Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation
Bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. We previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (BPH) fed on Bt rice line KMD2, although Bt insecticidal protein Cry1Ab could rarely c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01181/full |
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| author | Fang eWang Duo eNing Yang eChen Cong eDang Naishun eHan Yu'e eLiu Gong-Yin eYe |
| author_facet | Fang eWang Duo eNing Yang eChen Cong eDang Naishun eHan Yu'e eLiu Gong-Yin eYe |
| author_sort | Fang eWang |
| collection | DOAJ |
| description | Bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. We previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (BPH) fed on Bt rice line KMD2, although Bt insecticidal protein Cry1Ab could rarely concentrate in this non-target rice pest. In the present study, we performed microarray analysis in an effort to detect Bt-independent variation, which might render Bt rice more defensive and/or less nutritious to BPH. We detected 3,834 and 3,273 differentially expressed probe-sets in response to BPH infestation in non-Bt parent Xiushui 11 and Bt rice KMD2, respectively, only 439 of which showed significant differences in expression between rice lines. Our analysis revealed a shift from growth to defense responses in response to BPH infestation, which was also detected in many other studies of plants suffering biotic and abiotic stresses. Chlorophyll biosynthesis and basic metabolism pathways were inhibited in response to infestation. IAA and GA levels decreased as a result of the repression of biosynthesis-related genes or the induction of inactivation-related genes. In accordance with these observations, a number of IAA-, GA-, BR-signaling genes were downregulated in response to BPH. Thus, the growth of rice plants under BPH attack was reduced and defense related hormone signaling like JA, SA and ET were activated. In addition, growth-related hormone signaling pathways, such as GA, BR and auxin signaling pathways, as well as ABA, were also found to be involved in BPH-induced defense. On the other side, 51 probe-sets (represented 50 genes) that most likely contribute to the impact of Bt rice on BPH were identified, including three early nodulin genes, four lipid metabolic genes, 14 stress response genes, three TF genes and genes with other functions. Two transcription factor genes, bHLH and MYB, together with lipid transfer protein genes LTPL65 and early nodulin gene ENOD93, are the most likely candidates for improving herbivore resistance in plants. |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T00:25:30Z |
| publishDate | 2015-12-01 |
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| spelling | doaj.art-71c13ddfad0f49e886955d819f5cc8f92022-12-22T03:55:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-12-01610.3389/fpls.2015.01181169670Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestationFang eWang0Duo eNing1Yang eChen2Cong eDang3Naishun eHan4Yu'e eLiu5Gong-Yin eYe6Zhejiang UniversityZhejiang UniversityChina National Rice Research InstituteZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityBt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. We previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (BPH) fed on Bt rice line KMD2, although Bt insecticidal protein Cry1Ab could rarely concentrate in this non-target rice pest. In the present study, we performed microarray analysis in an effort to detect Bt-independent variation, which might render Bt rice more defensive and/or less nutritious to BPH. We detected 3,834 and 3,273 differentially expressed probe-sets in response to BPH infestation in non-Bt parent Xiushui 11 and Bt rice KMD2, respectively, only 439 of which showed significant differences in expression between rice lines. Our analysis revealed a shift from growth to defense responses in response to BPH infestation, which was also detected in many other studies of plants suffering biotic and abiotic stresses. Chlorophyll biosynthesis and basic metabolism pathways were inhibited in response to infestation. IAA and GA levels decreased as a result of the repression of biosynthesis-related genes or the induction of inactivation-related genes. In accordance with these observations, a number of IAA-, GA-, BR-signaling genes were downregulated in response to BPH. Thus, the growth of rice plants under BPH attack was reduced and defense related hormone signaling like JA, SA and ET were activated. In addition, growth-related hormone signaling pathways, such as GA, BR and auxin signaling pathways, as well as ABA, were also found to be involved in BPH-induced defense. On the other side, 51 probe-sets (represented 50 genes) that most likely contribute to the impact of Bt rice on BPH were identified, including three early nodulin genes, four lipid metabolic genes, 14 stress response genes, three TF genes and genes with other functions. Two transcription factor genes, bHLH and MYB, together with lipid transfer protein genes LTPL65 and early nodulin gene ENOD93, are the most likely candidates for improving herbivore resistance in plants.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01181/fullTranscription Factorshormonelipid transfer proteinBtBrown planthopperEarly nodulin |
| spellingShingle | Fang eWang Duo eNing Yang eChen Cong eDang Naishun eHan Yu'e eLiu Gong-Yin eYe Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation Frontiers in Plant Science Transcription Factors hormone lipid transfer protein Bt Brown planthopper Early nodulin |
| title | Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation |
| title_full | Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation |
| title_fullStr | Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation |
| title_full_unstemmed | Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation |
| title_short | Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation |
| title_sort | comparing gene expression profiles between bt and non bt rice in response to brown planthopper infestation |
| topic | Transcription Factors hormone lipid transfer protein Bt Brown planthopper Early nodulin |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01181/full |
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