Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium
Gynostemma pentaphyllum an important medicinal herb, can absorb high amounts of cadmium (Cd) which can lead to excessive Cd contamination during the production of medicines and tea. Hence, it is crucial to investigate the response mechanism of G. pentaphyllum under Cd stress to develop varieties wit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1265971/full |
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| author | Yunyi Zhou Yunyi Zhou Lixiang Yao Lixiang Yao Xueyan Huang Xueyan Huang Ying Li Ying Li Chunli Wang Chunli Wang Qinfen Huang Qinfen Huang Liying Yu Liying Yu Chunliu Pan Chunliu Pan |
| author_facet | Yunyi Zhou Yunyi Zhou Lixiang Yao Lixiang Yao Xueyan Huang Xueyan Huang Ying Li Ying Li Chunli Wang Chunli Wang Qinfen Huang Qinfen Huang Liying Yu Liying Yu Chunliu Pan Chunliu Pan |
| author_sort | Yunyi Zhou |
| collection | DOAJ |
| description | Gynostemma pentaphyllum an important medicinal herb, can absorb high amounts of cadmium (Cd) which can lead to excessive Cd contamination during the production of medicines and tea. Hence, it is crucial to investigate the response mechanism of G. pentaphyllum under Cd stress to develop varieties with low Cd accumulation and high tolerance. Physiological response analysis, transcriptomics and metabolomics were performed on G. pentaphyllum seedlings exposed to Cd stress. Herein, G. pentaphyllum seedlings could significantly enhance antioxidant enzyme activities (POD, CAT and APX), proline and polysaccharide content subject to Cd stress. Transcriptomics analysis identified the secondary metabolites, carbohydrate metabolism, amino acid metabolism, lipid metabolism, and signal transduction pathways associated with Cd stress, which mainly involved the XTH, EXP and GST genes. Metabolomics analysis identified 126 differentially expressed metabolites, including citric acid, flavonoid and amino acids metabolites, which were accumulated under Cd stress. Multi-omics integrative analysis unraveled that the phenylpropanoid biosynthesis, starch, and sucrose metabolism, alpha-linolenic acid metabolism, and ABC transporter were significantly enriched at the gene and metabolic levels in response to Cd stress in G. pentaphyllum. In conclusion, the genetic regulatory network sheds light on Cd response mechanisms in G. pentaphyllum. |
| first_indexed | 2024-03-11T19:13:08Z |
| format | Article |
| id | doaj.art-71e8f69b34b349de857c5bfc6bafdc09 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T19:13:08Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-71e8f69b34b349de857c5bfc6bafdc092023-10-09T10:25:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-10-011410.3389/fpls.2023.12659711265971Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmiumYunyi Zhou0Yunyi Zhou1Lixiang Yao2Lixiang Yao3Xueyan Huang4Xueyan Huang5Ying Li6Ying Li7Chunli Wang8Chunli Wang9Qinfen Huang10Qinfen Huang11Liying Yu12Liying Yu13Chunliu Pan14Chunliu Pan15Guangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Traditional Chinese Medicine (TCM) Resources General Survey and Data Collection Key Laboratory, the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGynostemma pentaphyllum an important medicinal herb, can absorb high amounts of cadmium (Cd) which can lead to excessive Cd contamination during the production of medicines and tea. Hence, it is crucial to investigate the response mechanism of G. pentaphyllum under Cd stress to develop varieties with low Cd accumulation and high tolerance. Physiological response analysis, transcriptomics and metabolomics were performed on G. pentaphyllum seedlings exposed to Cd stress. Herein, G. pentaphyllum seedlings could significantly enhance antioxidant enzyme activities (POD, CAT and APX), proline and polysaccharide content subject to Cd stress. Transcriptomics analysis identified the secondary metabolites, carbohydrate metabolism, amino acid metabolism, lipid metabolism, and signal transduction pathways associated with Cd stress, which mainly involved the XTH, EXP and GST genes. Metabolomics analysis identified 126 differentially expressed metabolites, including citric acid, flavonoid and amino acids metabolites, which were accumulated under Cd stress. Multi-omics integrative analysis unraveled that the phenylpropanoid biosynthesis, starch, and sucrose metabolism, alpha-linolenic acid metabolism, and ABC transporter were significantly enriched at the gene and metabolic levels in response to Cd stress in G. pentaphyllum. In conclusion, the genetic regulatory network sheds light on Cd response mechanisms in G. pentaphyllum.https://www.frontiersin.org/articles/10.3389/fpls.2023.1265971/fullGynostemma pentaphyllumcadmium tolerancephysiological analysisgene expressionmetabolic level |
| spellingShingle | Yunyi Zhou Yunyi Zhou Lixiang Yao Lixiang Yao Xueyan Huang Xueyan Huang Ying Li Ying Li Chunli Wang Chunli Wang Qinfen Huang Qinfen Huang Liying Yu Liying Yu Chunliu Pan Chunliu Pan Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium Frontiers in Plant Science Gynostemma pentaphyllum cadmium tolerance physiological analysis gene expression metabolic level |
| title | Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium |
| title_full | Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium |
| title_fullStr | Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium |
| title_full_unstemmed | Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium |
| title_short | Transcriptomics and metabolomics association analysis revealed the responses of Gynostemma pentaphyllum to cadmium |
| title_sort | transcriptomics and metabolomics association analysis revealed the responses of gynostemma pentaphyllum to cadmium |
| topic | Gynostemma pentaphyllum cadmium tolerance physiological analysis gene expression metabolic level |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1265971/full |
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