A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity
The microbes in the rhizosphere (or mycorrhizosphere) could promote plant growth, however, it is unclear whether mycorrhizosphere microbes could fight multiple fungal pathogens. In this study, twenty-one bacterial strains distributed in 6 genera, including 5 Pseudomonas strains, were isolated from m...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.954160/full |
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| author | Pengjie Han Pengjie Han Tianrui Liu Yuan Zheng Ruiqi Song Tiegui Nan Xiaolong Yang Luqi Huang Luqi Huang Yuan Yuan |
| author_facet | Pengjie Han Pengjie Han Tianrui Liu Yuan Zheng Ruiqi Song Tiegui Nan Xiaolong Yang Luqi Huang Luqi Huang Yuan Yuan |
| author_sort | Pengjie Han |
| collection | DOAJ |
| description | The microbes in the rhizosphere (or mycorrhizosphere) could promote plant growth, however, it is unclear whether mycorrhizosphere microbes could fight multiple fungal pathogens. In this study, twenty-one bacterial strains distributed in 6 genera, including 5 Pseudomonas strains, were isolated from mycorrhizal samples of Polyporus umbellatus that rely on other fungi during their life cycles. Further screening and pot experiments showed that the Pseudomonas strain ZL8 not only inhibited the growth of phytopathogenic fungi, but also promoted the growth of Salvia miltiorrhiza through inhibiting its wilting. In addition, strain ZL8 was found to have the ability to dissolve phosphate, produce IAA and siderophore. Nineteen compounds were identified from the fermentation broth of strain ZL8, of which 2,4-diacetylphloroglucinol (DAPG) had a significant inhibitory effect on phytopathogenic fungi with a minimum inhibitory concentration of 3.12–25 μg/mL. Molecular docking predicted that DAPG could bind to myosin I at two unique sites, which may be responsible to the inhibition of fungal growth. The evaluation results showed that strain ZL8 can be used to develop a dual-purpose biocontrol agents and biofertilizer. These results also provide new insights into the discovery and utilization of new resources for biocontrol agents and biolfertilizers. |
| first_indexed | 2024-12-10T22:46:10Z |
| format | Article |
| id | doaj.art-71bb655ad8e84fcea380216c94d6c81f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T22:46:10Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-71bb655ad8e84fcea380216c94d6c81f2022-12-22T01:30:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.954160954160A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal ActivityPengjie Han0Pengjie Han1Tianrui Liu2Yuan Zheng3Ruiqi Song4Tiegui Nan5Xiaolong Yang6Luqi Huang7Luqi Huang8Yuan Yuan9School of Pharmaceutical Sciences, Peking University, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, ChinaSchool of Pharmaceutical Sciences, Peking University, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaThe microbes in the rhizosphere (or mycorrhizosphere) could promote plant growth, however, it is unclear whether mycorrhizosphere microbes could fight multiple fungal pathogens. In this study, twenty-one bacterial strains distributed in 6 genera, including 5 Pseudomonas strains, were isolated from mycorrhizal samples of Polyporus umbellatus that rely on other fungi during their life cycles. Further screening and pot experiments showed that the Pseudomonas strain ZL8 not only inhibited the growth of phytopathogenic fungi, but also promoted the growth of Salvia miltiorrhiza through inhibiting its wilting. In addition, strain ZL8 was found to have the ability to dissolve phosphate, produce IAA and siderophore. Nineteen compounds were identified from the fermentation broth of strain ZL8, of which 2,4-diacetylphloroglucinol (DAPG) had a significant inhibitory effect on phytopathogenic fungi with a minimum inhibitory concentration of 3.12–25 μg/mL. Molecular docking predicted that DAPG could bind to myosin I at two unique sites, which may be responsible to the inhibition of fungal growth. The evaluation results showed that strain ZL8 can be used to develop a dual-purpose biocontrol agents and biofertilizer. These results also provide new insights into the discovery and utilization of new resources for biocontrol agents and biolfertilizers.https://www.frontiersin.org/articles/10.3389/fpls.2022.954160/fullPGPBPseudomonas spp.biocontrol agentsbiofertilizerFusarium spp.DAPG |
| spellingShingle | Pengjie Han Pengjie Han Tianrui Liu Yuan Zheng Ruiqi Song Tiegui Nan Xiaolong Yang Luqi Huang Luqi Huang Yuan Yuan A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity Frontiers in Plant Science PGPB Pseudomonas spp. biocontrol agents biofertilizer Fusarium spp. DAPG |
| title | A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity |
| title_full | A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity |
| title_fullStr | A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity |
| title_full_unstemmed | A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity |
| title_short | A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity |
| title_sort | mycorrhizal bacteria strain isolated from polyporus umbellatus exhibits broad spectrum antifungal activity |
| topic | PGPB Pseudomonas spp. biocontrol agents biofertilizer Fusarium spp. DAPG |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.954160/full |
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