Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice
Abstract There is no blood biomarker diagnostic of arterial thrombosis. We investigated if arterial thrombosis per se was associated with alterations in complete blood count (CBC) and white blood cell (WBC) differential count in mice. Twelve-week-old C57Bl/6 mice were used for FeCl3-mediated carotid...
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| Format: | Article |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31122-9 |
| _version_ | 1827984295492321280 |
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| author | Hee Jeong Jang Dawid Schellingerhout Jiwon Kim Jinyong Chung Dong-Eog Kim |
| author_facet | Hee Jeong Jang Dawid Schellingerhout Jiwon Kim Jinyong Chung Dong-Eog Kim |
| author_sort | Hee Jeong Jang |
| collection | DOAJ |
| description | Abstract There is no blood biomarker diagnostic of arterial thrombosis. We investigated if arterial thrombosis per se was associated with alterations in complete blood count (CBC) and white blood cell (WBC) differential count in mice. Twelve-week-old C57Bl/6 mice were used for FeCl3-mediated carotid thrombosis (n = 72), sham-operation (n = 79), or non-operation (n = 26). Monocyte count (/µL) at 30-min after thrombosis (median 160 [interquartile range 140–280]) was ~ 1.3-fold higher than at 30-min after sham-operation (120 [77.5–170]), and twofold higher than in non-operated mice (80 [47.5–92.5]). At day-1 and -4 post-thrombosis, compared with 30-min, monocyte count decreased by about 6% and 28% to 150 [100–200] and 115 [100–127.5], which however were about 2.1-fold and 1.9-fold higher than in sham-operated mice (70 [50–100] and 60 [30–75], respectively). Lymphocyte counts (/µL) at 1- and 4-days after thrombosis (mean ± SD; 3513 ± 912 and 2590 ± 860) were ~ 38% and ~ 54% lower than those in the sham-operated mice (5630 ± 1602 and 5596 ± 1437, respectively), and ~ 39% and ~ 55% lower than those in non-operated mice (5791 ± 1344). Post-thrombosis monocyte-lymphocyte-ratio (MLR) was substantially higher at all three time-points (0.050 ± 0.02, 0.046 ± 0.025, and 0.050 ± 0.02) vs. sham (0.003 ± 0.021, 0.013 ± 0.004, and 0.010 ± 0.004). MLR was 0.013 ± 0.005 in non-operated mice. This is the first report on acute arterial thrombosis-related alterations in CBC and WBC differential parameters. |
| first_indexed | 2024-04-09T22:58:27Z |
| format | Article |
| id | doaj.art-71c0080ad3164ec1b6e06dff4ae47b4b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:58:27Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-71c0080ad3164ec1b6e06dff4ae47b4b2023-03-22T11:07:05ZengNature PortfolioScientific Reports2045-23222023-03-011311910.1038/s41598-023-31122-9Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in miceHee Jeong Jang0Dawid Schellingerhout1Jiwon Kim2Jinyong Chung3Dong-Eog Kim4Department of Medical Biotechnology, Dongguk UniversityDepartments of Diagnostic Radiology and Cancer Systems Imaging, University of Texas M. D. Anderson Cancer CenterMolecular Imaging and Neurovascular Research Laboratory, Department of Neurology, Dongguk University College of Medicine, Dongguk University Ilsan HospitalMedical Science Research Center, Dongguk University Medical CenterMolecular Imaging and Neurovascular Research Laboratory, Department of Neurology, Dongguk University College of Medicine, Dongguk University Ilsan HospitalAbstract There is no blood biomarker diagnostic of arterial thrombosis. We investigated if arterial thrombosis per se was associated with alterations in complete blood count (CBC) and white blood cell (WBC) differential count in mice. Twelve-week-old C57Bl/6 mice were used for FeCl3-mediated carotid thrombosis (n = 72), sham-operation (n = 79), or non-operation (n = 26). Monocyte count (/µL) at 30-min after thrombosis (median 160 [interquartile range 140–280]) was ~ 1.3-fold higher than at 30-min after sham-operation (120 [77.5–170]), and twofold higher than in non-operated mice (80 [47.5–92.5]). At day-1 and -4 post-thrombosis, compared with 30-min, monocyte count decreased by about 6% and 28% to 150 [100–200] and 115 [100–127.5], which however were about 2.1-fold and 1.9-fold higher than in sham-operated mice (70 [50–100] and 60 [30–75], respectively). Lymphocyte counts (/µL) at 1- and 4-days after thrombosis (mean ± SD; 3513 ± 912 and 2590 ± 860) were ~ 38% and ~ 54% lower than those in the sham-operated mice (5630 ± 1602 and 5596 ± 1437, respectively), and ~ 39% and ~ 55% lower than those in non-operated mice (5791 ± 1344). Post-thrombosis monocyte-lymphocyte-ratio (MLR) was substantially higher at all three time-points (0.050 ± 0.02, 0.046 ± 0.025, and 0.050 ± 0.02) vs. sham (0.003 ± 0.021, 0.013 ± 0.004, and 0.010 ± 0.004). MLR was 0.013 ± 0.005 in non-operated mice. This is the first report on acute arterial thrombosis-related alterations in CBC and WBC differential parameters.https://doi.org/10.1038/s41598-023-31122-9 |
| spellingShingle | Hee Jeong Jang Dawid Schellingerhout Jiwon Kim Jinyong Chung Dong-Eog Kim Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice Scientific Reports |
| title | Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| title_full | Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| title_fullStr | Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| title_full_unstemmed | Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| title_short | Towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| title_sort | towards a biomarker for acute arterial thrombosis using complete blood count and white blood cell differential parameters in mice |
| url | https://doi.org/10.1038/s41598-023-31122-9 |
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