NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i>
In addition to absorbing nitrogen from the soil, legumes have the ability to use atmospheric N<sub>2</sub> through symbiotic nitrogen fixation. Therefore, legumes have developed mechanisms regulating nodulation in response to the amount of nitrate in the soil; in the presence of high nit...
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MDPI AG
2024-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/322 |
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| author | Marie-Christine Morère-Le Paven Thibault Clochard Anis M. Limami |
| author_facet | Marie-Christine Morère-Le Paven Thibault Clochard Anis M. Limami |
| author_sort | Marie-Christine Morère-Le Paven |
| collection | DOAJ |
| description | In addition to absorbing nitrogen from the soil, legumes have the ability to use atmospheric N<sub>2</sub> through symbiotic nitrogen fixation. Therefore, legumes have developed mechanisms regulating nodulation in response to the amount of nitrate in the soil; in the presence of high nitrate concentrations, nodulation is inhibited, while low nitrate concentrations stimulate nodulation and nitrogen fixation. This allows the legumes to switch from soil nitrogen acquisition to symbiotic nitrogen fixation. Recently, particular interest has been given to the nitrate transporters, such as Nitrate Transporter1/Peptide transporter Family (NPF) and Nitrate Transporter 2 (NRT2), having a role in the functioning of nodules. Nitrate transporters of the two model plants, <i>Lotus japonicus</i> and <i>Medicago truncatula</i>, shown to have a positive and/or a negative role in nodule functioning depending on nitrate concentration, are presented in this article. In particular, the following transporters were thoroughly studied: (i) members of NPF transporters family, such as LjNPF8.6 and LjNPF3.1 in <i>L. japonicus</i> and MtNPF1.7 and MtNPF7.6 in <i>M. truncatula</i>, and (ii) members of NRT2 transporters family, such as LjNRT2.4 and LjNRT2.1 in <i>L. japonicus</i> and MtNRT2.1 in <i>M. truncatula</i>. Also, by exploiting available genomic and transcriptomic data in the literature, we have identified the complete PsNPF family in <i>Pisum sativum</i> (69 sequences previously described and 21 new that we have annotated) and putative nitrate transporters candidate for playing a role in nodule functioning in <i>P. sativum</i>. |
| first_indexed | 2024-03-08T09:47:48Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T09:47:48Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-71cd35de5ae24976bd8644c5becf93572024-01-29T14:12:30ZengMDPI AGPlants2223-77472024-01-0113232210.3390/plants13020322NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i>Marie-Christine Morère-Le Paven0Thibault Clochard1Anis M. Limami2Univ Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, 49000 Angers, FranceUniv Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, 49000 Angers, FranceUniv Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, 49000 Angers, FranceIn addition to absorbing nitrogen from the soil, legumes have the ability to use atmospheric N<sub>2</sub> through symbiotic nitrogen fixation. Therefore, legumes have developed mechanisms regulating nodulation in response to the amount of nitrate in the soil; in the presence of high nitrate concentrations, nodulation is inhibited, while low nitrate concentrations stimulate nodulation and nitrogen fixation. This allows the legumes to switch from soil nitrogen acquisition to symbiotic nitrogen fixation. Recently, particular interest has been given to the nitrate transporters, such as Nitrate Transporter1/Peptide transporter Family (NPF) and Nitrate Transporter 2 (NRT2), having a role in the functioning of nodules. Nitrate transporters of the two model plants, <i>Lotus japonicus</i> and <i>Medicago truncatula</i>, shown to have a positive and/or a negative role in nodule functioning depending on nitrate concentration, are presented in this article. In particular, the following transporters were thoroughly studied: (i) members of NPF transporters family, such as LjNPF8.6 and LjNPF3.1 in <i>L. japonicus</i> and MtNPF1.7 and MtNPF7.6 in <i>M. truncatula</i>, and (ii) members of NRT2 transporters family, such as LjNRT2.4 and LjNRT2.1 in <i>L. japonicus</i> and MtNRT2.1 in <i>M. truncatula</i>. Also, by exploiting available genomic and transcriptomic data in the literature, we have identified the complete PsNPF family in <i>Pisum sativum</i> (69 sequences previously described and 21 new that we have annotated) and putative nitrate transporters candidate for playing a role in nodule functioning in <i>P. sativum</i>.https://www.mdpi.com/2223-7747/13/2/322<i>Lotus japonicus</i><i>Medicago truncatula</i>nitrate transporternodulesNPFNRT2 |
| spellingShingle | Marie-Christine Morère-Le Paven Thibault Clochard Anis M. Limami NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> Plants <i>Lotus japonicus</i> <i>Medicago truncatula</i> nitrate transporter nodules NPF NRT2 |
| title | NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> |
| title_full | NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> |
| title_fullStr | NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> |
| title_full_unstemmed | NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> |
| title_short | NPF and NRT2 from <i>Pisum sativum</i> Potentially Involved in Nodule Functioning: Lessons from <i>Medicago truncatula</i> and <i>Lotus japonicus</i> |
| title_sort | npf and nrt2 from i pisum sativum i potentially involved in nodule functioning lessons from i medicago truncatula i and i lotus japonicus i |
| topic | <i>Lotus japonicus</i> <i>Medicago truncatula</i> nitrate transporter nodules NPF NRT2 |
| url | https://www.mdpi.com/2223-7747/13/2/322 |
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