Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients
Objective: Intensive care units are a hospital’s section where hospital infections and resistant microorganisms are most commonly seen. In this study it was aimed to determine the microorganisms which were isolated from various clinical specimens of the patients in intensive care units for a year an...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2017-04-01
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| Series: | Türk Yoğun Bakim Derneği Dergisi |
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| Online Access: | http://www.yogunbakimderg.com/article_14847/Distribution-Of-Clinical-Isolates-At-Species-Level-And-Their-Antibiotic-Susceptibilities-In-Intensive-Care-Units-Patients |
| _version_ | 1797907499765989376 |
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| author | Ayşe Barış Mehmet Emin Bulut Ahsen Öncül Banu Bayraktar |
| author_facet | Ayşe Barış Mehmet Emin Bulut Ahsen Öncül Banu Bayraktar |
| author_sort | Ayşe Barış |
| collection | DOAJ |
| description | Objective: Intensive care units are a hospital’s section where hospital infections and resistant microorganisms are most commonly seen. In this study it was aimed to determine the microorganisms which were isolated from various clinical specimens of the patients in intensive care units for a year and antibiotic susceptibility of the microorganisms.
Materials and Methods: MALDI TOF MS and BD Phoenix system were used for the identification of bacteria, antibiotic sensitivities were evaluated by Kirby-Bauer disk diffusion method and BD Phoenix system in accordance with the Clinical and Laboratory Standards Institute recommendations.
Results: In this study, a total of 1163 microorganisms were obtained; 575 (49.4%) gram-negative bacteria (GNB), 556 (47.8%) gram-positive bacteria (GPB) and 32 Candida spp. (2.7%). Strains were produced from blood (488), urine (233), respiratory tract (224), sterile body fluid (88), wounds (68) and catheter samples (62). The most frequently isolated GNBs were found to be Acinetobacter baumannii 131 (11.2%), Klebsiella pneumoniae 109 (9.3%), Escherichia coli 91 (7.8%) in order of frequency. Extended-spectrum beta-lactamase production was observed in 16 E. coli, 29 Klebsiella spp. Carbapenem resistance was identified in 132 Acinetobacter spp., 27 Pseudomonas spp., 14 K. pneumoniae, 1 E. coli. For Pseudomonas strains, ciprofloxacin and amikacin; for Acinetobacter strains, amikacin and colistin; for Escherichia and Klebsiella strains, amikacin and imipenem were determined as the most effective antibiotics. The most frequently isolated GPBs were 351 (30%) coagulase-negative staphylococci (CNS) (192 S. epidermidis), 111 (9.5%) Enterococcus spp. (67 Enterococcus faecalis), 55 Staphylococcus aureus, respectively. While Methicillin resistance was determined in 7 S. aureus and 191 CNS; vancomycin resistance was detected in 3 Enterococcus faecium strains. The most effective antibiotics against S. aureus and Enterococcus spp. strains were identified as linezolid, vancomycin, and teicoplanin.
Conclusion: It will be useful if every center arranges the treatment protocols according to their microorganism distribution and resistance profiles to fight against resistant microorganisms. |
| first_indexed | 2024-04-10T10:37:45Z |
| format | Article |
| id | doaj.art-71c002ea5fee427aa569edfdc7b1e44f |
| institution | Directory Open Access Journal |
| issn | 2146-6416 |
| language | English |
| last_indexed | 2024-04-10T10:37:45Z |
| publishDate | 2017-04-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türk Yoğun Bakim Derneği Dergisi |
| spelling | doaj.art-71c002ea5fee427aa569edfdc7b1e44f2023-02-15T16:20:45ZengGalenos YayineviTürk Yoğun Bakim Derneği Dergisi2146-64162017-04-01151212710.4274/tybdd.55707Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units PatientsAyşe Barış0Mehmet Emin Bulut1Ahsen Öncül2Banu Bayraktar3Şişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul, TürkiyeŞişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul, TürkiyeŞişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Enfeksiyon Hastalıkları Kliniği, İstanbul, TürkiyeŞişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul, TürkiyeObjective: Intensive care units are a hospital’s section where hospital infections and resistant microorganisms are most commonly seen. In this study it was aimed to determine the microorganisms which were isolated from various clinical specimens of the patients in intensive care units for a year and antibiotic susceptibility of the microorganisms. Materials and Methods: MALDI TOF MS and BD Phoenix system were used for the identification of bacteria, antibiotic sensitivities were evaluated by Kirby-Bauer disk diffusion method and BD Phoenix system in accordance with the Clinical and Laboratory Standards Institute recommendations. Results: In this study, a total of 1163 microorganisms were obtained; 575 (49.4%) gram-negative bacteria (GNB), 556 (47.8%) gram-positive bacteria (GPB) and 32 Candida spp. (2.7%). Strains were produced from blood (488), urine (233), respiratory tract (224), sterile body fluid (88), wounds (68) and catheter samples (62). The most frequently isolated GNBs were found to be Acinetobacter baumannii 131 (11.2%), Klebsiella pneumoniae 109 (9.3%), Escherichia coli 91 (7.8%) in order of frequency. Extended-spectrum beta-lactamase production was observed in 16 E. coli, 29 Klebsiella spp. Carbapenem resistance was identified in 132 Acinetobacter spp., 27 Pseudomonas spp., 14 K. pneumoniae, 1 E. coli. For Pseudomonas strains, ciprofloxacin and amikacin; for Acinetobacter strains, amikacin and colistin; for Escherichia and Klebsiella strains, amikacin and imipenem were determined as the most effective antibiotics. The most frequently isolated GPBs were 351 (30%) coagulase-negative staphylococci (CNS) (192 S. epidermidis), 111 (9.5%) Enterococcus spp. (67 Enterococcus faecalis), 55 Staphylococcus aureus, respectively. While Methicillin resistance was determined in 7 S. aureus and 191 CNS; vancomycin resistance was detected in 3 Enterococcus faecium strains. The most effective antibiotics against S. aureus and Enterococcus spp. strains were identified as linezolid, vancomycin, and teicoplanin. Conclusion: It will be useful if every center arranges the treatment protocols according to their microorganism distribution and resistance profiles to fight against resistant microorganisms.http://www.yogunbakimderg.com/article_14847/Distribution-Of-Clinical-Isolates-At-Species-Level-And-Their-Antibiotic-Susceptibilities-In-Intensive-Care-Units-PatientsIntensive care unitsinfectionantibiotic resistance |
| spellingShingle | Ayşe Barış Mehmet Emin Bulut Ahsen Öncül Banu Bayraktar Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients Türk Yoğun Bakim Derneği Dergisi Intensive care units infection antibiotic resistance |
| title | Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients |
| title_full | Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients |
| title_fullStr | Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients |
| title_full_unstemmed | Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients |
| title_short | Distribution of Clinical Isolates at Species Level and Their Antibiotic Susceptibilities in Intensive Care Units Patients |
| title_sort | distribution of clinical isolates at species level and their antibiotic susceptibilities in intensive care units patients |
| topic | Intensive care units infection antibiotic resistance |
| url | http://www.yogunbakimderg.com/article_14847/Distribution-Of-Clinical-Isolates-At-Species-Level-And-Their-Antibiotic-Susceptibilities-In-Intensive-Care-Units-Patients |
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