An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials
<p>Abstract</p> <p>Background</p> <p>Following exposure via inhalation, intratracheal instillation or ingestion some nanomaterials (NM) have been shown to translocate to the liver. Since oxidative stress has been implicated as a possible mechanism for NM toxicity this s...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | http://www.particleandfibretoxicology.com/content/9/1/28 |
| _version_ | 1811252819264536576 |
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| author | Kermanizadeh Ali Gaiser Birgit K Hutchison Gary R Stone Vicki |
| author_facet | Kermanizadeh Ali Gaiser Birgit K Hutchison Gary R Stone Vicki |
| author_sort | Kermanizadeh Ali |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Following exposure via inhalation, intratracheal instillation or ingestion some nanomaterials (NM) have been shown to translocate to the liver. Since oxidative stress has been implicated as a possible mechanism for NM toxicity this study aimed to investigate the effects of various materials (five titanium dioxide (TiO<sub>2</sub>), two zinc oxide (ZnO), two multi-walled carbon nanotubes (MWCNT) and one silver (Ag) NM) on oxidative responses of C3A cell line as a model for potential detrimental properties of nanomaterials on the liver.</p> <p>Results</p> <p>We noted a dose dependant decrease in the cellular glutathione content following exposure of the C3A cells to Ag, the ZnO and the MWCNTs. Intracellular ROS levels were also measured and shown to increase significantly following exposure of the C3A to the low toxicity NMs (MWCNT and TiO<sub>2</sub>). The antioxidant Trolox in part prevented the detrimental effect of NMs on cell viability, and decreased the NM induced IL8 production after exposure to all but the Ag particulate. Following 4 hr exposure of the C3A cells to sub-lethal levels of the NMs, the largest amount of DNA damage was induced by two of the TiO<sub>2</sub> samples (7 nm and the positively charged 10 nm particles).</p> <p>Conclusions</p> <p>All ten NMs exhibited effects on the hepatocyte cell line that were at least in part ROS/oxidative stress mediated. These effects included mild genotoxicity and IL8 production for all NM except the Ag possibly due to its highly cytotoxic nature.</p> |
| first_indexed | 2024-04-12T16:40:30Z |
| format | Article |
| id | doaj.art-71bb2eb51f014b628807d2d7bed0dcc3 |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-04-12T16:40:30Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-71bb2eb51f014b628807d2d7bed0dcc32022-12-22T03:24:48ZengBMCParticle and Fibre Toxicology1743-89772012-07-01912810.1186/1743-8977-9-28An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterialsKermanizadeh AliGaiser Birgit KHutchison Gary RStone Vicki<p>Abstract</p> <p>Background</p> <p>Following exposure via inhalation, intratracheal instillation or ingestion some nanomaterials (NM) have been shown to translocate to the liver. Since oxidative stress has been implicated as a possible mechanism for NM toxicity this study aimed to investigate the effects of various materials (five titanium dioxide (TiO<sub>2</sub>), two zinc oxide (ZnO), two multi-walled carbon nanotubes (MWCNT) and one silver (Ag) NM) on oxidative responses of C3A cell line as a model for potential detrimental properties of nanomaterials on the liver.</p> <p>Results</p> <p>We noted a dose dependant decrease in the cellular glutathione content following exposure of the C3A cells to Ag, the ZnO and the MWCNTs. Intracellular ROS levels were also measured and shown to increase significantly following exposure of the C3A to the low toxicity NMs (MWCNT and TiO<sub>2</sub>). The antioxidant Trolox in part prevented the detrimental effect of NMs on cell viability, and decreased the NM induced IL8 production after exposure to all but the Ag particulate. Following 4 hr exposure of the C3A cells to sub-lethal levels of the NMs, the largest amount of DNA damage was induced by two of the TiO<sub>2</sub> samples (7 nm and the positively charged 10 nm particles).</p> <p>Conclusions</p> <p>All ten NMs exhibited effects on the hepatocyte cell line that were at least in part ROS/oxidative stress mediated. These effects included mild genotoxicity and IL8 production for all NM except the Ag possibly due to its highly cytotoxic nature.</p>http://www.particleandfibretoxicology.com/content/9/1/28LiverNanomaterialsOxidative stressAntioxidantGenotoxicity |
| spellingShingle | Kermanizadeh Ali Gaiser Birgit K Hutchison Gary R Stone Vicki An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials Particle and Fibre Toxicology Liver Nanomaterials Oxidative stress Antioxidant Genotoxicity |
| title | An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| title_full | An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| title_fullStr | An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| title_full_unstemmed | An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| title_short | An <it>in vitro</it> liver model - assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| title_sort | it in vitro it liver model assessing oxidative stress and genotoxicity following exposure of hepatocytes to a panel of engineered nanomaterials |
| topic | Liver Nanomaterials Oxidative stress Antioxidant Genotoxicity |
| url | http://www.particleandfibretoxicology.com/content/9/1/28 |
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