Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study
Method: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. Results: In the total part...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Family Medicine and Primary Care |
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| Online Access: | http://www.jfmpc.com/article.asp?issn=2249-4863;year=2019;volume=8;issue=9;spage=2937;epage=2941;aulast=Kumar |
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| author | Puneet Kumar Aarti Sharma Siddharth Das Ragini Srivastava Nikhil Gupta |
| author_facet | Puneet Kumar Aarti Sharma Siddharth Das Ragini Srivastava Nikhil Gupta |
| author_sort | Puneet Kumar |
| collection | DOAJ |
| description | Method: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. Results: In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors' samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19–1.98, P = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with P value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29–2.27, P = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of ANKH-4 G-to-A gene polymorphism in studied subjects. Conclusion: This study did not show any significant G to A mutation in the studied subjects. |
| first_indexed | 2024-12-11T20:00:07Z |
| format | Article |
| id | doaj.art-71d115dd44ea428ebd4d99a45a8104f4 |
| institution | Directory Open Access Journal |
| issn | 2249-4863 |
| language | English |
| last_indexed | 2024-12-11T20:00:07Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Family Medicine and Primary Care |
| spelling | doaj.art-71d115dd44ea428ebd4d99a45a8104f42022-12-22T00:52:32ZengWolters Kluwer Medknow PublicationsJournal of Family Medicine and Primary Care2249-48632019-01-01892937294110.4103/jfmpc.jfmpc_471_19Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional studyPuneet KumarAarti SharmaSiddharth DasRagini SrivastavaNikhil GuptaMethod: A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5′-untranslated region of ankylosis human (ANKH) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India. Results: In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors' samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19–1.98, P = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with P value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29–2.27, P = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of ANKH-4 G-to-A gene polymorphism in studied subjects. Conclusion: This study did not show any significant G to A mutation in the studied subjects.http://www.jfmpc.com/article.asp?issn=2249-4863;year=2019;volume=8;issue=9;spage=2937;epage=2941;aulast=KumarAnkylosis humanchondrocalcinosisgenotypeKhatriOAosteoarthritis |
| spellingShingle | Puneet Kumar Aarti Sharma Siddharth Das Ragini Srivastava Nikhil Gupta Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study Journal of Family Medicine and Primary Care Ankylosis human chondrocalcinosis genotype Khatri OA osteoarthritis |
| title | Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study |
| title_full | Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study |
| title_fullStr | Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study |
| title_full_unstemmed | Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study |
| title_short | Association of 4-basepair G-to-A transition in the 5′-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis: A cross sectional study |
| title_sort | association of 4 basepair g to a transition in the 5 untranslated region of ankh gene with selected patients of primary knee osteoarthritis a cross sectional study |
| topic | Ankylosis human chondrocalcinosis genotype Khatri OA osteoarthritis |
| url | http://www.jfmpc.com/article.asp?issn=2249-4863;year=2019;volume=8;issue=9;spage=2937;epage=2941;aulast=Kumar |
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