Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-01-01
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| Series: | Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001 |
| _version_ | 1819195729348919296 |
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| author | STEFAN SCHREIER WANNAPONG TRIAMPO GALAYANEE DOUNGCHAWEE DARAPOND TRIAMPO SUDARAT CHADSUTHI |
| author_facet | STEFAN SCHREIER WANNAPONG TRIAMPO GALAYANEE DOUNGCHAWEE DARAPOND TRIAMPO SUDARAT CHADSUTHI |
| author_sort | STEFAN SCHREIER |
| collection | DOAJ |
| description | Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research. |
| first_indexed | 2024-12-23T02:17:23Z |
| format | Article |
| id | doaj.art-71db998e7ddc4bbfbdef2cb2a9f5ff16 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-23T02:17:23Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-71db998e7ddc4bbfbdef2cb2a9f5ff162022-12-21T18:03:37ZengBMCBiological Research0716-97600717-62872009-01-01421512Leptospirosis research: fast, easy and reliable enumeration of mobile leptospiresSTEFAN SCHREIERWANNAPONG TRIAMPOGALAYANEE DOUNGCHAWEEDARAPOND TRIAMPOSUDARAT CHADSUTHILeptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001leptospirosisleptospiraenumerationspectroscopyindirect counting |
| spellingShingle | STEFAN SCHREIER WANNAPONG TRIAMPO GALAYANEE DOUNGCHAWEE DARAPOND TRIAMPO SUDARAT CHADSUTHI Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires Biological Research leptospirosis leptospira enumeration spectroscopy indirect counting |
| title | Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires |
| title_full | Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires |
| title_fullStr | Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires |
| title_full_unstemmed | Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires |
| title_short | Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires |
| title_sort | leptospirosis research fast easy and reliable enumeration of mobile leptospires |
| topic | leptospirosis leptospira enumeration spectroscopy indirect counting |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001 |
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