Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia
This study determined the optimal conditions for the deep eutectic solvent (DES) pretreatment of sugarcane leaves and the best fermentation mode for hydrogen and methane production from DES-pretreated sugarcane leaves. Choline chloride (ChCl):monoethanolamine (MEA) is the most effective solvent for...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/8/396 |
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| author | Apik Khautsart Miftah Sureewan Sittijunda Tsuyoshi Imai Apilak Salakkam Alissara Reungsang |
| author_facet | Apik Khautsart Miftah Sureewan Sittijunda Tsuyoshi Imai Apilak Salakkam Alissara Reungsang |
| author_sort | Apik Khautsart Miftah |
| collection | DOAJ |
| description | This study determined the optimal conditions for the deep eutectic solvent (DES) pretreatment of sugarcane leaves and the best fermentation mode for hydrogen and methane production from DES-pretreated sugarcane leaves. Choline chloride (ChCl):monoethanolamine (MEA) is the most effective solvent for removing lignin from sugarcane leaves. The optimum conditions were a ChCl: MEA molar ratio of 1:6, 120 °C, 3 h, and substrate-to-DES solution ratio of 1:12. Under these conditions, 86.37 ± 0.36% lignin removal and 73.98 ± 0.42% hemicellulose removal were achieved, whereas 84.13 ± 0.77% cellulose was recovered. At a substrate loading of 4 g volatile solids (VS), the simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF) processes yielded maximum hydrogen productions of 3187 ± 202 and 2135 ± 315 mL H<sub>2</sub>/L, respectively. In the second stage, methane was produced using the hydrogenic effluent. SSF produced 5923 ± 251 mL CH<sub>4</sub>/L, whereas SHF produced 3583 ± 128 mL CH<sub>4</sub>/L. In a one-stage methane production process, a maximum methane production of 4067 ± 320 mL CH<sub>4</sub>/L with a substrate loading of 4 g VS was achieved from the SSF process. SSF proved to be more efficient than SHF for producing hydrogen from DES-pretreated sugarcane leaves in a two-stage hydrogen and methane production process as well as a one-stage methane production process. |
| first_indexed | 2024-03-09T09:57:23Z |
| format | Article |
| id | doaj.art-71b7c8300a634670b98a0370cd0567f4 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T09:57:23Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-71b7c8300a634670b98a0370cd0567f42023-12-01T23:40:57ZengMDPI AGFermentation2311-56372022-08-018839610.3390/fermentation8080396Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic ConsortiaApik Khautsart Miftah0Sureewan Sittijunda1Tsuyoshi Imai2Apilak Salakkam3Alissara Reungsang4Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Environment and Resource Studies, Mahidol University, Nakhon Pathom 73170, ThailandGraduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 755-8611, JapanDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandThis study determined the optimal conditions for the deep eutectic solvent (DES) pretreatment of sugarcane leaves and the best fermentation mode for hydrogen and methane production from DES-pretreated sugarcane leaves. Choline chloride (ChCl):monoethanolamine (MEA) is the most effective solvent for removing lignin from sugarcane leaves. The optimum conditions were a ChCl: MEA molar ratio of 1:6, 120 °C, 3 h, and substrate-to-DES solution ratio of 1:12. Under these conditions, 86.37 ± 0.36% lignin removal and 73.98 ± 0.42% hemicellulose removal were achieved, whereas 84.13 ± 0.77% cellulose was recovered. At a substrate loading of 4 g volatile solids (VS), the simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF) processes yielded maximum hydrogen productions of 3187 ± 202 and 2135 ± 315 mL H<sub>2</sub>/L, respectively. In the second stage, methane was produced using the hydrogenic effluent. SSF produced 5923 ± 251 mL CH<sub>4</sub>/L, whereas SHF produced 3583 ± 128 mL CH<sub>4</sub>/L. In a one-stage methane production process, a maximum methane production of 4067 ± 320 mL CH<sub>4</sub>/L with a substrate loading of 4 g VS was achieved from the SSF process. SSF proved to be more efficient than SHF for producing hydrogen from DES-pretreated sugarcane leaves in a two-stage hydrogen and methane production process as well as a one-stage methane production process.https://www.mdpi.com/2311-5637/8/8/396ionic liquidanaerobic digestionlignocellulosic biomasspretreatmentclean energy |
| spellingShingle | Apik Khautsart Miftah Sureewan Sittijunda Tsuyoshi Imai Apilak Salakkam Alissara Reungsang Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia Fermentation ionic liquid anaerobic digestion lignocellulosic biomass pretreatment clean energy |
| title | Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia |
| title_full | Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia |
| title_fullStr | Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia |
| title_full_unstemmed | Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia |
| title_short | Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia |
| title_sort | biohydrogen and methane production from sugarcane leaves pretreated by deep eutectic solvents and enzymatic hydrolysis by cellulolytic consortia |
| topic | ionic liquid anaerobic digestion lignocellulosic biomass pretreatment clean energy |
| url | https://www.mdpi.com/2311-5637/8/8/396 |
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