GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS
Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Romanian Society of Oral Rehabilitation
2020-12-01
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| Series: | Romanian Journal of Oral Rehabilitation |
| Subjects: | |
| Online Access: | http://www.rjor.ro/gingival-fibroblasts-and-nak-1-activated-pro-b-lymphocytes-apoptosis/ |
| Summary: | Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system. |
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| ISSN: | 2066-7000 2601-4661 |