Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52
C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two bioma...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal do Rio Grande
2020-06-01
|
| Series: | Vetor |
| Subjects: | |
| Online Access: | https://ojs2.furg.br/index.php?journal=vetor&page=article&op=view&path[]=7055 |
| _version_ | 1827983790223392768 |
|---|---|
| author | Luisa Sala Caroline Costa Moraes Susana Juliano Kalil |
| author_facet | Luisa Sala Caroline Costa Moraes Susana Juliano Kalil |
| author_sort | Luisa Sala |
| collection | DOAJ |
| description | C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass. |
| first_indexed | 2024-04-09T22:50:27Z |
| format | Article |
| id | doaj.art-71d8a171fe6e4fa1ab532ecccc055935 |
| institution | Directory Open Access Journal |
| issn | 0102-7352 2358-3452 |
| language | English |
| last_indexed | 2024-04-09T22:50:27Z |
| publishDate | 2020-06-01 |
| publisher | Universidade Federal do Rio Grande |
| record_format | Article |
| series | Vetor |
| spelling | doaj.art-71d8a171fe6e4fa1ab532ecccc0559352023-03-22T11:39:04ZengUniversidade Federal do Rio GrandeVetor0102-73522358-34522020-06-01271Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52Luisa Sala0Caroline Costa Moraes1Susana Juliano Kalil2Universidade Federal do Rio GrandeUniversidade Federal do PampaUniversidade Federal do Rio GrandeC-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass.https://ojs2.furg.br/index.php?journal=vetor&page=article&op=view&path[]=7055C-phycocyaninAllophycocyaninMicroalgaeUltrasound-assisted extractionHydratase. |
| spellingShingle | Luisa Sala Caroline Costa Moraes Susana Juliano Kalil Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 Vetor C-phycocyanin Allophycocyanin Microalgae Ultrasound-assisted extraction Hydratase. |
| title | Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 |
| title_full | Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 |
| title_fullStr | Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 |
| title_full_unstemmed | Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 |
| title_short | Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52 |
| title_sort | simultaneous extraction of phycobiliproteins and carbonic anhydrase from spirulina platensis leb 52 |
| topic | C-phycocyanin Allophycocyanin Microalgae Ultrasound-assisted extraction Hydratase. |
| url | https://ojs2.furg.br/index.php?journal=vetor&page=article&op=view&path[]=7055 |
| work_keys_str_mv | AT luisasala simultaneousextractionofphycobiliproteinsandcarbonicanhydrasefromspirulinaplatensisleb52 AT carolinecostamoraes simultaneousextractionofphycobiliproteinsandcarbonicanhydrasefromspirulinaplatensisleb52 AT susanajulianokalil simultaneousextractionofphycobiliproteinsandcarbonicanhydrasefromspirulinaplatensisleb52 |