Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking
Objective Osteosarcoma is a primary malignancy originating from mesenchymal tissue characterized by rapid growth, early metastasis and poor prognosis. Ginsenoside Rg5 (G‐Rg5) is a minor ginsenoside extracted from Panax ginseng C.A. Meyer which has been discovered to possess anti‐tumor properties. Th...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
|
| Series: | Orthopaedic Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/os.13971 |
| _version_ | 1797335257254461440 |
|---|---|
| author | Ming‐yang Liu Dong‐xin Jiang Xiang Zhao Liang Zhang Yu Zhang Zhen‐dong Liu Run‐ze Liu Hai‐jun Li Xiao‐yu Rong Yan‐zheng Gao |
| author_facet | Ming‐yang Liu Dong‐xin Jiang Xiang Zhao Liang Zhang Yu Zhang Zhen‐dong Liu Run‐ze Liu Hai‐jun Li Xiao‐yu Rong Yan‐zheng Gao |
| author_sort | Ming‐yang Liu |
| collection | DOAJ |
| description | Objective Osteosarcoma is a primary malignancy originating from mesenchymal tissue characterized by rapid growth, early metastasis and poor prognosis. Ginsenoside Rg5 (G‐Rg5) is a minor ginsenoside extracted from Panax ginseng C.A. Meyer which has been discovered to possess anti‐tumor properties. The objective of current study was to explore the mechanism of G‐Rg5 in the treatment of osteosarcoma by network pharmacology and molecular docking technology. Methods Pharmmapper, SwissTargetPrediction and similarity ensemble approach databases were used to obtain the pharmacological targets of G‐Rg5. Related genes of osteosarcoma were searched for in the GeneCards, OMIM and DrugBank databases. The targets of G‐Rg5 and the related genes of osteosarcoma were intersected to obtain the potential target genes of G‐Rg5 in the treatment of osteosarccoma. The STRING database and Cytoscape 3.8.2 software were used to construct the protein–protein interaction (PPI) network, and the Database for Annotation, Visualization and Integrated Discovery (DAVID) platform was used to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. AutoDock vina software was used to perform molecular docking between G‐Rg5 and hub targets. The hub genes were imported into the Kaplan–Meier Plotter online database for survival analysis. Results A total of 61 overlapping targets were obtained. The related signaling pathways mainly included PI3K‐Akt signaling pathway, Proteoglycans in cancer, Lipid and atherosclerosis and Kaposi sarcoma‐associated herpesvirus infection. Six hub targets including PIK3CA, SRC, TP53, MAPK1, EGFR, and VEGFA were obtained through PPI network and targets‐pathways network analyses. The results of molecular docking showed that the binding energies were all less than –7 kcal/mol. And the results of survival analysis showed TP53 and VEGFA affect the prognosis of sarcoma patients. Conclusion This study explored the possible mechanism of G‐Rg5 in the treatment of osteosarcoma using network pharmacology method, suggesting that G‐Rg5 has the characteristics of multi‐targets and multi‐pathways in the treatment of osteosarcoma, which lays a foundation for the follow‐up experimental and clinical researches on the therapeutic effects of G‐Rg5 on osteosarcoma. |
| first_indexed | 2024-03-08T08:34:20Z |
| format | Article |
| id | doaj.art-71b74e61ff034dddbd9d3622e832563b |
| institution | Directory Open Access Journal |
| issn | 1757-7853 1757-7861 |
| language | English |
| last_indexed | 2024-03-08T08:34:20Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Orthopaedic Surgery |
| spelling | doaj.art-71b74e61ff034dddbd9d3622e832563b2024-02-02T01:54:28ZengWileyOrthopaedic Surgery1757-78531757-78612024-02-0116246247010.1111/os.13971Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular DockingMing‐yang Liu0Dong‐xin Jiang1Xiang Zhao2Liang Zhang3Yu Zhang4Zhen‐dong Liu5Run‐ze Liu6Hai‐jun Li7Xiao‐yu Rong8Yan‐zheng Gao9Henan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaDepartment of Immunity, Institute of Translational Medicine The First Hospital of Jilin University Jilin ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaHenan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University People's Hospital of Henan University Zhengzhou ChinaObjective Osteosarcoma is a primary malignancy originating from mesenchymal tissue characterized by rapid growth, early metastasis and poor prognosis. Ginsenoside Rg5 (G‐Rg5) is a minor ginsenoside extracted from Panax ginseng C.A. Meyer which has been discovered to possess anti‐tumor properties. The objective of current study was to explore the mechanism of G‐Rg5 in the treatment of osteosarcoma by network pharmacology and molecular docking technology. Methods Pharmmapper, SwissTargetPrediction and similarity ensemble approach databases were used to obtain the pharmacological targets of G‐Rg5. Related genes of osteosarcoma were searched for in the GeneCards, OMIM and DrugBank databases. The targets of G‐Rg5 and the related genes of osteosarcoma were intersected to obtain the potential target genes of G‐Rg5 in the treatment of osteosarccoma. The STRING database and Cytoscape 3.8.2 software were used to construct the protein–protein interaction (PPI) network, and the Database for Annotation, Visualization and Integrated Discovery (DAVID) platform was used to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. AutoDock vina software was used to perform molecular docking between G‐Rg5 and hub targets. The hub genes were imported into the Kaplan–Meier Plotter online database for survival analysis. Results A total of 61 overlapping targets were obtained. The related signaling pathways mainly included PI3K‐Akt signaling pathway, Proteoglycans in cancer, Lipid and atherosclerosis and Kaposi sarcoma‐associated herpesvirus infection. Six hub targets including PIK3CA, SRC, TP53, MAPK1, EGFR, and VEGFA were obtained through PPI network and targets‐pathways network analyses. The results of molecular docking showed that the binding energies were all less than –7 kcal/mol. And the results of survival analysis showed TP53 and VEGFA affect the prognosis of sarcoma patients. Conclusion This study explored the possible mechanism of G‐Rg5 in the treatment of osteosarcoma using network pharmacology method, suggesting that G‐Rg5 has the characteristics of multi‐targets and multi‐pathways in the treatment of osteosarcoma, which lays a foundation for the follow‐up experimental and clinical researches on the therapeutic effects of G‐Rg5 on osteosarcoma.https://doi.org/10.1111/os.13971Ginsenoside Rg5Molecular dockingNetwork pharmacologyOsteosarcomaSurvival analysis |
| spellingShingle | Ming‐yang Liu Dong‐xin Jiang Xiang Zhao Liang Zhang Yu Zhang Zhen‐dong Liu Run‐ze Liu Hai‐jun Li Xiao‐yu Rong Yan‐zheng Gao Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking Orthopaedic Surgery Ginsenoside Rg5 Molecular docking Network pharmacology Osteosarcoma Survival analysis |
| title | Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking |
| title_full | Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking |
| title_fullStr | Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking |
| title_full_unstemmed | Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking |
| title_short | Exploration in the Mechanism of Ginsenoside Rg5 for the Treatment of Osteosarcoma by Network Pharmacology and Molecular Docking |
| title_sort | exploration in the mechanism of ginsenoside rg5 for the treatment of osteosarcoma by network pharmacology and molecular docking |
| topic | Ginsenoside Rg5 Molecular docking Network pharmacology Osteosarcoma Survival analysis |
| url | https://doi.org/10.1111/os.13971 |
| work_keys_str_mv | AT mingyangliu explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT dongxinjiang explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT xiangzhao explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT liangzhang explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT yuzhang explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT zhendongliu explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT runzeliu explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT haijunli explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT xiaoyurong explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking AT yanzhenggao explorationinthemechanismofginsenosiderg5forthetreatmentofosteosarcomabynetworkpharmacologyandmoleculardocking |