Acute fat loss does not affect bone mass
Abstract Obesity has previously been thought to protect bone since high body weight and body mass index are associated with high bone mass. However, some more recent studies suggest that increased adiposity negatively impacts bone mass. Here, we aimed to test whether acute loss of adipose tissue, vi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-93450-y |
| _version_ | 1818433020064956416 |
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| author | Marie K. Lagerquist Karin L. Gustafsson Petra Henning Helen Farman Jianyao Wu Klara Sjögren Antti Koskela Juha Tuukkanen Claes Ohlsson Ingrid Wernstedt Asterholm Louise Grahnemo |
| author_facet | Marie K. Lagerquist Karin L. Gustafsson Petra Henning Helen Farman Jianyao Wu Klara Sjögren Antti Koskela Juha Tuukkanen Claes Ohlsson Ingrid Wernstedt Asterholm Louise Grahnemo |
| author_sort | Marie K. Lagerquist |
| collection | DOAJ |
| description | Abstract Obesity has previously been thought to protect bone since high body weight and body mass index are associated with high bone mass. However, some more recent studies suggest that increased adiposity negatively impacts bone mass. Here, we aimed to test whether acute loss of adipose tissue, via adipocyte apoptosis, alters bone mass in age-related obese mice. Adipocyte apoptosis was induced in obese male FAT-ATTAC mice through AP20187 dimerizer-mediated activation of caspase 8 selectively in adipocytes. In a short-term experiment, dimerizer was administered to 5.5 month-old mice that were terminated 2 weeks later. At termination, the total fat mass weighed 58% less in dimerizer-treated mice compared with vehicle-treated controls, but bone mass did not differ. To allow for the detection of long-term effects, we used 9-month-old mice that were terminated six weeks after dimerizer administration. In this experiment, the total fat mass weighed less (− 68%) in the dimerizer-treated mice than in the controls, yet neither bone mass nor biomechanical properties differed between groups. Our findings show that adipose tissue loss, despite the reduced mechanical loading, does not affect bone in age-related obese mice. Future studies are needed to test whether adipose tissue loss is beneficial during more severe obesity. |
| first_indexed | 2024-12-14T16:14:27Z |
| format | Article |
| id | doaj.art-71d40cd8466842cca13db51afcfedbdf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:14:27Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-71d40cd8466842cca13db51afcfedbdf2022-12-21T22:54:56ZengNature PortfolioScientific Reports2045-23222021-07-0111111310.1038/s41598-021-93450-yAcute fat loss does not affect bone massMarie K. Lagerquist0Karin L. Gustafsson1Petra Henning2Helen Farman3Jianyao Wu4Klara Sjögren5Antti Koskela6Juha Tuukkanen7Claes Ohlsson8Ingrid Wernstedt Asterholm9Louise Grahnemo10Centre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgDepartment of Anatomy and Cell Biology, Cancer and Translational Medicine Research Unit, University of OuluDepartment of Anatomy and Cell Biology, Cancer and Translational Medicine Research Unit, University of OuluCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgUnit of Metabolic Physiology, Department of Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of GothenburgCentre for Bone and Arthritis Research, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, The Sahlgrenska Academy, University of GothenburgAbstract Obesity has previously been thought to protect bone since high body weight and body mass index are associated with high bone mass. However, some more recent studies suggest that increased adiposity negatively impacts bone mass. Here, we aimed to test whether acute loss of adipose tissue, via adipocyte apoptosis, alters bone mass in age-related obese mice. Adipocyte apoptosis was induced in obese male FAT-ATTAC mice through AP20187 dimerizer-mediated activation of caspase 8 selectively in adipocytes. In a short-term experiment, dimerizer was administered to 5.5 month-old mice that were terminated 2 weeks later. At termination, the total fat mass weighed 58% less in dimerizer-treated mice compared with vehicle-treated controls, but bone mass did not differ. To allow for the detection of long-term effects, we used 9-month-old mice that were terminated six weeks after dimerizer administration. In this experiment, the total fat mass weighed less (− 68%) in the dimerizer-treated mice than in the controls, yet neither bone mass nor biomechanical properties differed between groups. Our findings show that adipose tissue loss, despite the reduced mechanical loading, does not affect bone in age-related obese mice. Future studies are needed to test whether adipose tissue loss is beneficial during more severe obesity.https://doi.org/10.1038/s41598-021-93450-y |
| spellingShingle | Marie K. Lagerquist Karin L. Gustafsson Petra Henning Helen Farman Jianyao Wu Klara Sjögren Antti Koskela Juha Tuukkanen Claes Ohlsson Ingrid Wernstedt Asterholm Louise Grahnemo Acute fat loss does not affect bone mass Scientific Reports |
| title | Acute fat loss does not affect bone mass |
| title_full | Acute fat loss does not affect bone mass |
| title_fullStr | Acute fat loss does not affect bone mass |
| title_full_unstemmed | Acute fat loss does not affect bone mass |
| title_short | Acute fat loss does not affect bone mass |
| title_sort | acute fat loss does not affect bone mass |
| url | https://doi.org/10.1038/s41598-021-93450-y |
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