Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China
Soil fungi play a critical role in the carbon and nutrient cycling of forest ecosystems. Identifying the composition of soil fungi in response to the broadleaf restoration of <i>Pinus massoniana</i> plantation is essential for exploring the mechanistic linkages between tree species and e...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/13/12/2075 |
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| author | Linfang Wu Luhong Zhou Bingzhang Zou Sirong Wang Yong Zheng Zhiqun Huang Ji-Zheng He |
| author_facet | Linfang Wu Luhong Zhou Bingzhang Zou Sirong Wang Yong Zheng Zhiqun Huang Ji-Zheng He |
| author_sort | Linfang Wu |
| collection | DOAJ |
| description | Soil fungi play a critical role in the carbon and nutrient cycling of forest ecosystems. Identifying the composition of soil fungi in response to the broadleaf restoration of <i>Pinus massoniana</i> plantation is essential for exploring the mechanistic linkages between tree species and ecological processes, but remains unexplored. We compared the shifts in soil fungal diversity and guilds by high–throughput sequencing between two <i>P. massoniana</i> plantations at different stand ages, two modes of restoration with broadleaf trees, and a secondary forest in subtropical China. We found that soil fungal taxonomic and functional compositions significantly differed among forests. The highest Chao 1, Shannon, and phylogenetic diversity indices were consistently observed in the two <i>P. massoniana</i> monocultures, followed by the two modes of broadleaf mixing, and the secondary forests. Fungal communities transitioned from Ascomycota-dominated at <i>P. massoniana</i> plantations to Basidiomycota-dominated at other forests in the topsoil. Furthermore, saprotrophs and symbiotrophs were favoured in plantations and secondary forests, respectively. Soil pH exerted the most significant effect on the relative abundance of Ascomycota and Rozellomycota, as well as the saprotrophs. Moreover, the dominant phyla of Ascomycota, Mucoromycota, and Rozellomycota were negatively related to soil microbial biomass nitrogen, ammonium nitrogen, and total nitrogen contents; however, Mortierellomycota benefited from the elevated soil ammonium nitrogen content. On the other hand, soil nitrate nitrogen and available phosphorus contents strongly and negatively influenced the ectomycorrhizal fungi, while the other fungal guilds were mainly affected by soil pH. Our findings guide an evaluation of the consequences of forest restoration and contribute to an improved understanding of the mechanisms behind soil biogeochemical cycling in subtropical forest ecosystems. |
| first_indexed | 2024-03-09T16:38:14Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T16:38:14Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Forests |
| spelling | doaj.art-71c8826efd1440bcba27712fe461461f2023-11-24T14:54:58ZengMDPI AGForests1999-49072022-12-011312207510.3390/f13122075Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical ChinaLinfang Wu0Luhong Zhou1Bingzhang Zou2Sirong Wang3Yong Zheng4Zhiqun Huang5Ji-Zheng He6Key Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, ChinaKey Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, ChinaBaisha State-Owned Forest Farm of Fujian Province, Shanghang, Longyan 364205, ChinaBaisha State-Owned Forest Farm of Fujian Province, Shanghang, Longyan 364205, ChinaKey Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, ChinaKey Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, ChinaKey Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, ChinaSoil fungi play a critical role in the carbon and nutrient cycling of forest ecosystems. Identifying the composition of soil fungi in response to the broadleaf restoration of <i>Pinus massoniana</i> plantation is essential for exploring the mechanistic linkages between tree species and ecological processes, but remains unexplored. We compared the shifts in soil fungal diversity and guilds by high–throughput sequencing between two <i>P. massoniana</i> plantations at different stand ages, two modes of restoration with broadleaf trees, and a secondary forest in subtropical China. We found that soil fungal taxonomic and functional compositions significantly differed among forests. The highest Chao 1, Shannon, and phylogenetic diversity indices were consistently observed in the two <i>P. massoniana</i> monocultures, followed by the two modes of broadleaf mixing, and the secondary forests. Fungal communities transitioned from Ascomycota-dominated at <i>P. massoniana</i> plantations to Basidiomycota-dominated at other forests in the topsoil. Furthermore, saprotrophs and symbiotrophs were favoured in plantations and secondary forests, respectively. Soil pH exerted the most significant effect on the relative abundance of Ascomycota and Rozellomycota, as well as the saprotrophs. Moreover, the dominant phyla of Ascomycota, Mucoromycota, and Rozellomycota were negatively related to soil microbial biomass nitrogen, ammonium nitrogen, and total nitrogen contents; however, Mortierellomycota benefited from the elevated soil ammonium nitrogen content. On the other hand, soil nitrate nitrogen and available phosphorus contents strongly and negatively influenced the ectomycorrhizal fungi, while the other fungal guilds were mainly affected by soil pH. Our findings guide an evaluation of the consequences of forest restoration and contribute to an improved understanding of the mechanisms behind soil biogeochemical cycling in subtropical forest ecosystems.https://www.mdpi.com/1999-4907/13/12/2075biogeochemical processcarbon cyclingdiversityguildmycorrhizal fungitree species |
| spellingShingle | Linfang Wu Luhong Zhou Bingzhang Zou Sirong Wang Yong Zheng Zhiqun Huang Ji-Zheng He Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China Forests biogeochemical process carbon cycling diversity guild mycorrhizal fungi tree species |
| title | Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China |
| title_full | Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China |
| title_fullStr | Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China |
| title_full_unstemmed | Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China |
| title_short | Soil Fungal Diversity and Functionality Changes Associated with Multispecies Restoration of <i>Pinus massoniana</i> Plantation in Subtropical China |
| title_sort | soil fungal diversity and functionality changes associated with multispecies restoration of i pinus massoniana i plantation in subtropical china |
| topic | biogeochemical process carbon cycling diversity guild mycorrhizal fungi tree species |
| url | https://www.mdpi.com/1999-4907/13/12/2075 |
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