Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu)
Corticeira-do-banhado is a native species, very common in southern Brazil with great potential in urban forestry. Efforts with the ge-nomic DNA isolation from leaves and exchange vascular E. crista-galli indi-cated contamination by polysaccharides or polyphenols. The Guapuruvu is native of Forest At...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Portuguese |
| Published: |
Instituto Federal de Educação, Ciência e Tecnologia Sul-Rio-Grandense
2015-12-01
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| Series: | Revista Thema |
| Subjects: | |
| Online Access: | http://revistathema.ifsul.edu.br/index.php/thema/article/view/283/143 |
| _version_ | 1818540392643035136 |
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| author | Luciano Moura de Mello Leonardo Severo da Costa Caetano Miguel Lemos Serrote Lia Rejane Reiniger |
| author_facet | Luciano Moura de Mello Leonardo Severo da Costa Caetano Miguel Lemos Serrote Lia Rejane Reiniger |
| author_sort | Luciano Moura de Mello |
| collection | DOAJ |
| description | Corticeira-do-banhado is a native species, very common in southern Brazil with great potential in urban forestry. Efforts with the ge-nomic DNA isolation from leaves and exchange vascular E. crista-galli indi-cated contamination by polysaccharides or polyphenols. The Guapuruvu is native of Forest Atlantic and the isolated leaves has used successfully in the assessment of genetic di-versity. The objective of this study was to develop a new and faster protocol for DNA isolation using seeds of these species. The proposed protocol showed bet-ter qualitative and quantitative results in re-lation to the sources previously evaluated for E. crista-galli and the average time of the technical implementa-tion was significantly lower. |
| first_indexed | 2024-12-11T21:54:45Z |
| format | Article |
| id | doaj.art-71cefeb225454a4a9f7e54f20a419e89 |
| institution | Directory Open Access Journal |
| issn | 2177-2894 |
| language | Portuguese |
| last_indexed | 2024-12-11T21:54:45Z |
| publishDate | 2015-12-01 |
| publisher | Instituto Federal de Educação, Ciência e Tecnologia Sul-Rio-Grandense |
| record_format | Article |
| series | Revista Thema |
| spelling | doaj.art-71cefeb225454a4a9f7e54f20a419e892022-12-22T00:49:21ZporInstituto Federal de Educação, Ciência e Tecnologia Sul-Rio-GrandenseRevista Thema2177-28942015-12-011201496010.15536/thema.12.2015.49-60.283Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu)Luciano Moura de MelloLeonardo Severo da CostaCaetano Miguel Lemos SerroteLia Rejane ReinigerCorticeira-do-banhado is a native species, very common in southern Brazil with great potential in urban forestry. Efforts with the ge-nomic DNA isolation from leaves and exchange vascular E. crista-galli indi-cated contamination by polysaccharides or polyphenols. The Guapuruvu is native of Forest Atlantic and the isolated leaves has used successfully in the assessment of genetic di-versity. The objective of this study was to develop a new and faster protocol for DNA isolation using seeds of these species. The proposed protocol showed bet-ter qualitative and quantitative results in re-lation to the sources previously evaluated for E. crista-galli and the average time of the technical implementa-tion was significantly lower.http://revistathema.ifsul.edu.br/index.php/thema/article/view/283/143genomic DNAseedembryo |
| spellingShingle | Luciano Moura de Mello Leonardo Severo da Costa Caetano Miguel Lemos Serrote Lia Rejane Reiniger Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) Revista Thema genomic DNA seed embryo |
| title | Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) |
| title_full | Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) |
| title_fullStr | Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) |
| title_full_unstemmed | Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) |
| title_short | Isolamento de DNA genômico utilizando sementes de Erythrina crista-galli L. (Corticeira-do-banhado) e Schizolobium parahyba (Vell.) Blake (Guapuruvu) |
| title_sort | isolamento de dna genomico utilizando sementes de erythrina crista galli l corticeira do banhado e schizolobium parahyba vell blake guapuruvu |
| topic | genomic DNA seed embryo |
| url | http://revistathema.ifsul.edu.br/index.php/thema/article/view/283/143 |
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