Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.
The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0261417 |
| _version_ | 1818752761768968192 |
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| author | R C M Vulders R C van Hoogenhuizen E van der Giessen P J van der Zaag |
| author_facet | R C M Vulders R C van Hoogenhuizen E van der Giessen P J van der Zaag |
| author_sort | R C M Vulders |
| collection | DOAJ |
| description | The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this. |
| first_indexed | 2024-12-18T04:56:36Z |
| format | Article |
| id | doaj.art-71b969c40c1b488f889299480918883e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T04:56:36Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-71b969c40c1b488f889299480918883e2022-12-21T21:20:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011612e026141710.1371/journal.pone.0261417Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.R C M VuldersR C van HoogenhuizenE van der GiessenP J van der ZaagThe use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.https://doi.org/10.1371/journal.pone.0261417 |
| spellingShingle | R C M Vulders R C van Hoogenhuizen E van der Giessen P J van der Zaag Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. PLoS ONE |
| title | Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. |
| title_full | Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. |
| title_fullStr | Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. |
| title_full_unstemmed | Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. |
| title_short | Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect. |
| title_sort | clearing induced tisssue shrinkage a novel observation of a thickness size effect |
| url | https://doi.org/10.1371/journal.pone.0261417 |
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