of submergence in mangrove soil, soil attached to class 1 and class 3 polythene mostly had Bacillus (Staphylococcus predominated in class 2 polythene). While most of the isolates were capable of producing hydrolytic enzymes such as amylase and gelatinase, lipolytic activity was low. Class 2 HDPE suffered the greatest biodegradation. Rev. Biol. Trop. 55 (3-4): 777-786. Epub 2007 December, 28.<br>Se determinó la diversidad y la carga de bacterias heterotróficas, así como los hongos asociados al suelo del manglar de Suva, Islas Fiji, utilizando el método de conteo de placas, usado también para medir la capacidad de bacterias aisladas para producir enzimas hidrolíticas como amilasa, gelatinasa y lipasa. La carga bacteriana heterotrófica resultó ser considerablemente más alta que la carga funguicida. Hubo predominancia de bacterias "Gram-positivas" del género de Bacillus. Otros géneros encontrados fueron Staphylococcus, Micrococcus, Listeria y Vibrio. La eficacia de esta microflora en la degradación del polietileno comercial de bolsas hechas de polietileno de alta densidad (HDPE) y de baja densidad (LDPE) fue estudiada en el laboratorio por un periodo de ocho semanas. La biodegradación fue medida en términos de pérdida de peso, la cual indicó una disminución del 5 %. Después de ocho semanas en el suelo de un manglar, el polietileno clase 1 y clase 3 contenía fundamentalmente Bacillus, pero en el polietileno clase 2 predominó el género Staphylococcus. Mientras que la mayoría de bacterias aisladas fueron capaces de producir enzimas hidrolíticas como la amilasa y la gelatinasa, la actividad lipolítica fue muy baja. La clase 2 (HDPE) experimentó la mayor biodegradación.http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442007000300004Suelo de manglarbacteria heterotróficacontaminación plásticadegradación microbianabiodegradaciónenzimas hidrolíticasMangrove soilheterotrophic bacteriaplastic pollutionmicrobial degradationbiodegradationhydrolytic enzymes |
| spellingShingle | Shristi Kumar A.A.M Hatha K.S Christi Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags Revista de Biología Tropical Suelo de manglar bacteria heterotrófica contaminación plástica degradación microbiana biodegradación enzimas hidrolíticas Mangrove soil heterotrophic bacteria plastic pollution microbial degradation biodegradation hydrolytic enzymes |
| title | Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| title_full | Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| title_fullStr | Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| title_full_unstemmed | Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| title_short | Diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| title_sort | diversity and effectiveness of tropical mangrove soil microflora on the degradation of polythene carry bags |
| topic | Suelo de manglar bacteria heterotrófica contaminación plástica degradación microbiana biodegradación enzimas hidrolíticas Mangrove soil heterotrophic bacteria plastic pollution microbial degradation biodegradation hydrolytic enzymes |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442007000300004 |
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