The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure
Bacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage fila...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/6/1163 |
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| author | Maximilian Haase Lutz Tessmer Lilian Köhnlechner Andreas Kuhn |
| author_facet | Maximilian Haase Lutz Tessmer Lilian Köhnlechner Andreas Kuhn |
| author_sort | Maximilian Haase |
| collection | DOAJ |
| description | Bacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage filament, which assembles multiple copies of the membrane-inserted major coat protein G8p around the extruding single-stranded circular DNA. The phage filament then passes through the G4p secretin that is localized in the outer membrane. Presumably, the inner membrane G1p/G11p and the outer G4p form a common complex. To unravel the structural details of the M13 assembly machine, we purified G1p from infected <i>E. coli</i> cells. The protein was overproduced together with G11p and solubilized from the membrane as a multimeric complex with a size of about 320 kDa. The complex revealed a pore-like structure with an outer diameter of about 12 nm, matching the dimensions of the outer membrane G4p secretin. The function of the M13 assembly machine for phage generation and secretion is discussed. |
| first_indexed | 2024-03-09T22:15:20Z |
| format | Article |
| id | doaj.art-71cf6dc011fc49df9ef20c8b9d8d1b79 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:15:20Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-71cf6dc011fc49df9ef20c8b9d8d1b792023-11-23T19:24:39ZengMDPI AGViruses1999-49152022-05-01146116310.3390/v14061163The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring StructureMaximilian Haase0Lutz Tessmer1Lilian Köhnlechner2Andreas Kuhn3Institute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Biology, University of Hohenheim, 70599 Stuttgart, GermanyBacteriophage M13 assembles its progeny particles in the inner membrane of the host. The major component of the assembly machine is G1p and together with G11p it generates an oligomeric structure with a pore-like inner cavity and an ATP hydrolysing domain. This allows the formation of the phage filament, which assembles multiple copies of the membrane-inserted major coat protein G8p around the extruding single-stranded circular DNA. The phage filament then passes through the G4p secretin that is localized in the outer membrane. Presumably, the inner membrane G1p/G11p and the outer G4p form a common complex. To unravel the structural details of the M13 assembly machine, we purified G1p from infected <i>E. coli</i> cells. The protein was overproduced together with G11p and solubilized from the membrane as a multimeric complex with a size of about 320 kDa. The complex revealed a pore-like structure with an outer diameter of about 12 nm, matching the dimensions of the outer membrane G4p secretin. The function of the M13 assembly machine for phage generation and secretion is discussed.https://www.mdpi.com/1999-4915/14/6/1163bacteriophage M13membrane proteinaffinity chromatographycircular dichroismphage assembly machine |
| spellingShingle | Maximilian Haase Lutz Tessmer Lilian Köhnlechner Andreas Kuhn The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure Viruses bacteriophage M13 membrane protein affinity chromatography circular dichroism phage assembly machine |
| title | The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure |
| title_full | The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure |
| title_fullStr | The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure |
| title_full_unstemmed | The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure |
| title_short | The M13 Phage Assembly Machine Has a Membrane-Spanning Oligomeric Ring Structure |
| title_sort | m13 phage assembly machine has a membrane spanning oligomeric ring structure |
| topic | bacteriophage M13 membrane protein affinity chromatography circular dichroism phage assembly machine |
| url | https://www.mdpi.com/1999-4915/14/6/1163 |
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