Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation
The aim of this work was to investigate of biocompatibility of polymeric implants modified with silver nanoparticles (AgNPs). Middle ear prostheses (otoimplants) made of the (poly)acrylonitrile butadiene styrene (ABS) and ABS modified with silver nanoparticles were prepared through extrusion and inj...
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| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/10/764 |
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| author | Magdalena Ziąbka Elżbieta Menaszek Jacek Tarasiuk Sebastian Wroński |
| author_facet | Magdalena Ziąbka Elżbieta Menaszek Jacek Tarasiuk Sebastian Wroński |
| author_sort | Magdalena Ziąbka |
| collection | DOAJ |
| description | The aim of this work was to investigate of biocompatibility of polymeric implants modified with silver nanoparticles (AgNPs). Middle ear prostheses (otoimplants) made of the (poly)acrylonitrile butadiene styrene (ABS) and ABS modified with silver nanoparticles were prepared through extrusion and injection moulding process. The obtained prostheses were characterized by SEM-EDX, micro-CT and mechanical tests, confirming their proper shape, good AgNPs homogenization and mechanical parameters stability. The biocompatibility of the implants was evaluated in vivo on rats, after 4, 12, 24 and 48 weeks of implantation. The tissue-healing process and cytotoxicity of the implants were evaluated on the basis of microscopic observations of the materials morphology after histochemical staining with cytochrome c oxidase (OCC) and acid phosphatase (AP), as well as via micro-tomography (ex vivo). The in vivo studies confirmed biocompatibility of the implants in the surrounding tissue environment. Both the pure ABS and nanosilver-modified ABS implants exhibited a distinct decrease in the area of granulation tissue which was replaced with the regenerating muscle tissue. Moreover, a slightly smaller area of granulation tissue was observed in the surroundings of the silver-doped prosthesis than in the case of pure ABS prosthesis. The kinetics of silver ions releasing from implants was investigated by ICP-MS spectrometry. The measurement confirmed that concentration of the silver ions increased within the implant’s immersion period. Our results showed that middle ear implant with the nanoscale modification is biocompatible and might be used in ossicular reconstruction. |
| first_indexed | 2024-12-13T16:02:22Z |
| format | Article |
| id | doaj.art-71e5026cd49441b18a417a74761b5f54 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-13T16:02:22Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-71e5026cd49441b18a417a74761b5f542022-12-21T23:39:08ZengMDPI AGNanomaterials2079-49912018-09-0181076410.3390/nano8100764nano8100764Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo EvaluationMagdalena Ziąbka0Elżbieta Menaszek1Jacek Tarasiuk2Sebastian Wroński3Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, PolandDepartment of Cytobiology, Collegium Medicum, Faculty of Pharmacy, UJ Jagiellonian University, 30-001 Krakow, PolandDepartment of Condensed Matter Physics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Krakow, PolandDepartment of Condensed Matter Physics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Krakow, PolandThe aim of this work was to investigate of biocompatibility of polymeric implants modified with silver nanoparticles (AgNPs). Middle ear prostheses (otoimplants) made of the (poly)acrylonitrile butadiene styrene (ABS) and ABS modified with silver nanoparticles were prepared through extrusion and injection moulding process. The obtained prostheses were characterized by SEM-EDX, micro-CT and mechanical tests, confirming their proper shape, good AgNPs homogenization and mechanical parameters stability. The biocompatibility of the implants was evaluated in vivo on rats, after 4, 12, 24 and 48 weeks of implantation. The tissue-healing process and cytotoxicity of the implants were evaluated on the basis of microscopic observations of the materials morphology after histochemical staining with cytochrome c oxidase (OCC) and acid phosphatase (AP), as well as via micro-tomography (ex vivo). The in vivo studies confirmed biocompatibility of the implants in the surrounding tissue environment. Both the pure ABS and nanosilver-modified ABS implants exhibited a distinct decrease in the area of granulation tissue which was replaced with the regenerating muscle tissue. Moreover, a slightly smaller area of granulation tissue was observed in the surroundings of the silver-doped prosthesis than in the case of pure ABS prosthesis. The kinetics of silver ions releasing from implants was investigated by ICP-MS spectrometry. The measurement confirmed that concentration of the silver ions increased within the implant’s immersion period. Our results showed that middle ear implant with the nanoscale modification is biocompatible and might be used in ossicular reconstruction.http://www.mdpi.com/2079-4991/8/10/764nanocompositesmedical devicesmiddle ear prosthesissilver nanoparticlesbiocompatibilitythermoplastic polymer |
| spellingShingle | Magdalena Ziąbka Elżbieta Menaszek Jacek Tarasiuk Sebastian Wroński Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation Nanomaterials nanocomposites medical devices middle ear prosthesis silver nanoparticles biocompatibility thermoplastic polymer |
| title | Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation |
| title_full | Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation |
| title_fullStr | Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation |
| title_full_unstemmed | Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation |
| title_short | Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation |
| title_sort | biocompatible nanocomposite implant with silver nanoparticles for otology in vivo evaluation |
| topic | nanocomposites medical devices middle ear prosthesis silver nanoparticles biocompatibility thermoplastic polymer |
| url | http://www.mdpi.com/2079-4991/8/10/764 |
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