An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC)
Abstract Small cell lung cancer (SCLC) is a particularly lethal subtype of lung cancer. Metastatic lung tumours lead to most deaths from lung cancer. Predicting and preventing tumour metastasis is crucially essential for patient survivability. Hence, in the current study, we focused on a comprehensi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22252-7 |
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| author | Arsham Mikaeili Namini Motahareh Jahangir Maryam Mohseni Ali Asghar Kolahi Hossein Hassanian-Moghaddam Zeinab Mazloumi Marzieh Motallebi Mojgan Sheikhpour Abolfazl Movafagh |
| author_facet | Arsham Mikaeili Namini Motahareh Jahangir Maryam Mohseni Ali Asghar Kolahi Hossein Hassanian-Moghaddam Zeinab Mazloumi Marzieh Motallebi Mojgan Sheikhpour Abolfazl Movafagh |
| author_sort | Arsham Mikaeili Namini |
| collection | DOAJ |
| description | Abstract Small cell lung cancer (SCLC) is a particularly lethal subtype of lung cancer. Metastatic lung tumours lead to most deaths from lung cancer. Predicting and preventing tumour metastasis is crucially essential for patient survivability. Hence, in the current study, we focused on a comprehensive analysis of lung cancer patients' differentially expressed genes (DEGs) on brain metastasis cell lines. DEGs are analysed through KEGG and GO databases for the most critical biological processes and pathways for enriched DEGs. Additionally, we performed protein–protein interaction (PPI), GeneMANIA, and Kaplan–Meier survival analyses on our DEGs. This article focused on mRNA and lncRNA DEGs for LC patients with brain metastasis and underlying molecular mechanisms. The expression data was gathered from the Gene Expression Omnibus database (GSE161968). We demonstrate that 30 distinct genes are up-expressed in brain metastatic SCLC patients, and 31 genes are down-expressed. All our analyses show that these genes are involved in metastatic SCLC. PPI analysis revealed two hub genes (CAT and APP). The results of this article present three lncRNAs, Including XLOC_l2_000941, LOC100507481, and XLOC_l2_007062, also notable mRNAs, have a close relation with brain metastasis in lung cancer and may have a role in the epithelial-mesenchymal transition (EMT) in tumour cells. |
| first_indexed | 2024-04-12T17:51:01Z |
| format | Article |
| id | doaj.art-71bc3214e11241d490fb312c323f02e1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T17:51:01Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-71bc3214e11241d490fb312c323f02e12022-12-22T03:22:31ZengNature PortfolioScientific Reports2045-23222022-10-0112111410.1038/s41598-022-22252-7An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC)Arsham Mikaeili Namini0Motahareh Jahangir1Maryam Mohseni2Ali Asghar Kolahi3Hossein Hassanian-Moghaddam4Zeinab Mazloumi5Marzieh Motallebi6Mojgan Sheikhpour7Abolfazl Movafagh8Department of Animal Biology, Faculty of Biological Sciences, Kharazmi UniversityDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti UniversityDepartment of Social Medicine, School of Medicine, Shahid Beheshti University of Medical SciencesSocial Determinants of Health Research Center, Shahid Beheshti University of Medical SciencesSocial Determinants of Health Research Center, Shahid Beheshti University of Medical SciencesDepartment of Biology, Zanjan Branch, Islamic Azad UniversityDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Microbiology Research Center (MRC), Pasteur Institute of IranDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical SciencesAbstract Small cell lung cancer (SCLC) is a particularly lethal subtype of lung cancer. Metastatic lung tumours lead to most deaths from lung cancer. Predicting and preventing tumour metastasis is crucially essential for patient survivability. Hence, in the current study, we focused on a comprehensive analysis of lung cancer patients' differentially expressed genes (DEGs) on brain metastasis cell lines. DEGs are analysed through KEGG and GO databases for the most critical biological processes and pathways for enriched DEGs. Additionally, we performed protein–protein interaction (PPI), GeneMANIA, and Kaplan–Meier survival analyses on our DEGs. This article focused on mRNA and lncRNA DEGs for LC patients with brain metastasis and underlying molecular mechanisms. The expression data was gathered from the Gene Expression Omnibus database (GSE161968). We demonstrate that 30 distinct genes are up-expressed in brain metastatic SCLC patients, and 31 genes are down-expressed. All our analyses show that these genes are involved in metastatic SCLC. PPI analysis revealed two hub genes (CAT and APP). The results of this article present three lncRNAs, Including XLOC_l2_000941, LOC100507481, and XLOC_l2_007062, also notable mRNAs, have a close relation with brain metastasis in lung cancer and may have a role in the epithelial-mesenchymal transition (EMT) in tumour cells.https://doi.org/10.1038/s41598-022-22252-7 |
| spellingShingle | Arsham Mikaeili Namini Motahareh Jahangir Maryam Mohseni Ali Asghar Kolahi Hossein Hassanian-Moghaddam Zeinab Mazloumi Marzieh Motallebi Mojgan Sheikhpour Abolfazl Movafagh An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) Scientific Reports |
| title | An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) |
| title_full | An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) |
| title_fullStr | An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) |
| title_full_unstemmed | An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) |
| title_short | An in silico comparative transcriptome analysis identifying hub lncRNAs and mRNAs in brain metastatic small cell lung cancer (SCLC) |
| title_sort | in silico comparative transcriptome analysis identifying hub lncrnas and mrnas in brain metastatic small cell lung cancer sclc |
| url | https://doi.org/10.1038/s41598-022-22252-7 |
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