Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences
Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, su...
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| Format: | Article |
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Elsevier
2020-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037020303275 |
| _version_ | 1818325372033302528 |
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| author | Long Chen Xingye Chen Xusan Yang Chao He Miaoyan Wang Peng Xi Juntao Gao |
| author_facet | Long Chen Xingye Chen Xusan Yang Chao He Miaoyan Wang Peng Xi Juntao Gao |
| author_sort | Long Chen |
| collection | DOAJ |
| description | Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization. |
| first_indexed | 2024-12-13T11:43:26Z |
| format | Article |
| id | doaj.art-71bc2cc2b05d47b88e6e6dd68ad8f892 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-12-13T11:43:26Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-71bc2cc2b05d47b88e6e6dd68ad8f8922022-12-21T23:47:34ZengElsevierComputational and Structural Biotechnology Journal2001-03702020-01-011822092216Advances of super-resolution fluorescence polarization microscopy and its applications in life sciencesLong Chen0Xingye Chen1Xusan Yang2Chao He3Miaoyan Wang4Peng Xi5Juntao Gao6Department of Automation, Tsinghua University, 100084 Beijing, China; MOE Key Laboratory of Bioinformatics; Bioinformatics Division, Center for Synthetic & Systems Biology, BNRist; Center for Synthetic & Systems Biology, Tsinghua University, 100084 Beijing, China; Corresponding authors at: Department of Automation, Tsinghua University, 100084 Beijing, China.Department of Automation, Tsinghua University, 100084 Beijing, ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, ChinaDepartment of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UKDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, ChinaDepartment of Automation, Tsinghua University, 100084 Beijing, China; MOE Key Laboratory of Bioinformatics; Bioinformatics Division, Center for Synthetic & Systems Biology, BNRist; Center for Synthetic & Systems Biology, Tsinghua University, 100084 Beijing, China; Corresponding authors at: Department of Automation, Tsinghua University, 100084 Beijing, China.Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization.http://www.sciencedirect.com/science/article/pii/S2001037020303275Fluorescence polarization microscopyDipole orientationSuper-resolutionBiomolecule interactionsMolecular organization |
| spellingShingle | Long Chen Xingye Chen Xusan Yang Chao He Miaoyan Wang Peng Xi Juntao Gao Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences Computational and Structural Biotechnology Journal Fluorescence polarization microscopy Dipole orientation Super-resolution Biomolecule interactions Molecular organization |
| title | Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences |
| title_full | Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences |
| title_fullStr | Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences |
| title_full_unstemmed | Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences |
| title_short | Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences |
| title_sort | advances of super resolution fluorescence polarization microscopy and its applications in life sciences |
| topic | Fluorescence polarization microscopy Dipole orientation Super-resolution Biomolecule interactions Molecular organization |
| url | http://www.sciencedirect.com/science/article/pii/S2001037020303275 |
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