An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma
<p>Abstract</p> <p>Background</p> <p>The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental and clinical studies. The vital dye staining method applied in the detection of anti-HIV-1 neutralizing antibody has been used...
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| Format: | Članak |
| Jezik: | English |
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BMC
2003-12-01
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| Serija: | BMC Infectious Diseases |
| Teme: | |
| Online pristup: | http://www.biomedcentral.com/1471-2334/3/30 |
| _version_ | 1830450403094822912 |
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| author | Chen Yunyun Zhang Chiyu Ben Kunlong |
| author_facet | Chen Yunyun Zhang Chiyu Ben Kunlong |
| author_sort | Chen Yunyun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental and clinical studies. The vital dye staining method applied in the detection of anti-HIV-1 neutralizing antibody has been used in many laboratories. However, the unknown factor(s) in sera or plasma affected cell growth and caused protection when the tested sera or plasma was continuously maintained in cell culture. In addition, the poor solubility of neutral red in medium (such as RPMI-1640) also limited the use of this assay.</p> <p>Methods</p> <p>In this study, human T cell line C8166 was used as host cells, and 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) instead of neutral red was used as vital dye. In order to avoid the effect of the unknown factor(s), the tested sera or plasma was removed by a washout procedure after initial 3–6 h culture in the assay.</p> <p>Result</p> <p>This new assay eliminated the effect of the tested sera or plasma on cell growth, improved the reliability of detection of anti-HIV-1 neutralizing antibody, and showed excellent agreement with the p24 antigen method.</p> <p>Conclusion</p> <p>The results suggest that the improved assay is relatively simple, highly duplicable, cost-effective, and well reliable for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma.</p> |
| first_indexed | 2024-12-21T08:14:42Z |
| format | Article |
| id | doaj.art-71e87b2bcdfc402783e465a002a32e44 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-21T08:14:42Z |
| publishDate | 2003-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-71e87b2bcdfc402783e465a002a32e442022-12-21T19:10:35ZengBMCBMC Infectious Diseases1471-23342003-12-01313010.1186/1471-2334-3-30An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasmaChen YunyunZhang ChiyuBen Kunlong<p>Abstract</p> <p>Background</p> <p>The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental and clinical studies. The vital dye staining method applied in the detection of anti-HIV-1 neutralizing antibody has been used in many laboratories. However, the unknown factor(s) in sera or plasma affected cell growth and caused protection when the tested sera or plasma was continuously maintained in cell culture. In addition, the poor solubility of neutral red in medium (such as RPMI-1640) also limited the use of this assay.</p> <p>Methods</p> <p>In this study, human T cell line C8166 was used as host cells, and 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) instead of neutral red was used as vital dye. In order to avoid the effect of the unknown factor(s), the tested sera or plasma was removed by a washout procedure after initial 3–6 h culture in the assay.</p> <p>Result</p> <p>This new assay eliminated the effect of the tested sera or plasma on cell growth, improved the reliability of detection of anti-HIV-1 neutralizing antibody, and showed excellent agreement with the p24 antigen method.</p> <p>Conclusion</p> <p>The results suggest that the improved assay is relatively simple, highly duplicable, cost-effective, and well reliable for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma.</p>http://www.biomedcentral.com/1471-2334/3/30HIV-1neutralizing antibodyMTTmicrotiter neutralization assayserum or plasma |
| spellingShingle | Chen Yunyun Zhang Chiyu Ben Kunlong An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma BMC Infectious Diseases HIV-1 neutralizing antibody MTT microtiter neutralization assay serum or plasma |
| title | An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma |
| title_full | An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma |
| title_fullStr | An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma |
| title_full_unstemmed | An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma |
| title_short | An improved microtiter assay for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma |
| title_sort | improved microtiter assay for evaluating anti hiv 1 neutralizing antibodies from sera or plasma |
| topic | HIV-1 neutralizing antibody MTT microtiter neutralization assay serum or plasma |
| url | http://www.biomedcentral.com/1471-2334/3/30 |
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