Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i>
<i>Dendrobium</i> <i>catenatum</i>, a valuable Chinese herb, frequently experiences abiotic stresses, such as cold and drought, under natural conditions. Nonphosphorus glycerolipid synthase (NGLS) genes are closely linked to the homeostasis of membrane lipids under abiotic st...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1204 |
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| author | Xinqiao Zhan Yichun Qian Bizeng Mao |
| author_facet | Xinqiao Zhan Yichun Qian Bizeng Mao |
| author_sort | Xinqiao Zhan |
| collection | DOAJ |
| description | <i>Dendrobium</i> <i>catenatum</i>, a valuable Chinese herb, frequently experiences abiotic stresses, such as cold and drought, under natural conditions. Nonphosphorus glycerolipid synthase (NGLS) genes are closely linked to the homeostasis of membrane lipids under abiotic stress in plants. However, there is limited information on <i>NGLS</i> genes in <i>D</i>. <i>catenatum</i>. In this study, a total of eight <i>DcaNGLS</i> genes were identified from the <i>D</i>. <i>catenatum</i> genome; these included three <i>monogalactosyldiacylglycerol synthase</i> (<i>DcaMGD1</i>, <i>2</i>, <i>3</i>) genes, two <i>digalactosyldiacylglycerol synthase</i> (<i>DcaDGD1</i>, <i>2</i>) genes, and three <i>sulfoquinovosyldiacylglycerol synthase</i> (<i>DcaSQD1</i>, <i>2.1</i>, <i>2.2</i>) genes. The gene structures and conserved motifs in the <i>DcaNGLSs</i> showed a high conservation during their evolution. Gene expression profiling showed that the <i>DcaNGLSs</i> were highly expressed in specific tissues and during rapid growth stages. Furthermore, most <i>DcaNGLSs</i> were strongly induced by freezing and post-freezing recovery. <i>DcaMGD1</i> and <i>DcaSQDs</i> were greatly induced by salt stress in leaves, while <i>DcaDGDs</i> were primarily induced by salt stress in roots. Under drought stress, most <i>DcaNGLSs</i> were regulated by circadian rhythms, and <i>DcaSQD2</i> was closely associated with drought recovery. Transcriptome analysis also revealed that MYB might be regulated by circadian rhythm and co-expressed with <i>DcaNGLSs</i> under drought stress. These results provide insight for the further functional investigation of NGLS and the regulation of nonphosphorus glycerolipid biosynthesis in <i>Dendrobium</i>. |
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| institution | Directory Open Access Journal |
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| spelling | doaj.art-71b9b70dde284f5a96f35fdfe6ff0a162023-11-21T23:57:33ZengMDPI AGPlants2223-77472021-06-01106120410.3390/plants10061204Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i>Xinqiao Zhan0Yichun Qian1Bizeng Mao2Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, ChinaInstitute of Biotechnology, Zhejiang University, Hangzhou 310000, ChinaInstitute of Biotechnology, Zhejiang University, Hangzhou 310000, China<i>Dendrobium</i> <i>catenatum</i>, a valuable Chinese herb, frequently experiences abiotic stresses, such as cold and drought, under natural conditions. Nonphosphorus glycerolipid synthase (NGLS) genes are closely linked to the homeostasis of membrane lipids under abiotic stress in plants. However, there is limited information on <i>NGLS</i> genes in <i>D</i>. <i>catenatum</i>. In this study, a total of eight <i>DcaNGLS</i> genes were identified from the <i>D</i>. <i>catenatum</i> genome; these included three <i>monogalactosyldiacylglycerol synthase</i> (<i>DcaMGD1</i>, <i>2</i>, <i>3</i>) genes, two <i>digalactosyldiacylglycerol synthase</i> (<i>DcaDGD1</i>, <i>2</i>) genes, and three <i>sulfoquinovosyldiacylglycerol synthase</i> (<i>DcaSQD1</i>, <i>2.1</i>, <i>2.2</i>) genes. The gene structures and conserved motifs in the <i>DcaNGLSs</i> showed a high conservation during their evolution. Gene expression profiling showed that the <i>DcaNGLSs</i> were highly expressed in specific tissues and during rapid growth stages. Furthermore, most <i>DcaNGLSs</i> were strongly induced by freezing and post-freezing recovery. <i>DcaMGD1</i> and <i>DcaSQDs</i> were greatly induced by salt stress in leaves, while <i>DcaDGDs</i> were primarily induced by salt stress in roots. Under drought stress, most <i>DcaNGLSs</i> were regulated by circadian rhythms, and <i>DcaSQD2</i> was closely associated with drought recovery. Transcriptome analysis also revealed that MYB might be regulated by circadian rhythm and co-expressed with <i>DcaNGLSs</i> under drought stress. These results provide insight for the further functional investigation of NGLS and the regulation of nonphosphorus glycerolipid biosynthesis in <i>Dendrobium</i>.https://www.mdpi.com/2223-7747/10/6/1204nonphosphorus glycerolipid synthaseabiotic stresscircadian regulation<i>Dendrobium</i> |
| spellingShingle | Xinqiao Zhan Yichun Qian Bizeng Mao Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> Plants nonphosphorus glycerolipid synthase abiotic stress circadian regulation <i>Dendrobium</i> |
| title | Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> |
| title_full | Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> |
| title_fullStr | Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> |
| title_full_unstemmed | Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> |
| title_short | Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in <i>Dendrobium catenatum</i> |
| title_sort | identification and expression profiling of nonphosphorus glycerolipid synthase genes in response to abiotic stresses in i dendrobium catenatum i |
| topic | nonphosphorus glycerolipid synthase abiotic stress circadian regulation <i>Dendrobium</i> |
| url | https://www.mdpi.com/2223-7747/10/6/1204 |
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