Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>)
The aquaporin (AQP) family, also called water channels or major intrinsic proteins, facilitate water transport. AQPs also transport low-molecular-weight solutes, including boric acid, glycerol, urea, and ammonia. Since plants are sessile, water homeostasis is crucial. Therefore, plants have develope...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/7/1511 |
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| author | Tamami Inden Atsushi Hoshino Shungo Otagaki Shogo Matsumoto Katsuhiro Shiratake |
| author_facet | Tamami Inden Atsushi Hoshino Shungo Otagaki Shogo Matsumoto Katsuhiro Shiratake |
| author_sort | Tamami Inden |
| collection | DOAJ |
| description | The aquaporin (AQP) family, also called water channels or major intrinsic proteins, facilitate water transport. AQPs also transport low-molecular-weight solutes, including boric acid, glycerol, urea, and ammonia. Since plants are sessile, water homeostasis is crucial. Therefore, plants have developed diverse AQP variants at higher expression levels than animals. For example, 35 and 33 AQPs have been identified in <i>Arabidopsis</i> and rice, respectively. In the present study, we identified AQPs in morning glory (<i>Ipomoea nil</i>), which has been widely used as a model plant in research on flowering and floral morphology. The importance of AQPs in the opening of morning glory flowers has been reported. In the morning glory genome, 44 AQPs were identified, and their characteristics were analyzed. A phylogenetic analysis revealed five AQP subfamilies in morning glory: plasma membrane-intrinsic proteins (PIPs), tonoplast-intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs), and X-intrinsic proteins (XIPs). Further, transport substrates of morning glory AQPs were estimated based on their homology to the known AQPs in other plant species and their corresponding amino acid motifs that possess permeability pores. It was expected that PIPs are likely to transport water, carbon dioxide, and hydrogen peroxide; TIPs are likely transport water, hydrogen peroxide, ammonia, urea, and boric acid; NIPs are likely transport water, boric acid, ammonia, glycerol, and formamide; and XIPs are likely to transport water, hydrogen peroxide, and glycerol. Overall, these results suggest that AQPs are involved in water and nutrient transport in Japanese morning glory. An in silico gene expression analysis suggested the importance of AQPs in flower opening, water or nutrient uptakes from the soil to roots, and photosynthesis in morning glory. Our findings provide fundamental information that enables further study into the importance of AQPs in morning glory, including their roles in flower opening and other physiological events. |
| first_indexed | 2024-03-11T05:27:49Z |
| format | Article |
| id | doaj.art-71cb016e0d6d46b1a281c15eb3b8a801 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T05:27:49Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-71cb016e0d6d46b1a281c15eb3b8a8012023-11-17T17:23:38ZengMDPI AGPlants2223-77472023-03-01127151110.3390/plants12071511Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>)Tamami Inden0Atsushi Hoshino1Shungo Otagaki2Shogo Matsumoto3Katsuhiro Shiratake4Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanNational Institute for Basic Biology, Okazaki 444-8585, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanThe aquaporin (AQP) family, also called water channels or major intrinsic proteins, facilitate water transport. AQPs also transport low-molecular-weight solutes, including boric acid, glycerol, urea, and ammonia. Since plants are sessile, water homeostasis is crucial. Therefore, plants have developed diverse AQP variants at higher expression levels than animals. For example, 35 and 33 AQPs have been identified in <i>Arabidopsis</i> and rice, respectively. In the present study, we identified AQPs in morning glory (<i>Ipomoea nil</i>), which has been widely used as a model plant in research on flowering and floral morphology. The importance of AQPs in the opening of morning glory flowers has been reported. In the morning glory genome, 44 AQPs were identified, and their characteristics were analyzed. A phylogenetic analysis revealed five AQP subfamilies in morning glory: plasma membrane-intrinsic proteins (PIPs), tonoplast-intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs), and X-intrinsic proteins (XIPs). Further, transport substrates of morning glory AQPs were estimated based on their homology to the known AQPs in other plant species and their corresponding amino acid motifs that possess permeability pores. It was expected that PIPs are likely to transport water, carbon dioxide, and hydrogen peroxide; TIPs are likely transport water, hydrogen peroxide, ammonia, urea, and boric acid; NIPs are likely transport water, boric acid, ammonia, glycerol, and formamide; and XIPs are likely to transport water, hydrogen peroxide, and glycerol. Overall, these results suggest that AQPs are involved in water and nutrient transport in Japanese morning glory. An in silico gene expression analysis suggested the importance of AQPs in flower opening, water or nutrient uptakes from the soil to roots, and photosynthesis in morning glory. Our findings provide fundamental information that enables further study into the importance of AQPs in morning glory, including their roles in flower opening and other physiological events.https://www.mdpi.com/2223-7747/12/7/1511genome-wide analysismorning gloryaquaporinAQP<i>Ipomoea nil</i> |
| spellingShingle | Tamami Inden Atsushi Hoshino Shungo Otagaki Shogo Matsumoto Katsuhiro Shiratake Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) Plants genome-wide analysis morning glory aquaporin AQP <i>Ipomoea nil</i> |
| title | Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) |
| title_full | Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) |
| title_fullStr | Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) |
| title_full_unstemmed | Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) |
| title_short | Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (<i>Ipomoea nil</i>) |
| title_sort | genome wide analysis of aquaporins in japanese morning glory i ipomoea nil i |
| topic | genome-wide analysis morning glory aquaporin AQP <i>Ipomoea nil</i> |
| url | https://www.mdpi.com/2223-7747/12/7/1511 |
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