<i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps
Heavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation. <i>Trifolium repens</i>, a heavy metal excluder,...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/1002 |
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| author | Ewa Oleńska Valeria Imperato Wanda Małek Tadeusz Włostowski Małgorzata Wójcik Izabela Swiecicka Jaco Vangronsveld Sofie Thijs |
| author_facet | Ewa Oleńska Valeria Imperato Wanda Małek Tadeusz Włostowski Małgorzata Wójcik Izabela Swiecicka Jaco Vangronsveld Sofie Thijs |
| author_sort | Ewa Oleńska |
| collection | DOAJ |
| description | Heavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation. <i>Trifolium repens</i>, a heavy metal excluder, naturally occurs on metal mine waste heaps in southern Poland characterized by high total metal concentrations. The purpose of the present study was to assess the effects of toxic metals on the diversity and metabolic properties of the microbial communities in rhizospheric soil and vegetative tissues of <i>T. repens</i> growing on three 70–100-years old Zn–Pb mine waste heaps in comparison to <i>Trifolium</i>-associated bacteria from a non-polluted reference site. In total, 113 cultivable strains were isolated and used for 16S rRNA gene Sanger sequencing in order to determine their genetic affiliation and for in vitro testing of their plant growth promotion traits. Taxa richness and phenotypic diversity in communities of metalliferous origin were significantly lower (<i>p</i> < 0.0001) compared to those from the reference site. Two strains, <i>Bacillus megaterium</i> BolR EW3_A03 and <i>Stenotrophomonas maltophilia</i> BolN EW3_B03, isolated from a Zn–Pb mine waste heap which tested positive for all examined plant growth promoting traits and which showed co-tolerance to Zn, Cu, Cd, and Pb can be considered as potential facilitators of phytostabilization. |
| first_indexed | 2024-03-10T17:50:55Z |
| format | Article |
| id | doaj.art-71b4ea5facef42e1810d6be796c4a277 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T17:50:55Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-71b4ea5facef42e1810d6be796c4a2772023-11-20T09:21:35ZengMDPI AGPlants2223-77472020-08-0198100210.3390/plants9081002<i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste HeapsEwa Oleńska0Valeria Imperato1Wanda Małek2Tadeusz Włostowski3Małgorzata Wójcik4Izabela Swiecicka5Jaco Vangronsveld6Sofie Thijs7Department of Microbiology and Biotechnology, Faculty of Biology, University of Białystok, Ciołkowskiego 1J, 15-245 Białystok, PolandFaculty of Sciences, Centre for Environmental Sciences, Hasselt University, Agoralaan D, B-3590 Diepenbeek, BelgiumDepartment of Genetics and Microbiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Evolutional and Physiological Ecology, Faculty of Biology, University of Białystok, Ciołkowskiego 1J, 15-245 Białystok, PolandDepartment of Plant Physiology and Biophysics, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biology, University of Białystok, Ciołkowskiego 1J, 15-245 Białystok, PolandFaculty of Sciences, Centre for Environmental Sciences, Hasselt University, Agoralaan D, B-3590 Diepenbeek, BelgiumFaculty of Sciences, Centre for Environmental Sciences, Hasselt University, Agoralaan D, B-3590 Diepenbeek, BelgiumHeavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation. <i>Trifolium repens</i>, a heavy metal excluder, naturally occurs on metal mine waste heaps in southern Poland characterized by high total metal concentrations. The purpose of the present study was to assess the effects of toxic metals on the diversity and metabolic properties of the microbial communities in rhizospheric soil and vegetative tissues of <i>T. repens</i> growing on three 70–100-years old Zn–Pb mine waste heaps in comparison to <i>Trifolium</i>-associated bacteria from a non-polluted reference site. In total, 113 cultivable strains were isolated and used for 16S rRNA gene Sanger sequencing in order to determine their genetic affiliation and for in vitro testing of their plant growth promotion traits. Taxa richness and phenotypic diversity in communities of metalliferous origin were significantly lower (<i>p</i> < 0.0001) compared to those from the reference site. Two strains, <i>Bacillus megaterium</i> BolR EW3_A03 and <i>Stenotrophomonas maltophilia</i> BolN EW3_B03, isolated from a Zn–Pb mine waste heap which tested positive for all examined plant growth promoting traits and which showed co-tolerance to Zn, Cu, Cd, and Pb can be considered as potential facilitators of phytostabilization.https://www.mdpi.com/2223-7747/9/8/1002<i>Trifolium repens</i>bacterial endophytesmetalsplant growth promotionphytoremediation16S rRNA gene |
| spellingShingle | Ewa Oleńska Valeria Imperato Wanda Małek Tadeusz Włostowski Małgorzata Wójcik Izabela Swiecicka Jaco Vangronsveld Sofie Thijs <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps Plants <i>Trifolium repens</i> bacterial endophytes metals plant growth promotion phytoremediation 16S rRNA gene |
| title | <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps |
| title_full | <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps |
| title_fullStr | <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps |
| title_full_unstemmed | <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps |
| title_short | <i>Trifolium repens</i>-Associated Bacteria as a Potential Tool to Facilitate Phytostabilization of Zinc and Lead Polluted Waste Heaps |
| title_sort | i trifolium repens i associated bacteria as a potential tool to facilitate phytostabilization of zinc and lead polluted waste heaps |
| topic | <i>Trifolium repens</i> bacterial endophytes metals plant growth promotion phytoremediation 16S rRNA gene |
| url | https://www.mdpi.com/2223-7747/9/8/1002 |
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