Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan
Reports have documented antimicrobial usage in aquaculture, and the aquatic ecosystem can be considered a genetic storage site for antibiotic-resistant bacteria. This study assessed the prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria recovered from retail seafood in Hiroshi...
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MDPI AG
2023-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2935 |
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| author | Christian Xedzro Toshi Shimamoto Tadashi Shimamoto |
| author_facet | Christian Xedzro Toshi Shimamoto Tadashi Shimamoto |
| author_sort | Christian Xedzro |
| collection | DOAJ |
| description | Reports have documented antimicrobial usage in aquaculture, and the aquatic ecosystem can be considered a genetic storage site for antibiotic-resistant bacteria. This study assessed the prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria recovered from retail seafood in Hiroshima, Japan. A total of 412 bacteria were isolated and screened for the presence of β-lactamases, acquired carbapenemases, and mobile colistin-resistance (<i>mcr</i>) genes. Forty-five (10.9%) isolates were dominated by <i>Morganella</i> (28%), <i>Proteus</i> (22%), <i>Aeromonas</i> (14%), <i>Citrobacter</i> (8%), and <i>Escherichia</i> (8%) and carried AMR genes. The identified AMR genes included those encoded in integrons (19), <i>aac(6՛)-Ib</i> (11), <i>bla</i><sub>TEM-1</sub> (7), <i>bla</i><sub>CTX-M-like</sub> (12), <i>bla</i><sub>CTX-M-65</sub> (2), <i>bla</i><sub>SHV-12</sub> (1), <i>bla</i><sub>SHV-27</sub> (1), <i>bla</i><sub>OXA-10</sub> (1), <i>bla</i><sub>OXA-2</sub> (1), and <i>mcr</i> (2). The most common clinical resistances were against ampicillin, colistin, sulfamethoxazole/trimethoprim, tetracycline, and ciprofloxacin. Multidrug resistance (MDR) occurred in 27 (60%) AMR isolates, and multiple antibiotic resistance indices ranged from 0.2 to 0.8. A conjugation experiment showed that 10 of the 11 selected MDR strains harbored conjugable plasmids, although PCR-based replicon typing described seven strains as untypable. IncF replicon was identified in MDR extended-spectrum β-lactamase-producing <i>Escherichia coli</i> of the pathogenic B2 phylogroup. Our findings suggest that retail seafood harbors MDR bacteria of human interest that require strict resistance surveillance in the seafood production continuum. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:30:56Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-71c1fda7d56b499aa43e9cbab3d80bb32023-12-22T14:26:08ZengMDPI AGMicroorganisms2076-26072023-12-011112293510.3390/microorganisms11122935Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in JapanChristian Xedzro0Toshi Shimamoto1Tadashi Shimamoto2Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima 739-8528, JapanLaboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima 739-8528, JapanLaboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima 739-8528, JapanReports have documented antimicrobial usage in aquaculture, and the aquatic ecosystem can be considered a genetic storage site for antibiotic-resistant bacteria. This study assessed the prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria recovered from retail seafood in Hiroshima, Japan. A total of 412 bacteria were isolated and screened for the presence of β-lactamases, acquired carbapenemases, and mobile colistin-resistance (<i>mcr</i>) genes. Forty-five (10.9%) isolates were dominated by <i>Morganella</i> (28%), <i>Proteus</i> (22%), <i>Aeromonas</i> (14%), <i>Citrobacter</i> (8%), and <i>Escherichia</i> (8%) and carried AMR genes. The identified AMR genes included those encoded in integrons (19), <i>aac(6՛)-Ib</i> (11), <i>bla</i><sub>TEM-1</sub> (7), <i>bla</i><sub>CTX-M-like</sub> (12), <i>bla</i><sub>CTX-M-65</sub> (2), <i>bla</i><sub>SHV-12</sub> (1), <i>bla</i><sub>SHV-27</sub> (1), <i>bla</i><sub>OXA-10</sub> (1), <i>bla</i><sub>OXA-2</sub> (1), and <i>mcr</i> (2). The most common clinical resistances were against ampicillin, colistin, sulfamethoxazole/trimethoprim, tetracycline, and ciprofloxacin. Multidrug resistance (MDR) occurred in 27 (60%) AMR isolates, and multiple antibiotic resistance indices ranged from 0.2 to 0.8. A conjugation experiment showed that 10 of the 11 selected MDR strains harbored conjugable plasmids, although PCR-based replicon typing described seven strains as untypable. IncF replicon was identified in MDR extended-spectrum β-lactamase-producing <i>Escherichia coli</i> of the pathogenic B2 phylogroup. Our findings suggest that retail seafood harbors MDR bacteria of human interest that require strict resistance surveillance in the seafood production continuum.https://www.mdpi.com/2076-2607/11/12/2935antimicrobial resistanceretail seafoodplasmidsurveillanceJapan |
| spellingShingle | Christian Xedzro Toshi Shimamoto Tadashi Shimamoto Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan Microorganisms antimicrobial resistance retail seafood plasmid surveillance Japan |
| title | Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan |
| title_full | Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan |
| title_fullStr | Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan |
| title_full_unstemmed | Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan |
| title_short | Predominance of Multidrug-Resistant Gram-Negative Bacteria Isolated from Supermarket Retail Seafood in Japan |
| title_sort | predominance of multidrug resistant gram negative bacteria isolated from supermarket retail seafood in japan |
| topic | antimicrobial resistance retail seafood plasmid surveillance Japan |
| url | https://www.mdpi.com/2076-2607/11/12/2935 |
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