High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts
<i>Periploca aphylla</i> (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and <i>n</i>-b...
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MDPI AG
2021-04-01
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| author | Raha Orfali Shagufta Perveen Hanan Y. Aati Perwez Alam Omar M. Noman Javier Palacios Bayan Salem S. Al-Kurbi Areej M. Al-Taweel Afsar Khan Rashad Mehmood Shabana Iqrar Khan |
| author_facet | Raha Orfali Shagufta Perveen Hanan Y. Aati Perwez Alam Omar M. Noman Javier Palacios Bayan Salem S. Al-Kurbi Areej M. Al-Taweel Afsar Khan Rashad Mehmood Shabana Iqrar Khan |
| author_sort | Raha Orfali |
| collection | DOAJ |
| description | <i>Periploca aphylla</i> (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and <i>n</i>-butanol P-2 were subjected to biological assays to screen peroxisome proliferator-activated receptors (PPARα and PPARγ) agnostic, anti-inflammatory, antioxidant, cytotoxic, and estrogenic activities. In addition, five bioactive secondary metabolites were isolated from the aerial parts of the plant: rutin, chlorogenic acid, caffeic acid, ursolic acid, and stigmasterol. P-1 and P-2 decreased cellular oxidative stress by 47.0% and 62.0%, respectively, compared to the standard drug quercetin, while one of the compounds rutin PA-1 isolated from P-1 extract and significantly decreased cellular oxidative stress by 67.0% compared to quercetin (75.0%). P-1 and P-2 also significantly activated PPARγ agnostic. P-1 and P-2 did not inhibit nuclear factor kappa B and inducible nitric oxide synthase activity and showed no cytotoxic or estergenic effects on four human cancer cell lines. In this study, both extracts were standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC showed sharp and compact bands of rutin (<i>R</i><sub>f</sub> = 0.09), caffeic acid (<i>R</i><sub>f</sub> = 0.25), and chlorogenic acid (<i>R</i><sub>f</sub> = 0.39) scanned at <i>λ</i><sub>max</sub> = 340 nm using the water: methanol (60:40 <i>v</i>/<i>v</i>) mobile phase. At <i>λ</i><sub>max</sub> = 539 nm ursolic acid (<i>R</i><sub>f</sub> = 0.20) and stigmasterol (<i>R</i><sub>f</sub> = 0.48) were scanned using the chloroform: methanol (98:2 <i>v</i>/<i>v</i>) as NP-HPTLC mobile phase. Therefore, the developed RP- and NP-HPTLC systems are a precise, sensitive, and specific analytical tool for the quantification of compounds isolated from PA, which can be used as phytomarkers for taxonomical identification and assessment of PA. |
| first_indexed | 2024-03-10T12:40:40Z |
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| spelling | doaj.art-71ec27308110439d98f52497c28e9ba92023-11-21T13:55:58ZengMDPI AGSeparations2297-87392021-04-01844410.3390/separations8040044High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> ExtractsRaha Orfali0Shagufta Perveen1Hanan Y. Aati2Perwez Alam3Omar M. Noman4Javier Palacios5Bayan Salem S. Al-Kurbi6Areej M. Al-Taweel7Afsar Khan8Rashad Mehmood9Shabana Iqrar Khan10Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaLaboratorio de Bioquímica Aplicada, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique 1110939, ChileDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Chemistry, Abbottabad Campus, COMSATS University Islamabad, Abbottabad 22060, PakistanDivision of Science and Technology, Department of Chemistry, University of Education, Township, Lahore 54770, PakistanNational Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS 38677, USA<i>Periploca aphylla</i> (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and <i>n</i>-butanol P-2 were subjected to biological assays to screen peroxisome proliferator-activated receptors (PPARα and PPARγ) agnostic, anti-inflammatory, antioxidant, cytotoxic, and estrogenic activities. In addition, five bioactive secondary metabolites were isolated from the aerial parts of the plant: rutin, chlorogenic acid, caffeic acid, ursolic acid, and stigmasterol. P-1 and P-2 decreased cellular oxidative stress by 47.0% and 62.0%, respectively, compared to the standard drug quercetin, while one of the compounds rutin PA-1 isolated from P-1 extract and significantly decreased cellular oxidative stress by 67.0% compared to quercetin (75.0%). P-1 and P-2 also significantly activated PPARγ agnostic. P-1 and P-2 did not inhibit nuclear factor kappa B and inducible nitric oxide synthase activity and showed no cytotoxic or estergenic effects on four human cancer cell lines. In this study, both extracts were standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC showed sharp and compact bands of rutin (<i>R</i><sub>f</sub> = 0.09), caffeic acid (<i>R</i><sub>f</sub> = 0.25), and chlorogenic acid (<i>R</i><sub>f</sub> = 0.39) scanned at <i>λ</i><sub>max</sub> = 340 nm using the water: methanol (60:40 <i>v</i>/<i>v</i>) mobile phase. At <i>λ</i><sub>max</sub> = 539 nm ursolic acid (<i>R</i><sub>f</sub> = 0.20) and stigmasterol (<i>R</i><sub>f</sub> = 0.48) were scanned using the chloroform: methanol (98:2 <i>v</i>/<i>v</i>) as NP-HPTLC mobile phase. Therefore, the developed RP- and NP-HPTLC systems are a precise, sensitive, and specific analytical tool for the quantification of compounds isolated from PA, which can be used as phytomarkers for taxonomical identification and assessment of PA.https://www.mdpi.com/2297-8739/8/4/44<i>Periploca aphylla</i>Reverse phase (RP-)Normal Phase (NP-)HPTLCantioxidantPPARγ |
| spellingShingle | Raha Orfali Shagufta Perveen Hanan Y. Aati Perwez Alam Omar M. Noman Javier Palacios Bayan Salem S. Al-Kurbi Areej M. Al-Taweel Afsar Khan Rashad Mehmood Shabana Iqrar Khan High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts Separations <i>Periploca aphylla</i> Reverse phase (RP-) Normal Phase (NP-) HPTLC antioxidant PPARγ |
| title | High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts |
| title_full | High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts |
| title_fullStr | High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts |
| title_full_unstemmed | High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts |
| title_short | High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in <i>Periploca aphylla</i> Extracts |
| title_sort | high performance thin layer chromatography for rutin chlorogenic acid caffeic acid ursolic acid and stigmasterol analysis in i periploca aphylla i extracts |
| topic | <i>Periploca aphylla</i> Reverse phase (RP-) Normal Phase (NP-) HPTLC antioxidant PPARγ |
| url | https://www.mdpi.com/2297-8739/8/4/44 |
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