Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.)
Common vetch (<i>Vicia sativa</i> L.) is an important annual diploid leguminous forage. In the present study, transcriptomic profiling in common vetch in response to salt stress was conducted using a salt-tolerant line (460) and a salt-sensitive line (429). The common responses in common...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/5/714 |
| _version_ | 1827319449764495360 |
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| author | Yanmei Sun Na Zhao Hongjian Sun Shan Xu Yiwen Lu Haojie Xi Zhenfei Guo Haifan Shi |
| author_facet | Yanmei Sun Na Zhao Hongjian Sun Shan Xu Yiwen Lu Haojie Xi Zhenfei Guo Haifan Shi |
| author_sort | Yanmei Sun |
| collection | DOAJ |
| description | Common vetch (<i>Vicia sativa</i> L.) is an important annual diploid leguminous forage. In the present study, transcriptomic profiling in common vetch in response to salt stress was conducted using a salt-tolerant line (460) and a salt-sensitive line (429). The common responses in common vetch and the specific responses associated with salt tolerance in 460 were analyzed. Several KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including plant hormone and MAPK (mitogen-activated protein kinase) signaling, galactose metabolism, and phenylpropanoid phenylpropane biosynthesis, were enriched in both lines, though some differentially expressed genes (DEGs) showed distinct expression patterns. The roots in 460 showed higher levels of lignin than in 429. α-linolenic acid metabolism, carotenoid biosynthesis, the photosynthesis-antenna pathway, and starch and sucrose metabolism pathways were specifically enriched in salt-tolerant line 460, with higher levels of accumulated soluble sugars in the leaves. In addition, higher transcript levels of genes involved in ion homeostasis and reactive oxygen species (ROS) scavenging were observed in 460 than in 429 in response to salt stress. The transcriptomic analysis in common vetch in response to salt stress provides useful clues for further investigations on salt tolerance mechanism in the future. |
| first_indexed | 2024-04-25T00:22:10Z |
| format | Article |
| id | doaj.art-71e0b7fbd08a4dfd8b54678a5a868f4e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-25T00:22:10Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-71e0b7fbd08a4dfd8b54678a5a868f4e2024-03-12T16:53:05ZengMDPI AGPlants2223-77472024-03-0113571410.3390/plants13050714Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.)Yanmei Sun0Na Zhao1Hongjian Sun2Shan Xu3Yiwen Lu4Haojie Xi5Zhenfei Guo6Haifan Shi7Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, ChinaCommon vetch (<i>Vicia sativa</i> L.) is an important annual diploid leguminous forage. In the present study, transcriptomic profiling in common vetch in response to salt stress was conducted using a salt-tolerant line (460) and a salt-sensitive line (429). The common responses in common vetch and the specific responses associated with salt tolerance in 460 were analyzed. Several KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including plant hormone and MAPK (mitogen-activated protein kinase) signaling, galactose metabolism, and phenylpropanoid phenylpropane biosynthesis, were enriched in both lines, though some differentially expressed genes (DEGs) showed distinct expression patterns. The roots in 460 showed higher levels of lignin than in 429. α-linolenic acid metabolism, carotenoid biosynthesis, the photosynthesis-antenna pathway, and starch and sucrose metabolism pathways were specifically enriched in salt-tolerant line 460, with higher levels of accumulated soluble sugars in the leaves. In addition, higher transcript levels of genes involved in ion homeostasis and reactive oxygen species (ROS) scavenging were observed in 460 than in 429 in response to salt stress. The transcriptomic analysis in common vetch in response to salt stress provides useful clues for further investigations on salt tolerance mechanism in the future.https://www.mdpi.com/2223-7747/13/5/714common vetchKEGG pathwaylignin biosynthesisRNA-seqsalt tolerancesoluble sugars |
| spellingShingle | Yanmei Sun Na Zhao Hongjian Sun Shan Xu Yiwen Lu Haojie Xi Zhenfei Guo Haifan Shi Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) Plants common vetch KEGG pathway lignin biosynthesis RNA-seq salt tolerance soluble sugars |
| title | Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) |
| title_full | Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) |
| title_fullStr | Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) |
| title_full_unstemmed | Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) |
| title_short | Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (<i>Vicia sativa</i> L.) |
| title_sort | transcriptome profiling reveals molecular responses to salt stress in common vetch i vicia sativa i l |
| topic | common vetch KEGG pathway lignin biosynthesis RNA-seq salt tolerance soluble sugars |
| url | https://www.mdpi.com/2223-7747/13/5/714 |
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