GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS
Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when...
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| Format: | Article |
| Language: | English |
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Romanian Society of Oral Rehabilitation
2020-12-01
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| Series: | Romanian Journal of Oral Rehabilitation |
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| Online Access: | http://www.rjor.ro/gingival-fibroblasts-and-nak-1-activated-pro-b-lymphocytes-apoptosis/ |
| _version_ | 1819297204714602496 |
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| author | Carmen Gentimir (Amititeloaie) Anda Cătălina Zaharia Eugen Bitere Dragoş Acatrinei Ionuţ Cazan Marcel Costuleanu Otilia Boişteanu |
| author_facet | Carmen Gentimir (Amititeloaie) Anda Cătălina Zaharia Eugen Bitere Dragoş Acatrinei Ionuţ Cazan Marcel Costuleanu Otilia Boişteanu |
| author_sort | Carmen Gentimir (Amititeloaie) |
| collection | DOAJ |
| description | Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system. |
| first_indexed | 2024-12-24T05:10:18Z |
| format | Article |
| id | doaj.art-71b9813f9d07496794c7fc5a14f07301 |
| institution | Directory Open Access Journal |
| issn | 2066-7000 2601-4661 |
| language | English |
| last_indexed | 2024-12-24T05:10:18Z |
| publishDate | 2020-12-01 |
| publisher | Romanian Society of Oral Rehabilitation |
| record_format | Article |
| series | Romanian Journal of Oral Rehabilitation |
| spelling | doaj.art-71b9813f9d07496794c7fc5a14f073012022-12-21T17:13:42ZengRomanian Society of Oral RehabilitationRomanian Journal of Oral Rehabilitation2066-70002601-46612020-12-011245156GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSISCarmen Gentimir (Amititeloaie)0Anda Cătălina Zaharia1Eugen Bitere2Dragoş Acatrinei3Ionuţ Cazan4Marcel Costuleanu5Otilia Boişteanu6Grigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryGrigore T. Popa" University of Medicine and Pharmacy - Iași, Romania, Faculty of Dental Medicine, Department of Oral and Maxillo-Facial SurgeryAim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system.http://www.rjor.ro/gingival-fibroblasts-and-nak-1-activated-pro-b-lymphocytes-apoptosis/apoptosisautophagygingival fibroblastnak-1pro-b lymphocyte |
| spellingShingle | Carmen Gentimir (Amititeloaie) Anda Cătălina Zaharia Eugen Bitere Dragoş Acatrinei Ionuţ Cazan Marcel Costuleanu Otilia Boişteanu GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS Romanian Journal of Oral Rehabilitation apoptosis autophagy gingival fibroblast nak-1 pro-b lymphocyte |
| title | GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS |
| title_full | GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS |
| title_fullStr | GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS |
| title_full_unstemmed | GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS |
| title_short | GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS |
| title_sort | gingival fibroblasts and nak 1 activated pro b lymphocytes apoptosis |
| topic | apoptosis autophagy gingival fibroblast nak-1 pro-b lymphocyte |
| url | http://www.rjor.ro/gingival-fibroblasts-and-nak-1-activated-pro-b-lymphocytes-apoptosis/ |
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