Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae)
Premise of the study: A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus <i>Ostryopsis</i> (Betulaceae). Methods and Results: A total of 38 high-quality simple sequence repeat (S...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-02-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300062 |
| _version_ | 1819098284957892608 |
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| author | Bing-Bing Liu Bin Tian Hui Ma Zhi-Qiang Lu Qiang Qiu Kang-Shan Mao Jian-Quan Liu |
| author_facet | Bing-Bing Liu Bin Tian Hui Ma Zhi-Qiang Lu Qiang Qiu Kang-Shan Mao Jian-Quan Liu |
| author_sort | Bing-Bing Liu |
| collection | DOAJ |
| description | Premise of the study: A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus <i>Ostryopsis</i> (Betulaceae).
Methods and Results: A total of 38 high-quality simple sequence repeat (SSR) primers were identified, of which 15 could be successfully amplified. Subsequently, we selected 80 individuals to represent the three species of the genus to evaluate the efficacy of these markers for examining genetic diversity of each species in the future. We found that the number of alleles per locus ranged from one to nine, with an average of 3.8. The expected heterozygosity and observed heterozygosity per locus varied from 0 to 0.829 and from 0 to 1, respectively, with their respective mean values as 0.483 and 0.416.
Conclusions: These EST-SSR markers will be useful for evaluating the range-wide genetic diversity of each species and examining genetic divergence and gene flow between the three species. |
| first_indexed | 2024-12-22T00:28:33Z |
| format | Article |
| id | doaj.art-71d79546b26242c5a6cdafffaa94af8f |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-22T00:28:33Z |
| publishDate | 2014-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-71d79546b26242c5a6cdafffaa94af8f2022-12-21T18:44:59ZengWileyApplications in Plant Sciences2168-04502014-02-0122130006210.3732/apps.1300062Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae)Bing-Bing Liu0Bin Tian1Hui Ma2Zhi-Qiang Lu3Qiang Qiu4Kang-Shan Mao5Jian-Quan Liu6State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China; Key Laboratory for Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, People's Republic of ChinaKey Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of ChinaState Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of ChinaState Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of ChinaState Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of ChinaKey Laboratory for Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, People's Republic of ChinaState Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of ChinaPremise of the study: A set of expressed sequence tag (EST) microsatellite markers were developed and characterized using next-generation sequencing technology for the Chinese genus <i>Ostryopsis</i> (Betulaceae). Methods and Results: A total of 38 high-quality simple sequence repeat (SSR) primers were identified, of which 15 could be successfully amplified. Subsequently, we selected 80 individuals to represent the three species of the genus to evaluate the efficacy of these markers for examining genetic diversity of each species in the future. We found that the number of alleles per locus ranged from one to nine, with an average of 3.8. The expected heterozygosity and observed heterozygosity per locus varied from 0 to 0.829 and from 0 to 1, respectively, with their respective mean values as 0.483 and 0.416. Conclusions: These EST-SSR markers will be useful for evaluating the range-wide genetic diversity of each species and examining genetic divergence and gene flow between the three species.http://www.bioone.org/doi/full/10.3732/apps.1300062BetulaceaeEST-SSR markernext-generation sequencing technologyortholog genesOstryopsis |
| spellingShingle | Bing-Bing Liu Bin Tian Hui Ma Zhi-Qiang Lu Qiang Qiu Kang-Shan Mao Jian-Quan Liu Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) Applications in Plant Sciences Betulaceae EST-SSR marker next-generation sequencing technology ortholog genes Ostryopsis |
| title | Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) |
| title_full | Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) |
| title_fullStr | Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) |
| title_full_unstemmed | Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) |
| title_short | Development and Characterization of EST-SSR Markers in <i>Ostryopsis</i> (Betulaceae) |
| title_sort | development and characterization of est ssr markers in i ostryopsis i betulaceae |
| topic | Betulaceae EST-SSR marker next-generation sequencing technology ortholog genes Ostryopsis |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300062 |
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