The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility
In this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in d...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/24/9035 |
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| author | Yung-Chieh Cho Hsiao-Ting Huang Wen-Chien Lan Mao-Suan Huang Takashi Saito Bai-Hung Huang Chi-Hsun Tsai Fang-Yu Fan Keng-Liang Ou |
| author_facet | Yung-Chieh Cho Hsiao-Ting Huang Wen-Chien Lan Mao-Suan Huang Takashi Saito Bai-Hung Huang Chi-Hsun Tsai Fang-Yu Fan Keng-Liang Ou |
| author_sort | Yung-Chieh Cho |
| collection | DOAJ |
| description | In this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in different concentrations of the 3BE hydrogel (0%, 0.05%, 0.1%, 0.25%, and 0.5%, respectively). The cell morphology and differentiation potential of HFMSCs were observed through optical microscopy, and the cell viability was investigated via Live/Dead Kit and Cell Counting Kit-8 assay. Analytical results showed that HFMSC can differentiate into adipogenic, chondrogenic, and osteogenic lineages. The HFMSC and Swiss 3T3 cells would properly assemble into a spherical shape as cultured with the 3BE hydrogel. Most importantly, cell viability could be maintained above 70%. The formation of spheroid structures of cells within this hydrogel is predicted to promote cell differentiation potentials of HFMSC that benefit in generating functional adipocytes, chondrocytes, and osteoblasts. Therefore, these findings demonstrate that the 3BE hydrogel has great potential as a three-dimensional cell culture scaffold for tissue engineering applications. |
| first_indexed | 2024-03-10T13:59:07Z |
| format | Article |
| id | doaj.art-71dc07d394384b1e8fe048065e2af21d |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T13:59:07Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-71dc07d394384b1e8fe048065e2af21d2023-11-21T01:18:35ZengMDPI AGApplied Sciences2076-34172020-12-011024903510.3390/app10249035The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and BiocompatibilityYung-Chieh Cho0Hsiao-Ting Huang1Wen-Chien Lan2Mao-Suan Huang3Takashi Saito4Bai-Hung Huang5Chi-Hsun Tsai6Fang-Yu Fan7Keng-Liang Ou8School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDivision of Oral Rehabilitation and Center of Pediatric Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, TaiwanDepartment of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, TaiwanDepartment of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, TaiwanDivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, JapanBiomedical Technology R & D Center, China Medical University Hospital, Taichung 404, TaiwanDivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, JapanBiomedical Technology R & D Center, China Medical University Hospital, Taichung 404, TaiwanBiomedical Technology R & D Center, China Medical University Hospital, Taichung 404, TaiwanIn this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in different concentrations of the 3BE hydrogel (0%, 0.05%, 0.1%, 0.25%, and 0.5%, respectively). The cell morphology and differentiation potential of HFMSCs were observed through optical microscopy, and the cell viability was investigated via Live/Dead Kit and Cell Counting Kit-8 assay. Analytical results showed that HFMSC can differentiate into adipogenic, chondrogenic, and osteogenic lineages. The HFMSC and Swiss 3T3 cells would properly assemble into a spherical shape as cultured with the 3BE hydrogel. Most importantly, cell viability could be maintained above 70%. The formation of spheroid structures of cells within this hydrogel is predicted to promote cell differentiation potentials of HFMSC that benefit in generating functional adipocytes, chondrocytes, and osteoblasts. Therefore, these findings demonstrate that the 3BE hydrogel has great potential as a three-dimensional cell culture scaffold for tissue engineering applications.https://www.mdpi.com/2076-3417/10/24/9035pluronic F127tripeptide Arg-Gly-Aspspheroidshair folliclemesenchymal stem cells |
| spellingShingle | Yung-Chieh Cho Hsiao-Ting Huang Wen-Chien Lan Mao-Suan Huang Takashi Saito Bai-Hung Huang Chi-Hsun Tsai Fang-Yu Fan Keng-Liang Ou The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility Applied Sciences pluronic F127 tripeptide Arg-Gly-Asp spheroids hair follicle mesenchymal stem cells |
| title | The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility |
| title_full | The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility |
| title_fullStr | The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility |
| title_full_unstemmed | The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility |
| title_short | The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility |
| title_sort | potential of a tailored biomimetic hydrogel for in vitro cell culture applications characterization and biocompatibility |
| topic | pluronic F127 tripeptide Arg-Gly-Asp spheroids hair follicle mesenchymal stem cells |
| url | https://www.mdpi.com/2076-3417/10/24/9035 |
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