Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life
Ben-Zhen Chen,1,2,* Li-Hua Jiang,3,* Wenqin Zhou,4 Yu-Chao Shang,4 Fang Li,1 Bin Liu1 1Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2Department of Anesthesiology, Sichuan Provincial Women’s and Children’s Hospital, Chengd...
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Dove Medical Press
2022-10-01
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| Series: | Journal of Pain Research |
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| Online Access: | https://www.dovepress.com/repeated-sevoflurane-exposure-in-neonatal-rats-enhances-the-sensitivit-peer-reviewed-fulltext-article-JPR |
| _version_ | 1811239954650497024 |
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| author | Chen BZ Jiang LH Zhou W Shang YC Li F Liu B |
| author_facet | Chen BZ Jiang LH Zhou W Shang YC Li F Liu B |
| author_sort | Chen BZ |
| collection | DOAJ |
| description | Ben-Zhen Chen,1,2,* Li-Hua Jiang,3,* Wenqin Zhou,4 Yu-Chao Shang,4 Fang Li,1 Bin Liu1 1Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2Department of Anesthesiology, Sichuan Provincial Women’s and Children’s Hospital, Chengdu, People’s Republic of China; 3Department of Operating Room Nursing, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 4Department of Anesthesiology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bin Liu, Department of Anesthesiology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, Sichuan, 610041, People’s Republic of China, Tel +86-13408669172, Email liubinhxyy@163.comPurposeː: Sevoflurane exposure in the neonatal period of rodent animals was reported to be associated with neuroendocrine dysregulations later in life. We tested the hypothesis that repeated sevoflurane exposure in neonatal rats enhances the sensitivity to pain and acute traumatic stress response later in juvenile life and investigated whether the neonatal brain depolarizing γ-aminobutyric acid type A receptor (GABAAR) activity is involved in mediating these abnormalities.Methodsː: The postnatal 6 days (P6) Sprague-Dawley male rat pups pretreated with vehicle or the NKCC1 inhibitor, bumetanide, received sequential exposures to 2.1% sevoflurane exposure for 2 hours daily in 3 consecutive days.Resultsː: The results showed that repeated exposures to sevoflurane in neonatal rats significantly reduced the paw withdrawal thermal latency (PWTL) at P9, P45. Repeated exposures to sevoflurane in neonatal rats did not significantly affect the basal secretion of serum corticosterone at juvenile period P45, whereas the level of corticosterone for neonatal sevoflurane-exposed rats at P45 was significantly higher than the CON group after subject to conditioned fear traumatic stress (CFTS). The resulting NKCC1/KCC2 mRNA ratio was significantly increased immediately after the neonatal rats received the last sevoflurane exposure, which was alleviated by pretreated with the NKCC1 inhibitor bumetanide.Conclusionː: Repeated exposures to sevoflurane in neonatal rats enhanced the sensitivity to pain and acute traumatic stress response in juvenile life. The neonatal brain depolarizing GABAAR activity is involved in mediating these abnormalities.Keywords: sevoflurane, neonatal, exposure, paw withdrawal thermal latency, conditioned fear traumatic stress |
| first_indexed | 2024-04-12T13:10:51Z |
| format | Article |
| id | doaj.art-71bcc65598c94e1f9ad5456c4e5d1f15 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-04-12T13:10:51Z |
| publishDate | 2022-10-01 |
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| series | Journal of Pain Research |
| spelling | doaj.art-71bcc65598c94e1f9ad5456c4e5d1f152022-12-22T03:31:52ZengDove Medical PressJournal of Pain Research1178-70902022-10-01Volume 153171317878902Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile LifeChen BZJiang LHZhou WShang YCLi FLiu BBen-Zhen Chen,1,2,* Li-Hua Jiang,3,* Wenqin Zhou,4 Yu-Chao Shang,4 Fang Li,1 Bin Liu1 1Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2Department of Anesthesiology, Sichuan Provincial Women’s and Children’s Hospital, Chengdu, People’s Republic of China; 3Department of Operating Room Nursing, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 4Department of Anesthesiology, West China Second University Hospital, Sichuan University, Chengdu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bin Liu, Department of Anesthesiology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, Sichuan, 610041, People’s Republic of China, Tel +86-13408669172, Email liubinhxyy@163.comPurposeː: Sevoflurane exposure in the neonatal period of rodent animals was reported to be associated with neuroendocrine dysregulations later in life. We tested the hypothesis that repeated sevoflurane exposure in neonatal rats enhances the sensitivity to pain and acute traumatic stress response later in juvenile life and investigated whether the neonatal brain depolarizing γ-aminobutyric acid type A receptor (GABAAR) activity is involved in mediating these abnormalities.Methodsː: The postnatal 6 days (P6) Sprague-Dawley male rat pups pretreated with vehicle or the NKCC1 inhibitor, bumetanide, received sequential exposures to 2.1% sevoflurane exposure for 2 hours daily in 3 consecutive days.Resultsː: The results showed that repeated exposures to sevoflurane in neonatal rats significantly reduced the paw withdrawal thermal latency (PWTL) at P9, P45. Repeated exposures to sevoflurane in neonatal rats did not significantly affect the basal secretion of serum corticosterone at juvenile period P45, whereas the level of corticosterone for neonatal sevoflurane-exposed rats at P45 was significantly higher than the CON group after subject to conditioned fear traumatic stress (CFTS). The resulting NKCC1/KCC2 mRNA ratio was significantly increased immediately after the neonatal rats received the last sevoflurane exposure, which was alleviated by pretreated with the NKCC1 inhibitor bumetanide.Conclusionː: Repeated exposures to sevoflurane in neonatal rats enhanced the sensitivity to pain and acute traumatic stress response in juvenile life. The neonatal brain depolarizing GABAAR activity is involved in mediating these abnormalities.Keywords: sevoflurane, neonatal, exposure, paw withdrawal thermal latency, conditioned fear traumatic stresshttps://www.dovepress.com/repeated-sevoflurane-exposure-in-neonatal-rats-enhances-the-sensitivit-peer-reviewed-fulltext-article-JPRsevofluraneneonatalexposurepaw withdrawl thermal latencyconditioned fear traumatic stress |
| spellingShingle | Chen BZ Jiang LH Zhou W Shang YC Li F Liu B Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life Journal of Pain Research sevoflurane neonatal exposure paw withdrawl thermal latency conditioned fear traumatic stress |
| title | Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life |
| title_full | Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life |
| title_fullStr | Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life |
| title_full_unstemmed | Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life |
| title_short | Repeated Sevoflurane Exposure in Neonatal Rats Enhances the Sensitivity to Pain and Traumatic Stress Later in Juvenile Life |
| title_sort | repeated sevoflurane exposure in neonatal rats enhances the sensitivity to pain and traumatic stress later in juvenile life |
| topic | sevoflurane neonatal exposure paw withdrawl thermal latency conditioned fear traumatic stress |
| url | https://www.dovepress.com/repeated-sevoflurane-exposure-in-neonatal-rats-enhances-the-sensitivit-peer-reviewed-fulltext-article-JPR |
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