Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus
It has been shown by bioinformatic methods that regions of the Bombyx mori viral nuclear polyhedrosis genome encoded two small RNA – snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleotide sequen...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Shirina_2_14.pdf |
| _version_ | 1827775481235111936 |
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| author | T. V. Shirina G. V. Gerashchenko M. T. Bobrovskaja V. I. Kashuba |
| author_facet | T. V. Shirina G. V. Gerashchenko M. T. Bobrovskaja V. I. Kashuba |
| author_sort | T. V. Shirina |
| collection | DOAJ |
| description | It has been shown by bioinformatic methods that regions of the Bombyx mori viral nuclear polyhedrosis genome encoded two small RNA – snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleotide sequence of small non-coding RNAs, predicted by bioinformatic methods in B. mori polyhedra. The following methods have been used: polymerase chain reaction, agarose gel electrophoresis, the cloning of PCR products, sequencing.
There were first determined nucleotide sequences of snc RNA-1 and snc RNA-2 of polyhedrin mRNA complementary regions which are included in B. mori polyhedra. These RNAs have 100% identity with bioinformatic predicted sequences. These results confirmed our bioinformatic approach to the search for small RNAs encoded in B. mori nuclear polyhedrosis virus genome. |
| first_indexed | 2024-03-11T13:50:13Z |
| format | Article |
| id | doaj.art-71da6b3e23394773a4a57d67d2a426b7 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:50:13Z |
| publishDate | 2014-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-71da6b3e23394773a4a57d67d2a426b72023-11-02T09:08:07ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032014-04-0186210110610.15407/ubj86.02.101Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virusT. V. Shirina0G. V. Gerashchenko1M. T. Bobrovskaja2V. I. Kashuba3Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivIt has been shown by bioinformatic methods that regions of the Bombyx mori viral nuclear polyhedrosis genome encoded two small RNA – snc RNA-1 and snc RNA-2, which could perform a structural function in polyhedra crystals formation. The aim of this work was identification of the nucleotide sequence of small non-coding RNAs, predicted by bioinformatic methods in B. mori polyhedra. The following methods have been used: polymerase chain reaction, agarose gel electrophoresis, the cloning of PCR products, sequencing. There were first determined nucleotide sequences of snc RNA-1 and snc RNA-2 of polyhedrin mRNA complementary regions which are included in B. mori polyhedra. These RNAs have 100% identity with bioinformatic predicted sequences. These results confirmed our bioinformatic approach to the search for small RNAs encoded in B. mori nuclear polyhedrosis virus genome.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Shirina_2_14.pdfBombyx morinuclear polyhedrosis viruspolyhedrinsequencing |
| spellingShingle | T. V. Shirina G. V. Gerashchenko M. T. Bobrovskaja V. I. Kashuba Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus The Ukrainian Biochemical Journal Bombyx mori nuclear polyhedrosis virus polyhedrin sequencing |
| title | Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus |
| title_full | Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus |
| title_fullStr | Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus |
| title_full_unstemmed | Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus |
| title_short | Identification of small RNA in polyhedra of Bombyx mori nuclear polyhedrosis virus |
| title_sort | identification of small rna in polyhedra of bombyx mori nuclear polyhedrosis virus |
| topic | Bombyx mori nuclear polyhedrosis virus polyhedrin sequencing |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Shirina_2_14.pdf |
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