Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity
Microbial photoinactivation using ultraviolet (UV) or visible light can be enhanced by photosensitizers. This study assessed the efficacy of encapsulating a food-grade photosensitizer (curcumin) in surfactant micelles on its water dispersibility, chemical stability, and antimicrobial activity. Stock...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/8/1777 |
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| author | Victor Ryu Silvette Ruiz-Ramirez Piyanan Chuesiang Lynne A. McLandsborough David Julian McClements Maria G. Corradini |
| author_facet | Victor Ryu Silvette Ruiz-Ramirez Piyanan Chuesiang Lynne A. McLandsborough David Julian McClements Maria G. Corradini |
| author_sort | Victor Ryu |
| collection | DOAJ |
| description | Microbial photoinactivation using ultraviolet (UV) or visible light can be enhanced by photosensitizers. This study assessed the efficacy of encapsulating a food-grade photosensitizer (curcumin) in surfactant micelles on its water dispersibility, chemical stability, and antimicrobial activity. Stock curcumin-surfactant solutions were prepared with Surfynol 465 (S465) or Tween 80 (T80) (5 mM sodium citrate buffer). The antimicrobial activity of curcumin-loaded surfactant solutions was determined by monitoring the inactivation of <i>Escherichia coli</i> O157: H7 and <i>Listeria innocua</i> after 5-min irradiation with UV-A light (λ = 365 nm). The solutions mixed with the bacterial suspensions contained 1 µM curcumin and each surfactant below, near, and above their critical micelle concentrations (CMCs). The addition of surfactants at any level to the curcumin solution enhanced its dispersibility, stability, and efficacy as a photosensitizer, thereby enhancing its antimicrobial activity. Gram-positive bacteria were more susceptible than Gram-negative bacteria when curcumin-loaded micelles were used against them. The photoinactivation efficacy of curcumin-surfactant solutions depended on the pH of the solution (low > high), surfactant type (S465 > T80), and the amount of surfactant present (below CMC ≥ near CMC > above CMC = unencapsulated curcumin). This result suggests that excessive partitioning of curcumin into micelles reduced its ability to interact with microbial cells. Synergistic antimicrobial activity was observed when S465 was present below or near the CMC with curcumin at pH 3.5, which could be attributed to a more effective interaction of the photosensitizer with the cell membranes as supported by the fluorescence lifetime micrographs. The use of a micelle-based delivery system facilitates adsorption and generation of reactive oxygen species in the immediate environment of the microbial cell, enhancing photoinactivation. |
| first_indexed | 2024-03-10T08:48:45Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T08:48:45Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-71da8a8a1594440289a5072f744dc7da2023-11-22T07:38:38ZengMDPI AGFoods2304-81582021-07-01108177710.3390/foods10081777Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation CapacityVictor Ryu0Silvette Ruiz-Ramirez1Piyanan Chuesiang2Lynne A. McLandsborough3David Julian McClements4Maria G. Corradini5Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USADepartment of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USADepartment of Food Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USADepartment of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USAFood Science Department and Arrell Food Institute, University of Guelph, Guelph, ON N1G 2W1, CanadaMicrobial photoinactivation using ultraviolet (UV) or visible light can be enhanced by photosensitizers. This study assessed the efficacy of encapsulating a food-grade photosensitizer (curcumin) in surfactant micelles on its water dispersibility, chemical stability, and antimicrobial activity. Stock curcumin-surfactant solutions were prepared with Surfynol 465 (S465) or Tween 80 (T80) (5 mM sodium citrate buffer). The antimicrobial activity of curcumin-loaded surfactant solutions was determined by monitoring the inactivation of <i>Escherichia coli</i> O157: H7 and <i>Listeria innocua</i> after 5-min irradiation with UV-A light (λ = 365 nm). The solutions mixed with the bacterial suspensions contained 1 µM curcumin and each surfactant below, near, and above their critical micelle concentrations (CMCs). The addition of surfactants at any level to the curcumin solution enhanced its dispersibility, stability, and efficacy as a photosensitizer, thereby enhancing its antimicrobial activity. Gram-positive bacteria were more susceptible than Gram-negative bacteria when curcumin-loaded micelles were used against them. The photoinactivation efficacy of curcumin-surfactant solutions depended on the pH of the solution (low > high), surfactant type (S465 > T80), and the amount of surfactant present (below CMC ≥ near CMC > above CMC = unencapsulated curcumin). This result suggests that excessive partitioning of curcumin into micelles reduced its ability to interact with microbial cells. Synergistic antimicrobial activity was observed when S465 was present below or near the CMC with curcumin at pH 3.5, which could be attributed to a more effective interaction of the photosensitizer with the cell membranes as supported by the fluorescence lifetime micrographs. The use of a micelle-based delivery system facilitates adsorption and generation of reactive oxygen species in the immediate environment of the microbial cell, enhancing photoinactivation.https://www.mdpi.com/2304-8158/10/8/1777microbial photoinactivationcurcuminmicellephotosensitizerreactive oxygen speciessinglet oxygen (<sup>1</sup>O<sub>2</sub>) |
| spellingShingle | Victor Ryu Silvette Ruiz-Ramirez Piyanan Chuesiang Lynne A. McLandsborough David Julian McClements Maria G. Corradini Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity Foods microbial photoinactivation curcumin micelle photosensitizer reactive oxygen species singlet oxygen (<sup>1</sup>O<sub>2</sub>) |
| title | Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity |
| title_full | Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity |
| title_fullStr | Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity |
| title_full_unstemmed | Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity |
| title_short | Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity |
| title_sort | use of micellar delivery systems to enhance curcumin s stability and microbial photoinactivation capacity |
| topic | microbial photoinactivation curcumin micelle photosensitizer reactive oxygen species singlet oxygen (<sup>1</sup>O<sub>2</sub>) |
| url | https://www.mdpi.com/2304-8158/10/8/1777 |
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