Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)

Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen differ...

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Main Authors: Esra Bulunuz Palaz, Remzi Ugur
Format: Article
Language:English
Published: University of Life Sciences in Lublin - Publishing House 2022-08-01
Series:Acta Scientiarum Polonorum: Hortorum Cultus
Subjects:
Online Access:https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2189
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author Esra Bulunuz Palaz
Remzi Ugur
author_facet Esra Bulunuz Palaz
Remzi Ugur
author_sort Esra Bulunuz Palaz
collection DOAJ
description Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen different concentration of 6-benzylaminopurin, thidiazuron (TDZ), kinetin (KN) GA3 and naphthalene acetic acid (NAA) as plant growth regulators were used. In contrast to expectation, combinations of (TDZ 1.0 mg L–1 + GA3 0.25 mg L–1) and (TDZ 2.0 mg L–1 + GA3 0.25 mg L–1) were found to give positive results in shoot proliferation and plant formation at the end of the study. In the rooting study (NAA 0.5 mg L–1 + KN 0.1 mg L–1) and (NAA 0.5 mg L–1 + KN 0.2 mg L–1) treatment showed positive results.
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spelling doaj.art-71d91e3cfeaa4b2e86547478fa00e1b12023-12-12T09:40:03ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052022-08-0121410.24326/asphc.2022.4.1Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)Esra Bulunuz Palaz0Remzi Ugur1East Mediterranean Transitional Zone Agricultural Research of Institute, Kahramanmaras, TurkeyEast Mediterranean Transitional Zone Agricultural Research of Institute, Kahramanmaras, Turkey Murashige and Skoog (MS) medium supplemented with plant growth regulators composed of different concentrations were used in vitro rapid and mass multiplication of nodal explants obtained from shoots of black mulberry (Morus nigra L.) seedlings grown in a fully controlled greenhouse. Eighteen different concentration of 6-benzylaminopurin, thidiazuron (TDZ), kinetin (KN) GA3 and naphthalene acetic acid (NAA) as plant growth regulators were used. In contrast to expectation, combinations of (TDZ 1.0 mg L–1 + GA3 0.25 mg L–1) and (TDZ 2.0 mg L–1 + GA3 0.25 mg L–1) were found to give positive results in shoot proliferation and plant formation at the end of the study. In the rooting study (NAA 0.5 mg L–1 + KN 0.1 mg L–1) and (NAA 0.5 mg L–1 + KN 0.2 mg L–1) treatment showed positive results. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2189in vitroMS mediummicropropagationMorus nigra L.
spellingShingle Esra Bulunuz Palaz
Remzi Ugur
Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
Acta Scientiarum Polonorum: Hortorum Cultus
in vitro
MS medium
micropropagation
Morus nigra L.
title Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
title_full Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
title_fullStr Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
title_full_unstemmed Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
title_short Establishing an effective protocol for micropropagation of mullberry (Morus nigra L.)
title_sort establishing an effective protocol for micropropagation of mullberry morus nigra l
topic in vitro
MS medium
micropropagation
Morus nigra L.
url https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2189
work_keys_str_mv AT esrabulunuzpalaz establishinganeffectiveprotocolformicropropagationofmullberrymorusnigral
AT remziugur establishinganeffectiveprotocolformicropropagationofmullberrymorusnigral