The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative
A novel polysaccharide (MFP4P) was isolated from mulberry fruits (Morus alba L.) pulp using a combination of DEAE-Sepharose fast-flow and Sephadex G-100 chromatographic columns. Structural characterization revealed that MFP4P was composed of arabinose, rhamnose, galactose, glucose and galacturonic a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619300088 |
| _version_ | 1818728375910400000 |
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| author | Ping-Ping Wang Qiang Huang Chun Chen Li-Jun You Rui Hai Liu Zhi-Gang Luo Mou-Ming Zhao Xiong Fu |
| author_facet | Ping-Ping Wang Qiang Huang Chun Chen Li-Jun You Rui Hai Liu Zhi-Gang Luo Mou-Ming Zhao Xiong Fu |
| author_sort | Ping-Ping Wang |
| collection | DOAJ |
| description | A novel polysaccharide (MFP4P) was isolated from mulberry fruits (Morus alba L.) pulp using a combination of DEAE-Sepharose fast-flow and Sephadex G-100 chromatographic columns. Structural characterization revealed that MFP4P was composed of arabinose, rhamnose, galactose, glucose and galacturonic acid with a molecular weight of 198.2 kDa. The linkage types of MFP4P were proved to be (1 → 6)-linked α-D-Glc, (1 → 2)-linked α-L-Rha, (1 → 3)-linked α-D-Gal, (1 → 3)-linked β-L-Rha and (1 → )-linked α-L-Ara. The MFP4P showed good antioxidant activity with an oxygen radical absorbance capacity value of 121.8 ± 16.5 μM trolox equivalent/g. In addition, the MFP4P promoted pancreatic cell proliferation, increased insulin secretion, and enhanced glucose consumption. In the non-toxic range, the polysaccharide and zinc complexes (MFP4P-Zn) exhibited higher antioxidant and hypoglycemic activities than MFP4P at the same concentration. In vivo hypoglycemic study, MFP4P and MFP4P-Zn both showed decreased blood glucose level and glucose tolerance compared with the model control group. |
| first_indexed | 2024-12-17T22:29:00Z |
| format | Article |
| id | doaj.art-71da8d065b894797892ad562cff6c607 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T22:29:00Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-71da8d065b894797892ad562cff6c6072022-12-21T21:30:16ZengElsevierJournal of Functional Foods1756-46462019-03-01546473The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivativePing-Ping Wang0Qiang Huang1Chun Chen2Li-Jun You3Rui Hai Liu4Zhi-Gang Luo5Mou-Ming Zhao6Xiong Fu7School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, ChinaSchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China; Corresponding authors at: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USASchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, ChinaSchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China; Corresponding authors at: School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.A novel polysaccharide (MFP4P) was isolated from mulberry fruits (Morus alba L.) pulp using a combination of DEAE-Sepharose fast-flow and Sephadex G-100 chromatographic columns. Structural characterization revealed that MFP4P was composed of arabinose, rhamnose, galactose, glucose and galacturonic acid with a molecular weight of 198.2 kDa. The linkage types of MFP4P were proved to be (1 → 6)-linked α-D-Glc, (1 → 2)-linked α-L-Rha, (1 → 3)-linked α-D-Gal, (1 → 3)-linked β-L-Rha and (1 → )-linked α-L-Ara. The MFP4P showed good antioxidant activity with an oxygen radical absorbance capacity value of 121.8 ± 16.5 μM trolox equivalent/g. In addition, the MFP4P promoted pancreatic cell proliferation, increased insulin secretion, and enhanced glucose consumption. In the non-toxic range, the polysaccharide and zinc complexes (MFP4P-Zn) exhibited higher antioxidant and hypoglycemic activities than MFP4P at the same concentration. In vivo hypoglycemic study, MFP4P and MFP4P-Zn both showed decreased blood glucose level and glucose tolerance compared with the model control group.http://www.sciencedirect.com/science/article/pii/S1756464619300088Murus alba L.PolysaccharideStructuralZincAntioxidantHypoglycemic |
| spellingShingle | Ping-Ping Wang Qiang Huang Chun Chen Li-Jun You Rui Hai Liu Zhi-Gang Luo Mou-Ming Zhao Xiong Fu The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative Journal of Functional Foods Murus alba L. Polysaccharide Structural Zinc Antioxidant Hypoglycemic |
| title | The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative |
| title_full | The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative |
| title_fullStr | The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative |
| title_full_unstemmed | The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative |
| title_short | The chemical structure and biological activities of a novel polysaccharide obtained from Fructus Mori and its zinc derivative |
| title_sort | chemical structure and biological activities of a novel polysaccharide obtained from fructus mori and its zinc derivative |
| topic | Murus alba L. Polysaccharide Structural Zinc Antioxidant Hypoglycemic |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619300088 |
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