Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
Picobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises question...
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2021-07-01
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| author | Jose L. Huaman Carlo Pacioni Subir Sarker Mark Doyle David M. Forsyth Anthony Pople Jordan O. Hampton Teresa G. Carvalho Karla J. Helbig |
| author_facet | Jose L. Huaman Carlo Pacioni Subir Sarker Mark Doyle David M. Forsyth Anthony Pople Jordan O. Hampton Teresa G. Carvalho Karla J. Helbig |
| author_sort | Jose L. Huaman |
| collection | DOAJ |
| description | Picobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises questions concerning their tropism and pathogenicity. We report here PBV detection in wild deer and cattle from southeastern Australia. Through metagenomics, the presence of PBV genogroups I (GI) and II (GII) were detected in deer serum and plasma. Molecular epidemiology studies targeting the partial RNA-dependent RNA polymerase gene were performed in a wide range of specimens (serum, faeces, spleen, lung, nasal swabs, and trachea) collected from wild deer and cattle, with PCR amplification obtained in all specimen types except lung and spleen. Our results reveal the predominance of GI and concomitant detection of both genogroups in wild deer and cattle. In concordance with other studies, the detected GI sequences displayed high genetic diversity, however in contrast, GII sequences clustered into three distinct clades. Detection of both genogroups in the upper respiratory tract (trachea and nasal swab) of deer in the present study gives more evidence about the respiratory tract tropism of PBV. Although much remains unknown about the epidemiology and tropism of PBVs, our study suggests a wide distribution of these viruses in southeastern Australia. |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:18:38Z |
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| spelling | doaj.art-71bf94eea13b4e5c82792cf2359f566b2023-11-22T10:10:11ZengMDPI AGViruses1999-49152021-07-01138149210.3390/v13081492Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory TractJose L. Huaman0Carlo Pacioni1Subir Sarker2Mark Doyle3David M. Forsyth4Anthony Pople5Jordan O. Hampton6Teresa G. Carvalho7Karla J. Helbig8Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC 3086, AustraliaDepartment of Environment, Land, Water and Planning, Arthur Rylah Institute for Environmental Research, Heidelberg, VIC 3084, AustraliaDepartment of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC 3086, AustraliaSouth East Local Land Services, Bega, NSW 2550, AustraliaVertebrate Pest Research Unit, Department of Primary Industries, Orange Agricultural Institute, Orange, NSW 2800, AustraliaDepartment of Agriculture and Fisheries, Invasive Plants & Animals Research, Biosecurity Queensland, Ecosciences Precinct, Brisbane, QLD 4102, AustraliaSchool of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch, WA 6150, AustraliaDepartment of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC 3086, AustraliaDepartment of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC 3086, AustraliaPicobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises questions concerning their tropism and pathogenicity. We report here PBV detection in wild deer and cattle from southeastern Australia. Through metagenomics, the presence of PBV genogroups I (GI) and II (GII) were detected in deer serum and plasma. Molecular epidemiology studies targeting the partial RNA-dependent RNA polymerase gene were performed in a wide range of specimens (serum, faeces, spleen, lung, nasal swabs, and trachea) collected from wild deer and cattle, with PCR amplification obtained in all specimen types except lung and spleen. Our results reveal the predominance of GI and concomitant detection of both genogroups in wild deer and cattle. In concordance with other studies, the detected GI sequences displayed high genetic diversity, however in contrast, GII sequences clustered into three distinct clades. Detection of both genogroups in the upper respiratory tract (trachea and nasal swab) of deer in the present study gives more evidence about the respiratory tract tropism of PBV. Although much remains unknown about the epidemiology and tropism of PBVs, our study suggests a wide distribution of these viruses in southeastern Australia.https://www.mdpi.com/1999-4915/13/8/1492cattledeergenetic diversitymetagenomicspicobirnavirusRNA-dependent RNA polymerase |
| spellingShingle | Jose L. Huaman Carlo Pacioni Subir Sarker Mark Doyle David M. Forsyth Anthony Pople Jordan O. Hampton Teresa G. Carvalho Karla J. Helbig Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract Viruses cattle deer genetic diversity metagenomics picobirnavirus RNA-dependent RNA polymerase |
| title | Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract |
| title_full | Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract |
| title_fullStr | Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract |
| title_full_unstemmed | Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract |
| title_short | Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract |
| title_sort | molecular epidemiology and characterization of picobirnavirus in wild deer and cattle from australia evidence of genogroup i and ii in the upper respiratory tract |
| topic | cattle deer genetic diversity metagenomics picobirnavirus RNA-dependent RNA polymerase |
| url | https://www.mdpi.com/1999-4915/13/8/1492 |
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