Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L.
Natural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from <i>Myrtus communis</i> (PEMC) on acute and chronic leukemia. Accord...
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MDPI AG
2022-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/23/5055 |
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| author | Hamza Mechchate Carlos Eduardo de Castro Alves Imane Es-safi Amal Amaghnouje Fatima Zahra Jawhari Regiane Costa de Oliveira Alice de Freitas Gomes Raffaele Conte Gemilson Soares Pontes Dalila Bousta Andriy Grafov |
| author_facet | Hamza Mechchate Carlos Eduardo de Castro Alves Imane Es-safi Amal Amaghnouje Fatima Zahra Jawhari Regiane Costa de Oliveira Alice de Freitas Gomes Raffaele Conte Gemilson Soares Pontes Dalila Bousta Andriy Grafov |
| author_sort | Hamza Mechchate |
| collection | DOAJ |
| description | Natural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from <i>Myrtus communis</i> (PEMC) on acute and chronic leukemia. According to the UHPLC-MS<sup>n</sup>, the fraction is rich in flavonoids. Protective activity of the PEMC was assessed by evaluating the antioxidant, anti-inflammatory, wound healing, and hemolysis potential in a series of in vivo and in vitro assays, while the therapeutic approach consisted of the evaluation of cytotoxic activity of the PEMC against HL60 and K562 leukemia cell lines. Safety of the fraction was also evaluated on a non-cancerous Vero cell line and by an acute toxicity test performed in mice. The PEMC demonstrated a significant anti-inflammatory and healing potential. The activities found at the dose of 100 mg/kg were better than those observed using a reference drug. The PEMC demonstrated a significant antioxidant effect and a specific cytotoxicity towards HL60 (IC<sub>50</sub> = 19.87 µM) and K562 (IC<sub>50</sub> = 29.64 µM) cell lines being non-toxic to the Vero cell line. No hemolytic activity was observed in vitro and no toxicity effect was found in mice. Thus, the PEMC has a pharmacological potential as both preventive and therapeutic agent. However, further research is necessary to propose its mechanism of action. |
| first_indexed | 2024-03-09T17:37:44Z |
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| id | doaj.art-71e5d2b5787a4501837db26a82e029e9 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T17:37:44Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-71e5d2b5787a4501837db26a82e029e92023-11-24T11:51:23ZengMDPI AGNutrients2072-66432022-11-011423505510.3390/nu14235055Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L.Hamza Mechchate0Carlos Eduardo de Castro Alves1Imane Es-safi2Amal Amaghnouje3Fatima Zahra Jawhari4Regiane Costa de Oliveira5Alice de Freitas Gomes6Raffaele Conte7Gemilson Soares Pontes8Dalila Bousta9Andriy Grafov10Laboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, MoroccoPost-Graduate Program in Basic and Applied Immunology, Institute of Biological Sciences, Federal University of Amazonas, Manaus 69077-000, AM, BrazilLaboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, MoroccoLaboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, MoroccoLaboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, MoroccoPost-Graduate Program in Hematology, The State University of Amazonas, Foundation of Hematology and Hemotherapy of Amazonas, Manaus 69050-010, AM, BrazilPost-Graduate Program in Hematology, The State University of Amazonas, Foundation of Hematology and Hemotherapy of Amazonas, Manaus 69050-010, AM, BrazilResearch Institute on Terrestrial Ecosystems (IRET)—CNR, Via Pietro Castellino 111, 80131 Naples, ItalyPost-Graduate Program in Basic and Applied Immunology, Institute of Biological Sciences, Federal University of Amazonas, Manaus 69077-000, AM, BrazilLaboratory of Biotechnology, Environment, Agrifood, and Health, University of Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, MoroccoMaterials Chemistry Division, Department of Chemistry, University of Helsinki, A.I. Virtasen aukio 1, 00560 Helsinki, FinlandNatural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from <i>Myrtus communis</i> (PEMC) on acute and chronic leukemia. According to the UHPLC-MS<sup>n</sup>, the fraction is rich in flavonoids. Protective activity of the PEMC was assessed by evaluating the antioxidant, anti-inflammatory, wound healing, and hemolysis potential in a series of in vivo and in vitro assays, while the therapeutic approach consisted of the evaluation of cytotoxic activity of the PEMC against HL60 and K562 leukemia cell lines. Safety of the fraction was also evaluated on a non-cancerous Vero cell line and by an acute toxicity test performed in mice. The PEMC demonstrated a significant anti-inflammatory and healing potential. The activities found at the dose of 100 mg/kg were better than those observed using a reference drug. The PEMC demonstrated a significant antioxidant effect and a specific cytotoxicity towards HL60 (IC<sub>50</sub> = 19.87 µM) and K562 (IC<sub>50</sub> = 29.64 µM) cell lines being non-toxic to the Vero cell line. No hemolytic activity was observed in vitro and no toxicity effect was found in mice. Thus, the PEMC has a pharmacological potential as both preventive and therapeutic agent. However, further research is necessary to propose its mechanism of action.https://www.mdpi.com/2072-6643/14/23/5055anticancerHL60K562wound healing activitycarrageenan-induced edema testhemolysis test |
| spellingShingle | Hamza Mechchate Carlos Eduardo de Castro Alves Imane Es-safi Amal Amaghnouje Fatima Zahra Jawhari Regiane Costa de Oliveira Alice de Freitas Gomes Raffaele Conte Gemilson Soares Pontes Dalila Bousta Andriy Grafov Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. Nutrients anticancer HL60 K562 wound healing activity carrageenan-induced edema test hemolysis test |
| title | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. |
| title_full | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. |
| title_fullStr | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. |
| title_full_unstemmed | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. |
| title_short | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of <i>Myrtus communis</i> L. |
| title_sort | antileukemic antioxidant anti inflammatory and healing activities induced by a polyphenol enriched fraction extracted from leaves of i myrtus communis i l |
| topic | anticancer HL60 K562 wound healing activity carrageenan-induced edema test hemolysis test |
| url | https://www.mdpi.com/2072-6643/14/23/5055 |
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