Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province
This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2021-09-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acve-2021-0022 |
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| author | Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay |
| author_facet | Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay |
| author_sort | Çelik Burçak Aslan |
| collection | DOAJ |
| description | This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province. |
| first_indexed | 2024-04-11T20:24:53Z |
| format | Article |
| id | doaj.art-71bbcb50f27a4832ae8eb48586001776 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-04-11T20:24:53Z |
| publishDate | 2021-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-71bbcb50f27a4832ae8eb485860017762022-12-22T04:04:41ZengSciendoActa Veterinaria1820-74482021-09-0171324725510.2478/acve-2021-0022Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt ProvinceÇelik Burçak Aslan0Ayan Adnan1Yilmaz Ali Bilgin2Çelik Özgür Yaşar3Kilinç Özlem Orunç4Ayan Özge Oktay5Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, TurkeyDepartment of Genetics, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, TurkeySchool of Health, Van Yuzuncu Yil University, Van, TurkeyDepartment of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, TurkeyÖzalp Vocational School, Van Yuzuncu Yil University, Van, TurkeyDepartment of Medical Parasitology, Van Yuzuncu Yil University School of Medicine, Van, TurkeyThis study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.https://doi.org/10.2478/acve-2021-0022ehrlichia canisnested pcrrhipicephalus sanguineussiirtturkey |
| spellingShingle | Çelik Burçak Aslan Ayan Adnan Yilmaz Ali Bilgin Çelik Özgür Yaşar Kilinç Özlem Orunç Ayan Özge Oktay Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province Acta Veterinaria ehrlichia canis nested pcr rhipicephalus sanguineus siirt turkey |
| title | Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
| title_full | Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
| title_fullStr | Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
| title_full_unstemmed | Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
| title_short | Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province |
| title_sort | molecular identification of ehrlichia canis in rhipicephalus sanguineus ticks from siirt province |
| topic | ehrlichia canis nested pcr rhipicephalus sanguineus siirt turkey |
| url | https://doi.org/10.2478/acve-2021-0022 |
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