Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro
The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrat...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/22/11/1918 |
| _version_ | 1819212841059614720 |
|---|---|
| author | Xiaona Zhao Yuge Wang Peng Yan Guodong Cheng Cheng Wang Na Geng Xuepeng Wang Jianzhu Liu |
| author_facet | Xiaona Zhao Yuge Wang Peng Yan Guodong Cheng Cheng Wang Na Geng Xuepeng Wang Jianzhu Liu |
| author_sort | Xiaona Zhao |
| collection | DOAJ |
| description | The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrations of PGPSs (PGPStc, PGPS60c, PGPS80c, and PGPStp) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPStc groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPStc and PGPS60c were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4+ and CD8+ T cells. At most time points, PGPStc could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments. |
| first_indexed | 2024-12-23T06:49:22Z |
| format | Article |
| id | doaj.art-71e0a90caddd4d5ab44a7bc4c0457b7f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T06:49:22Z |
| publishDate | 2017-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-71e0a90caddd4d5ab44a7bc4c0457b7f2022-12-21T17:56:29ZengMDPI AGMolecules1420-30492017-11-012211191810.3390/molecules22111918molecules22111918Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In VitroXiaona Zhao0Yuge Wang1Peng Yan2Guodong Cheng3Cheng Wang4Na Geng5Xuepeng Wang6Jianzhu Liu7College of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaResearch Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, ChinaCollege of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaThe study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrations of PGPSs (PGPStc, PGPS60c, PGPS80c, and PGPStp) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPStc groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPStc and PGPS60c were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4+ and CD8+ T cells. At most time points, PGPStc could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments.https://www.mdpi.com/1420-3049/22/11/1918Platycodon grandiflorumpolysaccharideslymphocyte proliferationcell cycleimmunity-enhancing |
| spellingShingle | Xiaona Zhao Yuge Wang Peng Yan Guodong Cheng Cheng Wang Na Geng Xuepeng Wang Jianzhu Liu Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro Molecules Platycodon grandiflorum polysaccharides lymphocyte proliferation cell cycle immunity-enhancing |
| title | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
| title_full | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
| title_fullStr | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
| title_full_unstemmed | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
| title_short | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
| title_sort | effects of polysaccharides from platycodon grandiflorum on immunity enhancing activity in vitro |
| topic | Platycodon grandiflorum polysaccharides lymphocyte proliferation cell cycle immunity-enhancing |
| url | https://www.mdpi.com/1420-3049/22/11/1918 |
| work_keys_str_mv | AT xiaonazhao effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT yugewang effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT pengyan effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT guodongcheng effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT chengwang effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT nageng effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT xuepengwang effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT jianzhuliu effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro |