Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report
Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/11/2/98 |
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| author | Barbara Sterczała Kinga Grzech-Leśniak Olga Michel Witold Trzeciakowski Marzena Dominiak Kamil Jurczyszyn |
| author_facet | Barbara Sterczała Kinga Grzech-Leśniak Olga Michel Witold Trzeciakowski Marzena Dominiak Kamil Jurczyszyn |
| author_sort | Barbara Sterczała |
| collection | DOAJ |
| description | Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser (980 nm), and prototype led laser emitting 405, 450, and 635 nm were used to irradiate the fibroblasts. Due to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm<sup>2</sup>, 25 J/cm<sup>2</sup>, 64 J/cm<sup>2</sup>. The viability and proliferation of cells were determined using the (Thiazolyl Blue Tetrazolium Blue) (MTT) test conducted 24, 48, and 72 h after laser irradiation. Results: The highest percentage of mitochondrial activity (MA = 122.1%) was observed in the group irradiated with the 635 nm laser, with an energy density of 64 J/cm<sup>2</sup> after 48 h. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405 nm laser at 25 J/cm<sup>2</sup> gave similar results to the 635 nm laser. Conclusions: The application of the 635 nm and 405 nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts. |
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| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-09T05:49:10Z |
| publishDate | 2021-02-01 |
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| series | Journal of Personalized Medicine |
| spelling | doaj.art-71b4a32b323842bfa2626e0725303e8f2023-12-03T12:18:59ZengMDPI AGJournal of Personalized Medicine2075-44262021-02-011129810.3390/jpm11020098Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary ReportBarbara Sterczała0Kinga Grzech-Leśniak1Olga Michel2Witold Trzeciakowski3Marzena Dominiak4Kamil Jurczyszyn5Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, PolandLaser Laboratory at Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, PolandDepartment of Molecular and Cell Biology, Wroclaw Medical University, 50-556 Wroclaw, PolandInstitute of High Pressure Physics, Polish Academy of Sciences, 01-142 Warsaw, PolandDental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, PolandDental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, PolandPurpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser (980 nm), and prototype led laser emitting 405, 450, and 635 nm were used to irradiate the fibroblasts. Due to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm<sup>2</sup>, 25 J/cm<sup>2</sup>, 64 J/cm<sup>2</sup>. The viability and proliferation of cells were determined using the (Thiazolyl Blue Tetrazolium Blue) (MTT) test conducted 24, 48, and 72 h after laser irradiation. Results: The highest percentage of mitochondrial activity (MA = 122.1%) was observed in the group irradiated with the 635 nm laser, with an energy density of 64 J/cm<sup>2</sup> after 48 h. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405 nm laser at 25 J/cm<sup>2</sup> gave similar results to the 635 nm laser. Conclusions: The application of the 635 nm and 405 nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.https://www.mdpi.com/2075-4426/11/2/98cell proliferationhuman gingival fibroblastsphotobiomodulationPBMsoft tissue regeneration |
| spellingShingle | Barbara Sterczała Kinga Grzech-Leśniak Olga Michel Witold Trzeciakowski Marzena Dominiak Kamil Jurczyszyn Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report Journal of Personalized Medicine cell proliferation human gingival fibroblasts photobiomodulation PBM soft tissue regeneration |
| title | Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report |
| title_full | Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report |
| title_fullStr | Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report |
| title_full_unstemmed | Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report |
| title_short | Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report |
| title_sort | assessment of human gingival fibroblast proliferation after laser stimulation in vitro using different laser types and wavelengths 1064 980 635 450 and 405 nm preliminary report |
| topic | cell proliferation human gingival fibroblasts photobiomodulation PBM soft tissue regeneration |
| url | https://www.mdpi.com/2075-4426/11/2/98 |
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