Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS
In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. Th...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2014-09-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015&lng=en&tlng=en |
| _version_ | 1818960888069095424 |
|---|---|
| author | JOANNE M.M. SOUZA STRAHILL BERKOV ALBERDAN S. SANTOS |
| author_facet | JOANNE M.M. SOUZA STRAHILL BERKOV ALBERDAN S. SANTOS |
| author_sort | JOANNE M.M. SOUZA |
| collection | DOAJ |
| description | In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work. |
| first_indexed | 2024-12-20T12:04:41Z |
| format | Article |
| id | doaj.art-71eb3e7266904307ba6222ad381efcaf |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-20T12:04:41Z |
| publishDate | 2014-09-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-71eb3e7266904307ba6222ad381efcaf2022-12-21T19:41:26ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902014-09-018631015102710.1590/0001-3765201420130098S0001-37652014000301015Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MSJOANNE M.M. SOUZASTRAHILL BERKOVALBERDAN S. SANTOSIn this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015&lng=en&tlng=enlipídios anti-inflamatóriosBoerhaavia paniculatacultura de calosperfil lipídicoplanta medicinal |
| spellingShingle | JOANNE M.M. SOUZA STRAHILL BERKOV ALBERDAN S. SANTOS Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS Anais da Academia Brasileira de Ciências lipídios anti-inflamatórios Boerhaavia paniculata cultura de calos perfil lipídico planta medicinal |
| title | Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS |
| title_full | Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS |
| title_fullStr | Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS |
| title_full_unstemmed | Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS |
| title_short | Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS |
| title_sort | improvement of friable callus production of boerhaavia paniculata rich and the investigation of its lipid profile by gc ms |
| topic | lipídios anti-inflamatórios Boerhaavia paniculata cultura de calos perfil lipídico planta medicinal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015&lng=en&tlng=en |
| work_keys_str_mv | AT joannemmsouza improvementoffriablecallusproductionofboerhaaviapaniculatarichandtheinvestigationofitslipidprofilebygcms AT strahillberkov improvementoffriablecallusproductionofboerhaaviapaniculatarichandtheinvestigationofitslipidprofilebygcms AT alberdanssantos improvementoffriablecallusproductionofboerhaaviapaniculatarichandtheinvestigationofitslipidprofilebygcms |