Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India
Introduction: Haemophilus influenzae is responsible for wide range of localized and invasive lower respiratory tract infections (LRTI) with the highest burden of disease in low and middle income countries. Aim: The aim of the present study was to characterize the H.influenzae isolates from susp...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/7892/18612_CE(Ra1)_F(GH)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1819024178563514368 |
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| author | Padmaja Ananth Shenoy Kiran Chawla Shashidhar Vishwanath Dipika Shaw |
| author_facet | Padmaja Ananth Shenoy Kiran Chawla Shashidhar Vishwanath Dipika Shaw |
| author_sort | Padmaja Ananth Shenoy |
| collection | DOAJ |
| description | Introduction: Haemophilus influenzae is responsible for wide
range of localized and invasive lower respiratory tract infections
(LRTI) with the highest burden of disease in low and middle
income countries.
Aim: The aim of the present study was to characterize the
H.influenzae isolates from suspected LRTI.
Materials and Methods: A prospective study was conducted
over a period of one and half years (December 2012 to May
2014) including patients with LRTI. H.influenzae was isolated from
lower respiratory specimens following standard procedures.
Complete characterization of the isolates was performed by
bio typing, capsular serotyping, molecular genotyping and
antibiotic susceptibility testing. The predisposing factors and
clinical presentation were studied in the infected patients.
Results: A total of 8995 samples were received during the study
period, out of which growth was significantly observed in 2848
(31.7%) samples. Among the various respiratory pathogens,
H.influenzae was isolated from 175 (6.14%) patients. Majority
(78.9%) of the patients presented with acute exacerbations
of chronic obstructive pulmonary disease. The isolates most
frequently were of Biotype II (35.42%). Only four of the 50
isolates subjected to capsular serotyping were typeable and
were of type b, e and f. All the 50 isolates tested were found
to be non-typeable by PCR for capsular genotyping. Maximum
resistance was found against ampicillin (9.71%).
Conclusion: H.influenzae was found to be a significant cause
of LRTI. Majority of the isolates were found to be non typeable
strains. Non typeable H. influenzae isolates should not be
neglected as they can colonize the respiratory tract in COPD
patients and can lead to biofilm formation and treatment
failure. |
| first_indexed | 2024-12-21T04:50:39Z |
| format | Article |
| id | doaj.art-71bdbd3bd7424195a60d977c21de65ff |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T04:50:39Z |
| publishDate | 2016-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-71bdbd3bd7424195a60d977c21de65ff2022-12-21T19:15:27ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-05-01105DC31DC3410.7860/JCDR/2016/18612.7892Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South IndiaPadmaja Ananth Shenoy0Kiran Chawla1Shashidhar Vishwanath2Dipika Shaw3Assistant Professor, Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.Professor and Head, Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.Associate Professor, Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.Post Graduate Student, Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.Introduction: Haemophilus influenzae is responsible for wide range of localized and invasive lower respiratory tract infections (LRTI) with the highest burden of disease in low and middle income countries. Aim: The aim of the present study was to characterize the H.influenzae isolates from suspected LRTI. Materials and Methods: A prospective study was conducted over a period of one and half years (December 2012 to May 2014) including patients with LRTI. H.influenzae was isolated from lower respiratory specimens following standard procedures. Complete characterization of the isolates was performed by bio typing, capsular serotyping, molecular genotyping and antibiotic susceptibility testing. The predisposing factors and clinical presentation were studied in the infected patients. Results: A total of 8995 samples were received during the study period, out of which growth was significantly observed in 2848 (31.7%) samples. Among the various respiratory pathogens, H.influenzae was isolated from 175 (6.14%) patients. Majority (78.9%) of the patients presented with acute exacerbations of chronic obstructive pulmonary disease. The isolates most frequently were of Biotype II (35.42%). Only four of the 50 isolates subjected to capsular serotyping were typeable and were of type b, e and f. All the 50 isolates tested were found to be non-typeable by PCR for capsular genotyping. Maximum resistance was found against ampicillin (9.71%). Conclusion: H.influenzae was found to be a significant cause of LRTI. Majority of the isolates were found to be non typeable strains. Non typeable H. influenzae isolates should not be neglected as they can colonize the respiratory tract in COPD patients and can lead to biofilm formation and treatment failure.https://jcdr.net/articles/PDF/7892/18612_CE(Ra1)_F(GH)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdfantibiotic susceptibility testingbiotypinggenotypingnon-typeable strains |
| spellingShingle | Padmaja Ananth Shenoy Kiran Chawla Shashidhar Vishwanath Dipika Shaw Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India Journal of Clinical and Diagnostic Research antibiotic susceptibility testing biotyping genotyping non-typeable strains |
| title | Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India |
| title_full | Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India |
| title_fullStr | Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India |
| title_full_unstemmed | Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India |
| title_short | Microbiological Characterization of Haemophilus influenzae Isolated from Patients with Lower Respiratory Tract Infections in a Tertiary Care Hospital, South India |
| title_sort | microbiological characterization of haemophilus influenzae isolated from patients with lower respiratory tract infections in a tertiary care hospital south india |
| topic | antibiotic susceptibility testing biotyping genotyping non-typeable strains |
| url | https://jcdr.net/articles/PDF/7892/18612_CE(Ra1)_F(GH)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
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